إعداد الأقسام الفرعية بلاستيدات الخضراء من نبات لتحليل البروتين التعريب Immunoblotting أو البروتيوميات

Harvesting of Arabidopsis Leaves

Purification of Crude Chloroplasts Using Differential Centrifugation

Purification of Intact Chloroplasts on Continuous Percoll Gradient

Lysis of Intact Chloroplasts Using a Hypotonic Buffer and Purification of Chloroplast Sub-Compartments on Discontinuous Sucrose Gradients

Washing and Concentration of Thylakoid and Envelope Membrane Systems

Results: Analysis of Protein Localization by Immunoblotting or Proteomics

Conclusion