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December 13, 2017
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Summary
Automatically generated
لوسيفراس المشفرة وراثيا مراسل غير الغازية شعبية للتعبير الجيني. استخدام لوسيفراس طولية الآلي التصوير مسجل الغاز ودرجة الحرارة الأمثل (التمساح) يتيح تسجيل طولية من الخلايا طرحه تحت طائفة واسعة من الظروف. هنا نعرض كيف يمكن استخدام التمساح في سياق البحوث إيقاعات سيركاديان.
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Cite this Article
Crosby, P., Hoyle, N. P., O'Neill, J. S. Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder (ALLIGATOR). J. Vis. Exp. (130), e56623, doi:10.3791/56623 (2017).
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