في المختبر قياس إنزيم لاختبار الاستجابة الدوائية والوصيفة في فابري ومرض بومبي

0:35

Site-directed Mutagenesis

1:45

Transformation and Screening for the Desired Clone

2:35

pcDNA3.1/GLA and pcDNA3.1/GAA Plasmid Transfection and Treatment of HEK293H

4:00

Cell Harvest

4:43

Homogenization by Freezing and Thawing and BCA Assay

6:17

Enzyme Activity Measurement with Artificial 4-Methylumbeliferyl Substrate

8:19

Results: Enzyme Activity Measurement Mutagenized 3 α-galactosidase and 3 Acid α-glucosidase

9:35

Conclusion