تنقية وإعادة تشكيل TRPV1 لتحليل سبيكتروسكوبيك

0:54

TRPV1 Mutagenesis

2:19

Generating the Recombinant Bacmid and Baculovirus for Protein Expression

5:14

eTRPV1 and Single-cysteine Mutant Purification

7:18

eTRPV1 Single-cysteine Mutant Site-directed Spin Labeling

8:12

eTRPV1 Spin-labeled Single Cysteine Mutant Reconstitution

9:30

Results: Characterization of the Minimal Cysteine-less TRPV1 Construct and Single-cysteine Mutants used for Spectroscopic Analysis

11:08

Conclusion