الإنتاج ، البلورة ، وتحديد الهيكل للمجال الملزم IKK من نيمو

1:12

Large Scale Expression of Histidine-6 (His₆)-Tagged Nuclear Factor-kappa-B Essential Modulator Crystallography Construct (NEMO-EEAA)

2:33

His₆-Tagged NEMO-EEAA Purification

4:42

His₆ Tag Cleavage and Purification

7:09

Sparse Matrix Screening

8:23

Seed Stock Generation

9:00

Crystal Looping

9:43

X-Ray Data Processing and Structure Solution

10:38

Results: Representative Production, Crystallization, and Structure Determination of the IKK-Binding Domain of NEMO

12:28

Conclusion