جيل من التمييزية مونوكلونل البشرية من خلايا نادرة محددة مستضد ب المتداولة في الدم

1:17

Isolation of Human Peripheral Blood Mononuclear Cells

2:00

Tetramer-Associated Magnetic Enrichment of Antigen Specific B-Cells

3:58

Staining of Antigen Specific Human B-Cells and Cell Sorting

4:56

Single Cell RT-PCR

6:00

Screening and Verification of Heavy and Light Chain Expressing Vectors

7:45

Production of Monoclonal Antibodies

10:33

Results: Detection of Relevant Antigen Specific B-Cells and Generation of Highly Discriminative Monoclonal Antibody from Donor Peripheral Blood Mononuclear Cells

12:41

Conclusion