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February 12, 2022
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Summary
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تم إنشاء نظام ثقافة عضوية ثلاثية الأبعاد سابقة طويلة الأجل من خلايا الكبد الماوس. يمكن تمرير هذه organoids والتلاعب بها وراثيا عن طريق عدوى الفيروس العدسي من بناء shRNA / خارج الرحم ، انتقال الحمض النووي الريبي والهندسة CRISPR-Cas9.
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Cite this Article
Lian, J., Meng, X., Zhang, X., Hu, H. Establishment and Genetic Manipulation of Murine Hepatocyte Organoids. J. Vis. Exp. (180), e62438, doi:10.3791/62438 (2022).
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