isolation-blood-vessel-derived-multipotent-precursors-from-human

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization&#160;of MSCs have been obscured by their retrospective ...

identifying-amino-acid-overproducers-using-rare-codon-rich-markers

Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers

To satisfy the ever-growing market for amino acids, high-performance production strains are needed. The amino acid overproducers are conventionally ...

multicenter-retrospective-study-blood-flow-restriction-training-after

Multicenter Retrospective Study of Blood Flow Restriction Training After Anterior Cruciate Ligament Reconstruction

Quadriceps weakness and delayed functional recovery are common following anterior cruciate ligament reconstruction (ACLR). Conventional rehabilitation ...

Oregon Health & Science University

beijing institute of technology

Xuzhou Rehabilitation Hospital/The Affiliated Xuzhou Rehabilitation Hospital of Xuzhou Medical University

The translation of rare codons relies on their corresponding rare tRNAs, which could not be fully charged under amino acid starvation. Theoretically, disrupted or retarded translation caused by the lack of charged rare tRNAs can be partially restored by feeding or intracellular synthesis of the corresponding amino acids. Inspired by this assumption, we develop a screening or selection system for obtaining overproducers of a target amino acid by replacing its common codons with the corresponding synonymous rare alternative in the coding sequence of selected reporter proteins or antibiotic-resistant markers. Results show that integration of rare codons can inhibit gene translations in a frequency-dependent manner. As a proof-of-concept, Escherichia coli strains overproducing L-leucine, L-arginine or L-serine are successfully selected from random mutation libraries. The system is also applied to Corynebacterium glutamicum to screen out L-arginine overproducers. This strategy sheds new light on obtaining and understanding amino acid overproduction strains.

ArticleTotal : 3	Year
Publication title Cited by 21	2014
Publication title Cited by 5	2019
Publication title Cited by 1	2025

Bo Zheng

Biography

JoVE Journal Publications

Other Publications