Rainer Fischer

Institute for Molecular Biotechnology, RWTH Aachen University

Affiliated withRWTH Aachen University Fraunhofer Gesellschaft Fraunhofer Institute for Molecular Biology and Applied Ecology

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JoVE Journal Publications

ArticleTotal : 3	Year
Publication title Cited by 32	2014
Publication title Cited by 9	2014
Publication title Cited by 27	2016

Other Publications

Article	Year
A carcinoembryonic antigen-specific diabody produced in tobacco. FASEB journal : official publication of the Federation of American Societies for Experimental Biology\| PubMed ID: 11790722	2002
Uptake of a fluorescent marker in plant cells is sensitive to brefeldin A and wortmannin. The Plant cell\| PubMed ID: 11826300	2002
Intracellular concentration measurements in adherent cells: a comparison of import efficiencies of cell-permeable peptides. Biological chemistry\| PubMed ID: 11934267	2002
Plantibodies: applications, advantages and bottlenecks. Current opinion in biotechnology\| PubMed ID: 11950570	2002
Construction and characterization of a single-chain antibody fragment derived from thymus of a patient with myasthenia gravis. Autoimmunity\| PubMed ID: 12071435	2002
Targeting tryptophan decarboxylase to selected subcellular compartments of tobacco plants affects enzyme stability and in vivo function and leads to a lesion-mimic phenotype. Plant physiology\| PubMed ID: 12114570	2002
A quantitative validation of fluorophore-labelled cell-permeable peptide conjugates: fluorophore and cargo dependence of import. Biochimica et biophysica acta\| PubMed ID: 12175919	2002
Production of tumor-specific antibodies in tobacco. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 12412492	2003
Simultaneous occurrence of Hodgkin's lymphoma and eosinophilic granuloma: a potential pitfall in positron emission tomography imaging. Clinical lymphoma\| PubMed ID: 12435286	2002
Production of antibodies in plants and their use for global health. Vaccine\| PubMed ID: 12531368	2003
'Molecular farming' of antibodies in plants. Die Naturwissenschaften\| PubMed ID: 12712248	2003
The glutathione synthetase of Schizosaccharomyces pombe is synthesized as a homodimer but retains full activity when present as a heterotetramer. The Journal of biological chemistry\| PubMed ID: 12734194	2003
Extending the applicability of carboxyfluorescein in solid-phase synthesis. Bioconjugate chemistry\| PubMed ID: 12757391	2003
An unusual internal ribosomal entry site of inverted symmetry directs expression of a potato leafroll polerovirus replication-associated protein. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 12835413	2003
A young patient with end-stage renal disease, dyspnoea, weakness, peripheral neuropathy and an unsuspected underlying disease. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association\| PubMed ID: 12897114	2003
The recombinant anti-EGF receptor immunotoxin 425(scFv)-ETA' suppresses growth of a highly metastatic pancreatic carcinoma cell line. International journal of oncology\| PubMed ID: 12964002	2003
Production of therapeutic antibodies in plants. Expert opinion on biological therapy\| PubMed ID: 14519078	2003
Molecular farming in plants: host systems and expression technology. Trends in biotechnology\| PubMed ID: 14624867	2003
Recombinant CD64-specific single chain immunotoxin exhibits specific cytotoxicity against acute myeloid leukemia cells. Cancer research\| PubMed ID: 14679004	2003
A stepwise dissection of the intracellular fate of cationic cell-penetrating peptides. The Journal of biological chemistry\| PubMed ID: 14707144	2004
Structure property analysis of pentamethine indocyanine dyes: identification of a new dye for life science applications. Bioconjugate chemistry\| PubMed ID: 14733585	2004
Over-expression and production of plant allergens by molecular farming strategies. Methods (San Diego, Calif.)\| PubMed ID: 14962757	2004
Antibody production in transgenic plants. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 14970504	2004
Plant-based production of biopharmaceuticals. Current opinion in plant biology\| PubMed ID: 15003215	2004
Viroid-induced RNA silencing of GFP-viroid fusion transgenes does not induce extensive spreading of methylation or transitive silencing. The Plant journal : for cell and molecular biology\| PubMed ID: 15053764	2004
Preliminary X-ray analysis of a human V(H) fragment at 1.8 A resolution. Acta crystallographica. Section D, Biological crystallography\| PubMed ID: 15103154	2004
Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen. Nature biotechnology\| PubMed ID: 15146196	2004
Measurement of antibody-membrane interactions by surface plasmon resonance. International journal of molecular medicine\| PubMed ID: 15375615	2004
Chemolabile cellular microarrays for screening small molecules and peptides. Molecular diversity\| PubMed ID: 15384424	2004
Unexpected deposition patterns of recombinant proteins in post-endoplasmic reticulum compartments of wheat endosperm. Plant physiology\| PubMed ID: 15489278	2004
Plant cell cultures for the production of recombinant proteins. Nature biotechnology\| PubMed ID: 15529167	2004
Recombinant anti-EGFR immunotoxin 425(scFv)-ETA' demonstrates anti-tumor activity against disseminated human pancreatic cancer in nude mice. International journal of molecular medicine\| PubMed ID: 15647848	2005
Generation of human antibody fragments against Streptococcus mutans using a phage display chain shuffling approach. BMC biotechnology\| PubMed ID: 15667665	2005
Production of Desmodus rotundus salivary plasminogen activator alpha1 (DSPAalpha1) in tobacco is hampered by proteolysis. Biotechnology and bioengineering\| PubMed ID: 15685597	2005
Reversible cross-linking of hyperbranched polymers: a strategy for the combinatorial decoration of multivalent scaffolds. Angewandte Chemie (International ed. in English)\| PubMed ID: 15688351	2005
Opportunities for recombinant antigen and antibody expression in transgenic plants--technology assessment. Vaccine\| PubMed ID: 15734038	2005
Transgenic plants in the biopharmaceutical market. Expert opinion on emerging drugs\| PubMed ID: 15757412	2005
Sowing the seeds of success: pharmaceutical proteins from plants. Current opinion in biotechnology\| PubMed ID: 15831382	2005
Peptide microarrays for the detection of molecular interactions in cellular signal transduction. Proteomics\| PubMed ID: 15832366	2005
Autophagy promotes MHC class II presentation of peptides from intracellular source proteins. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 15894616	2005
Molecular farming for new drugs and vaccines. Current perspectives on the production of pharmaceuticals in transgenic plants. EMBO reports\| PubMed ID: 15995674	2005
Cationic cell-penetrating peptides interfere with TNF signalling by induction of TNF receptor internalization. Journal of cell science\| PubMed ID: 16079278	2005
A postcolumn alkalinization procedure enhances the sensitivity of fluorescence detection of fluorescein-labeled substances in RP-HPLC. Analytical biochemistry\| PubMed ID: 16098948	2005
Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells. Transgenic research\| PubMed ID: 16145833	2005
Antigen-specific targeting and elimination of EBV-transformed B cells by allergen toxins. The Journal of allergy and clinical immunology\| PubMed ID: 16210069	2005
Break on through to the other side-biophysics and cell biology shed light on cell-penetrating peptides. Chembiochem : a European journal of chemical biology\| PubMed ID: 16254940	2005
One-step analysis of protein complexes in microliters of cell lysate. Nature methods\| PubMed ID: 16278653	2005
Recombinant soluble human Fcgamma receptor I with picomolar affinity for immunoglobulin G. Biochemical and biophysical research communications\| PubMed ID: 16289041	2005
Plant-derived pharmaceuticals--the road forward. Trends in plant science\| PubMed ID: 16290220	2005
Endosperm-specific co-expression of recombinant soybean ferritin and Aspergillus phytase in maize results in significant increases in the levels of bioavailable iron. Plant molecular biology\| PubMed ID: 16307363	2005
Benefit assessment of preventive medical check-ups in patients suffering from chronic granulomatous disease (CGD). Journal of evaluation in clinical practice\| PubMed ID: 16364103	2005
Images in cardiovascular medicine. Cardiomyopathy resulting from primary hyperoxaluria type II. Circulation\| PubMed ID: 16432059	2006
Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development. Frontiers in plant science\| PubMed ID: 26779197	2015
A CD14 domain with lipopolysaccharide-binding and -neutralizing activity. Chembiochem : a European journal of chemical biology\| PubMed ID: 16444757	2006
Recombinant bovine uteroglobin at 1.6 A resolution: a preliminary X-ray crystallographic analysis. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 16511079	2005
Towards industrial usefulness - cryo-cell-banking of transgenic BY-2 cell cultures. Journal of biotechnology\| PubMed ID: 16530286	2006
Restrictive cardiomyopathy in a patient with primary hyperoxaluria type II. Clinical research in cardiology : official journal of the German Cardiac Society\| PubMed ID: 16598594	2006
The intracellular fate of a recombinant protein is tissue dependent. Plant physiology\| PubMed ID: 16632592	2006
Constitutive knox1 gene expression in dandelion (Taraxacum officinale, Web.) changes leaf morphology from simple to compound. Planta\| PubMed ID: 16683160	2006
Antibody-based metabolic engineering in plants. Journal of biotechnology\| PubMed ID: 16698105	2006
The potential of plant virus vectors for vaccine production. Drugs in R&D\| PubMed ID: 16784246	2006
A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway. Chembiochem : a European journal of chemical biology\| PubMed ID: 16871600	2006
Recombinant anti-hCG antibodies retained in the endoplasmic reticulum of transformed plants lack core-xylose and core-alpha(1,3)-fucose residues. Plant biotechnology journal\| PubMed ID: 17134389	2004
A recombinant multimeric immunoglobulin expressed in rice shows assembly-dependent subcellular localization in endosperm cells. Plant biotechnology journal\| PubMed ID: 17168904	2005
Immunomodulation of polyamine biosynthesis in tobacco plants has a significant impact on polyamine levels and generates a dwarf phenotype. Plant biotechnology journal\| PubMed ID: 17173623	2005
A fluorescence-based synthetic LPS sensor. Journal of the American Chemical Society\| PubMed ID: 17227018	2007
Multivalence and spot heterogeneity in microarray-based measurement of binding constants. Analytical and bioanalytical chemistry\| PubMed ID: 17260137	2007
Endocytosis targets exogenous material selectively to cathepsin S in live human dendritic cells, while cell-penetrating peptides mediate nonselective transport to cysteine cathepsins. Journal of leukocyte biology\| PubMed ID: 17261546	2007
Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension cultures. FASEB journal : official publication of the Federation of American Societies for Experimental Biology\| PubMed ID: 17327362	2007
Thoracic actinomycosis imitating a malignant chest wall tumor. Diagnosis: pulmonary actinomycosis. Journal of pediatric hematology/oncology\| PubMed ID: 17483717	2007
A comprehensive model for the cellular uptake of cationic cell-penetrating peptides. Traffic (Copenhagen, Denmark)\| PubMed ID: 17587406	2007
Design of protein membrane interaction inhibitors by virtual ligand screening, proof of concept with the C2 domain of factor V. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 17646652	2007
Spatial and temporal regulation of the forisome gene for1 in the phloem during plant development. Plant molecular biology\| PubMed ID: 17694275	2007
Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans. Plant biotechnology journal\| PubMed ID: 17979949	2008
Cup-like acute myeloid leukemia: new disease or artificial phenomenon? Haematologica\| PubMed ID: 18223289	2008
Eukaryotic expression and secretion of EGFP-labeled annexin A5. Protein expression and purification\| PubMed ID: 18261921	2008
Depletion of autoreactive B-lymphocytes by a recombinant myelin oligodendrocyte glycoprotein-based immunotoxin. Journal of neuroimmunology\| PubMed ID: 18280586	2008
Biochemical and functional characterization of anti-HIV antibody-ELP fusion proteins from transgenic plants. Plant biotechnology journal\| PubMed ID: 18312505	2008
Adenoviral p53 gene transfer and gemcitabine in three patients with liver metastases due to advanced pancreatic carcinoma. HPB : the official journal of the International Hepato Pancreato Biliary Association\| PubMed ID: 18333108	2007
Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 18337510	2008
Small cleavable adapters enhance the specific cytotoxicity of a humanized immunotoxin directed against CD64-positive cells. Journal of immunotherapy (Hagerstown, Md. : 1997)\| PubMed ID: 18391759	2008
Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus 3. Archives of virology\| PubMed ID: 18478181	2008
Plantibody-mediated inhibition of the Potato leafroll virus P1 protein reduces virus accumulation. Virus research\| PubMed ID: 18573562	2008
Molecular farming of human tissue transglutaminase in tobacco plants. Amino acids\| PubMed ID: 18594943	2009
Evolution of a regulatory framework for pharmaceuticals derived from genetically modified plants. Trends in biotechnology\| PubMed ID: 18676047	2008
Engineering Fusarium head blight resistance in wheat by expression of a fusion protein containing a Fusarium-specific antibody and an antifungal peptide. Molecular plant-microbe interactions : MPMI\| PubMed ID: 18700828	2008
HPMA as a scaffold for the modular assembly of functional peptide polymers by native chemical ligation. Bioconjugate chemistry\| PubMed ID: 18778092	2008
Phage-display derived single-chain fragment variable (scFv) antibodies recognizing conformational epitopes of Escherichia coli heat-labile enterotoxin B-subunit. Journal of immunological methods\| PubMed ID: 18786540	2008
Granzyme B-H22(scFv), a human immunotoxin targeting CD64 in acute myeloid leukemia of monocytic subtypes. Molecular cancer therapeutics\| PubMed ID: 18790773	2008
An assay for the detection of grapevine leafroll-associated virus 3 using a single-chain fragment variable antibody. Archives of virology\| PubMed ID: 19082687	2009
Grapevine fanleaf virus (GFLV)-specific antibodies confer GFLV and Arabis mosaic virus (ArMV) resistance in Nicotiana benthamiana. Molecular plant pathology\| PubMed ID: 19161351	2009
Recombinant-antibody-mediated resistance against Tomato yellow leaf curl virus in Nicotiana benthamiana. Archives of virology\| PubMed ID: 19234665	2009
Epstein-Barr virus BDLF2-BMRF2 complex affects cellular morphology. The Journal of general virology\| PubMed ID: 19264620	2009
Methyl jasmonate induced accumulation of kalopanaxsaponin I in Nigella sativa. Phytochemistry\| PubMed ID: 19282005	2009
Recombinant bispecific single chain antibody fragments induce Fc gamma-receptor-mediated elimination of CD30+ lymphoma cells. Cancer letters\| PubMed ID: 19345477	2009
Polyphenoloxidase silencing affects latex coagulation in Taraxacum species. Plant physiology\| PubMed ID: 19605551	2009
The structure of the dizinc subclass B2 metallo-beta-lactamase CphA reveals that the second inhibitory zinc ion binds in the histidine site. Antimicrobial agents and chemotherapy\| PubMed ID: 19651913	2009
Influence of elastin-like peptide fusions on the quantity and quality of a tobacco-derived human immunodeficiency virus-neutralizing antibody. Plant biotechnology journal\| PubMed ID: 19843249	2009
Genetic determinants of Pseudomonas aeruginosa biofilm establishment. Microbiology (Reading, England)\| PubMed ID: 19850623	2010
A cell-penetrating peptide derived from human lactoferrin with conformation-dependent uptake efficiency. The Journal of biological chemistry\| PubMed ID: 19858187	2009
A doubly labeled penetratin analogue as a ratiometric sensor for intracellular proteolytic stability. Bioconjugate chemistry\| PubMed ID: 19957912	2010
Potato virus X as a novel platform for potential biomedical applications. Nano letters\| PubMed ID: 20017489	2010
Characterization and diagnostic potential of foreign epitope-presenting Ty1 virus-like particles expressed in Escherichia coli and Pichia pastoris. Journal of molecular microbiology and biotechnology\| PubMed ID: 20068357	2010
Generation and characterization of a scFv against recombinant coat protein of the geminivirus tomato leaf curl New Delhi virus. Archives of virology\| PubMed ID: 20107847	2010
Immunogenic properties of chimeric potato virus X particles displaying the hepatitis C virus hypervariable region I peptide R9. Journal of virological methods\| PubMed ID: 20138085	2010
Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz. BMC biochemistry\| PubMed ID: 20170509	2010
The changing fate of a secretory glycoprotein in developing maize endosperm. Plant physiology\| PubMed ID: 20388665	2010
Rapid optical imaging of EGF receptor expression with a single-chain antibody SNAP-tag fusion protein. European journal of nuclear medicine and molecular imaging\| PubMed ID: 20449589	2010
Emphysematous gastritis caused by Sarcina ventriculi. Gastrointestinal endoscopy\| PubMed ID: 20538273	2010
Biochemical properties of the matrix metalloproteinase NtMMP1 from Nicotiana tabacum cv. BY-2 suspension cells. Planta\| PubMed ID: 20635096	2010
A monoclonal antibody that specifically binds chitosan in vitro and in situ on fungal cell walls. Journal of microbiology and biotechnology\| PubMed ID: 20798578	2010
Rapid transient production in plants by replicating and non-replicating vectors yields high quality functional anti-HIV antibody. PloS one\| PubMed ID: 21103044	2010
GMP issues for recombinant plant-derived pharmaceutical proteins. Biotechnology advances\| PubMed ID: 21856403	2012
Harmonine, a defence compound from the harlequin ladybird, inhibits mycobacterial growth and demonstrates multi-stage antimalarial activity. Biology letters\| PubMed ID: 21937493	2012
How recombinant swollenin from Kluyveromyces lactis affects cellulosic substrates and accelerates their hydrolysis. Biotechnology for biofuels\| PubMed ID: 21943248	2011
In vivo imaging of immunotoxin treatment using Katushka-transfected A-431 cells in a murine xenograft tumour model. Cancer immunology, immunotherapy : CII\| PubMed ID: 22350071	2012
Generation and comparative characterization of glycosylated and aglycosylated human IgG1 antibodies. Molecular biotechnology\| PubMed ID: 22427250	2013
In vivo expression and binding activity of scFv-RWAV, which recognizes the coat protein of tomato leaf curl New Delhi virus (family Geminiviridae). Archives of virology\| PubMed ID: 22491815	2012
Development of viral nanoparticles for efficient intracellular delivery. Nanoscale\| PubMed ID: 22508503	2012
Proteomic analysis of latex from the rubber-producing plant Taraxacum brevicorniculatum. Proteomics\| PubMed ID: 22539439	2012
Monoclonal tobacco cell lines with enhanced recombinant protein yields can be generated from heterogeneous cell suspension cultures by flow sorting. Plant biotechnology journal\| PubMed ID: 22758383	2012
Proteins from the FLOWERING LOCUS T-like subclade of the PEBP family act antagonistically to regulate floral initiation in tobacco. The Plant journal : for cell and molecular biology\| PubMed ID: 22889438	2012
Processing heterogeneous biomass: overcoming the hurdles in model building. Bioengineered\| PubMed ID: 22895059	2013
Hyperthermophilic endoglucanase for in planta lignocellulose conversion. Biotechnology for biofuels\| PubMed ID: 22928996	2012
Histone acetylation mediates epigenetic regulation of transcriptional reprogramming in insects during metamorphosis, wounding and infection. Frontiers in zoology\| PubMed ID: 23035888	2012
A monoclonal antibody for the detection of SNAP/CLIP-tagged proteins. Immunology letters\| PubMed ID: 23085606	2013
With or without sugar? (A)glycosylation of therapeutic antibodies. Molecular biotechnology\| PubMed ID: 23097175	2013
Flow cytometry-based ultra-high-throughput screening assay for cellulase activity. Analytical biochemistry\| PubMed ID: 23146590	2013
Malaria parasites co-opt human factor H to prevent complement-mediated lysis in the mosquito midgut. Cell host & microbe\| PubMed ID: 23332154	2013
Brain infection and activation of neuronal repair mechanisms by the human pathogen Listeria monocytogenes in the lepidopteran model host Galleria mellonella. Virulence\| PubMed ID: 23348912	2013
Commercial aspects of pharmaceutical protein production in plants. Current pharmaceutical design\| PubMed ID: 23394566	2013
Optimizing the yield of recombinant pharmaceutical proteins in plants. Current pharmaceutical design\| PubMed ID: 23394567	2013
Molecular farming of pharmaceutical proteins using plant suspension cell and tissue cultures. Current pharmaceutical design\| PubMed ID: 23394569	2013
Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems. PloS one\| PubMed ID: 23533588	2013
Yeast surface display for the expression, purification and characterization of wild-type and B11 mutant glucose oxidases. Protein expression and purification\| PubMed ID: 23562736	2013
Changes in the transcriptome of the malaria parasite Plasmodium falciparum during the initial phase of transmission from the human to the mosquito. BMC genomics\| PubMed ID: 23586929	2013
Ethanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development. Biotechnology for biofuels\| PubMed ID: 23587418	2013
Galleria mellonella as a model host to study gut microbe homeostasis and brain infection by the human pathogen listeria monocytogenes. Advances in biochemical engineering/biotechnology\| PubMed ID: 23708825	2013
Optimization of BY-2 cell suspension culture medium for the production of a human antibody using a combination of fractional factorial designs and the response surface method. Plant biotechnology journal\| PubMed ID: 23721307	2013
Cellulases from insects. Advances in biochemical engineering/biotechnology\| PubMed ID: 23728162	2013
Characterization and dynamic behavior of wild yeast during spontaneous wine fermentation in steel tanks and amphorae. BioMed research international\| PubMed ID: 23738327	2013
Cellulases for biomass degradation: comparing recombinant cellulase expression platforms. Trends in biotechnology\| PubMed ID: 23910542	2013
An efficient process for the saccharification of wood chips by combined ionic liquid pretreatment and enzymatic hydrolysis. Bioresource technology\| PubMed ID: 23933021	2013
Granzyme M as a novel effector molecule for human cytolytic fusion proteins: CD64-specific cytotoxicity of Gm-H22(scFv) against leukemic cells. Cancer letters\| PubMed ID: 23973499	2013
Transcriptome alterations in zebrafish embryos after exposure to environmental estrogens and anti-androgens can reveal endocrine disruption. Reproductive toxicology (Elmsford, N.Y.)\| PubMed ID: 24051129	2013
Cloning, Heterologous Expression, Purification and Characterization of M12 Mutant of Aspergillus niger Glucose Oxidase in Yeast Pichia pastoris KM71H. Molecular biotechnology\| PubMed ID: 24122283	2013
A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach. Bioprocess and biosystems engineering\| PubMed ID: 24136042	2013
Human microtubule-associated protein tau mediates targeted killing of CD30(+) lymphoma cells in vitro and inhibits tumour growth in vivo. British journal of haematology\| PubMed ID: 24164493	2014
Flocculation increases the efficacy of depth filtration during the downstream processing of recombinant pharmaceutical proteins produced in tobacco. Plant biotechnology journal\| PubMed ID: 24165151	2014
Genetic engineering and chemical conjugation of potato virus X. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 24243237	2014
Plant-based production of recombinant Plasmodium surface protein pf38 and evaluation of its potential as a vaccine candidate. PloS one\| PubMed ID: 24278216	2013
Isolation and characterization of isochorismate synthase and cinnamate 4-hydroxylase during salinity stress, wounding, and salicylic acid treatment in Carthamus tinctorius. Plant signaling & behavior\| PubMed ID: 24309561	2013
Scale-down models to optimize a filter train for the downstream purification of recombinant pharmaceutical proteins produced in tobacco leaves. Biotechnology journal\| PubMed ID: 24323869	2013
Protective Oral Vaccination against Infectious bursal disease virus Using the Major Viral Antigenic Protein VP2 Produced in Pichia pastoris. PloS one\| PubMed ID: 24376665	2013
Generic chromatography-based purification strategies accelerate the development of downstream processes for biopharmaceutical proteins produced in plants. Biotechnology journal\| PubMed ID: 24478119	2014
The p38-mediated rapid down-regulation of cell surface gp130 expression impairs interleukin-6 signaling in the synovial fluid of juvenile idiopathic arthritis patients. Arthritis & rheumatology (Hoboken, N.J.)\| PubMed ID: 24504821	2014
Metabolic engineering for p-coumaryl alcohol production in Escherichia coli by introducing an artificial phenylpropanoid pathway. Biotechnology and applied biochemistry\| PubMed ID: 24575890	2014
Plant molecular pharming for the treatment of chronic and infectious diseases. Annual review of plant biology\| PubMed ID: 24579993	2014
Perforin-like protein PPLP2 permeabilizes the red blood cell membrane during egress of Plasmodium falciparum gametocytes. Cellular microbiology\| PubMed ID: 24602217	2014
Ultra-high-throughput screening method for the directed evolution of glucose oxidase. Chemistry & biology\| PubMed ID: 24613019	2014
Downstream processing of biopharmaceutical proteins produced in plants: The pros and cons of flocculants. Bioengineered\| PubMed ID: 24637706	2014
pH-dependent nano-capturing of tartaric acid using dendrimers. Soft matter\| PubMed ID: 24651997	2014
Inhibition of protease activity by antisense RNA improves recombinant protein production in Nicotiana tabacum cv Bright Yellow 2 (BY-2) suspension cells. Biotechnology journal\| PubMed ID: 24828029	2014
Differential inductions of phenylalanine ammonia-lyase and chalcone synthase during wounding, salicylic acid treatment, and salinity stress in safflower, Carthamus tinctorious. Bioscience reports\| PubMed ID: 24865400	2014
Alternative intronic polyadenylation generates the interleukin-6 trans-signaling inhibitor sgp130-E10. The Journal of biological chemistry\| PubMed ID: 24973212	2014
EpCAM-selective elimination of carcinoma cells by a novel MAP-based cytolytic fusion protein. Molecular cancer therapeutics\| PubMed ID: 24980949	2014
Species-specific considerations in using the fish embryo test as an alternative to identify endocrine disruption. Aquatic toxicology (Amsterdam, Netherlands)\| PubMed ID: 24992288	2014
A CSPG4-specific immunotoxin kills rhabdomyosarcoma cells and binds to primary tumor tissues. Cancer letters\| PubMed ID: 25016058	2014
Assessment of cultivation factors that affect biomass and geraniol production in transgenic tobacco cell suspension cultures. PloS one\| PubMed ID: 25117009	2014
Scaled-up manufacturing of recombinant antibodies produced by plant cells in a 200-L orbitally-shaken disposable bioreactor. Biotechnology and bioengineering\| PubMed ID: 25117428	2015
Assessment of the neutrophilic antibody-dependent respiratory burst (ADRB) response to Plasmodium falciparum. Journal of leukocyte biology\| PubMed ID: 25118179	2014
Potato virus X-based expression vectors are stabilized for long-term production of proteins and larger inserts. Biotechnology journal\| PubMed ID: 25171768	2014
Inhibition of the SR protein-phosphorylating CLK kinases of Plasmodium falciparum impairs blood stage replication and malaria transmission. PloS one\| PubMed ID: 25188378	2014
Malaria vaccine candidate antigen targeting the pre-erythrocytic stage of Plasmodium falciparum produced at high level in plants. Biotechnology journal\| PubMed ID: 25200253	2014
Detailed functional characterization of glycosylated and nonglycosylated variants of malaria vaccine candidate PfAMA1 produced in Nicotiana benthamiana and analysis of growth inhibitory resp... Plant biotechnology journal\| PubMed ID: 25236489	2015
Synthesis and characterization of an insoluble polymer based on polyamidoamine: applications for the decontamination of metals in aqueous systems. Journal of environmental management\| PubMed ID: 25304521	2015
Antimalarial activity of granzyme B and its targeted delivery by a granzyme B-single-chain Fv fusion protein. Antimicrobial agents and chemotherapy\| PubMed ID: 25313223	2015
Optimization of a multi-stage, multi-subunit malaria vaccine candidate for the production in Pichia pastoris by the identification and removal of protease cleavage sites. Biotechnology and bioengineering\| PubMed ID: 25335451	2015
Challenges and advances in the heterologous expression of cellulolytic enzymes: a review. Biotechnology for biofuels\| PubMed ID: 25356086	2014
A high-throughput cellulase screening system based on droplet microfluidics. Biomicrofluidics\| PubMed ID: 25379082	2014
In vitro effects and ex vivo binding of an EGFR-specific immunotoxin on rhabdomyosarcoma cells. Journal of cancer research and clinical oncology\| PubMed ID: 25433506	2015
Recombinant H22(scFv) blocks CD64 and prevents the capture of anti-TNF monoclonal antibody. A potential strategy to enhance anti-TNF therapy. mAbs\| PubMed ID: 25517313	2014
The CRISPR/Cas9 system for plant genome editing and beyond. Biotechnology advances\| PubMed ID: 25536441	2015
Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens. Immunologic research\| PubMed ID: 25537533	2015
Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D. Frontiers in immunology\| PubMed ID: 25538707	2014
Synthetic polymers are more effective than natural flocculants for the clarification of tobacco leaf extracts. Journal of biotechnology\| PubMed ID: 25545028	2015
Cloning murine antibody V-genes with non-degenerate primers and conversion to a recombinant antibody format. Monoclonal antibodies in immunodiagnosis and immunotherapy\| PubMed ID: 25545205	2014
High-value products from plants: the challenges of process optimization. Current opinion in biotechnology\| PubMed ID: 25562816	2015
The increasing value of plant-made proteins. Current opinion in biotechnology\| PubMed ID: 25578557	2015
Cellulose-based filter aids increase the capacity of depth filters during the downstream processing of plant-derived biopharmaceutical proteins. Biotechnology journal\| PubMed ID: 25611947	2015
Heat-precipitation allows the efficient purification of a functional plant-derived malaria transmission-blocking vaccine candidate fusion protein. Biotechnology and bioengineering\| PubMed ID: 25615702	2015
Next-generation sequencing is a robust strategy for the high-throughput detection of zygosity in transgenic maize. Transgenic research\| PubMed ID: 25648956	2015
Fast track antibody V-gene rescue, recombinant expression in plants and characterization of a PfMSP4-specific antibody. Malaria journal\| PubMed ID: 25651860	2015
Angiogenin mutants as novel effector molecules for the generation of fusion proteins with increased cytotoxic potential. Journal of immunotherapy (Hagerstown, Md. : 1997)\| PubMed ID: 25710248	2015
Simple and portable magnetic immunoassay for rapid detection and sensitive quantification of plant viruses. Applied and environmental microbiology\| PubMed ID: 25710366	2015
Pharmacokinetics and immunogenicity of broadly neutralizing HIV monoclonal antibodies in macaques. PloS one\| PubMed ID: 25807114	2015
The three-dimensional structure of VIM-31--a metallo-β-lactamase from Enterobacter cloacae in its native and oxidized form. The FEBS journal\| PubMed ID: 25825035	2015
Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei. BMC plant biology\| PubMed ID: 25849300	2015
Targeted killing of rhabdomyosarcoma cells by a MAP-based human cytolytic fusion protein. Cancer letters\| PubMed ID: 25888452	2015
Novel EGFR-specific immunotoxins based on panitumumab and cetuximab show in vitro and ex vivo activity against different tumor entities. Journal of cancer research and clinical oncology\| PubMed ID: 25899161	2015
The stage-specific in vitro efficacy of a malaria antigen cocktail provides valuable insights into the development of effective multi-stage vaccines. Biotechnology journal\| PubMed ID: 25913888	2015
Synthesis and evaluation of the antiplasmodial activity of tryptanthrin derivatives. International journal for parasitology. Drugs and drug resistance\| PubMed ID: 25949928	2015
The Fc-alpha receptor is a new target antigen for immunotherapy of myeloid leukemia. International journal of cancer\| PubMed ID: 26041304	2015
Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays). Transgenic research\| PubMed ID: 26071308	2015
Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants. Plant biotechnology journal\| PubMed ID: 26147010	2015
Analysis of a Multi-component Multi-stage Malaria Vaccine Candidate--Tackling the Cocktail Challenge. PloS one\| PubMed ID: 26147206	2015
Perforin-like protein PPLP4 is crucial for mosquito midgut infection by Plasmodium falciparum. Molecular and biochemical parasitology\| PubMed ID: 26166358	2015
Isolation, production and characterization of fully human monoclonal antibodies directed to Plasmodium falciparum MSP10. Malaria journal\| PubMed ID: 26174014	2015
From gene to harvest: insights into upstream process development for the GMP production of a monoclonal antibody in transgenic tobacco plants. Plant biotechnology journal\| PubMed ID: 26214282	2015
Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies. mAbs\| PubMed ID: 26218624	2015
Targeted gene exchange in plant cells mediated by a zinc finger nuclease double cut. Plant biotechnology journal\| PubMed ID: 26426390	2016
Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X. Macromolecular bioscience\| PubMed ID: 26440117	2016
The Plasmodium falciparum blood stages acquire factor H family proteins to evade destruction by human complement. Cellular microbiology\| PubMed ID: 26457721	2016
Novel angiogenin mutants with increased cytotoxicity enhance the depletion of pro-inflammatory macrophages and leukemia cells ex vivo. Cancer immunology, immunotherapy : CII\| PubMed ID: 26472728	2015
A WD40-repeat protein unique to malaria parasites associates with adhesion protein complexes and is crucial for blood stage progeny. Malaria journal\| PubMed ID: 26537493	2015
Antibody-Mediated Pathogen Resistance in Plants. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 26614296	2016
Plant expression and characterization of the transmission-blocking vaccine candidate PfGAP50. BMC biotechnology\| PubMed ID: 26625934	2015
Developments in the production of mucosal antibodies in plants. Biotechnology advances\| PubMed ID: 26626615	2016
Overcoming low yields of plant-made antibodies by a protein engineering approach. Biotechnology journal\| PubMed ID: 26632507	2016
Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins. Biotechnology journal\| PubMed ID: 26632519	2016
Production of Hybrid Chimeric PVX Particles Using a Combination of TMV and PVX-Based Expression Vectors. Frontiers in bioengineering and biotechnology\| PubMed ID: 26636076	2015
Comparison of Thermobifida fusca Cellulases Expressed in Escherichia coli and Nicotiana tabacum Indicates Advantages of the Plant System for the Expression of Bacterial Cellulases. Frontiers in plant science\| PubMed ID: 26648951	2015
Depth Filters Containing Diatomite Achieve More Efficient Particle Retention than Filters Solely Containing Cellulose Fibers. Frontiers in plant science\| PubMed ID: 26734037	2015
Fully human MAP-fusion protein selectively targets and eliminates proliferating CD64(+) M1 macrophages. Immunology and cell biology\| PubMed ID: 26743033	2016
Development of a Competitive Cystatin C-Specific Bioassay Suitable for Repetitive Measurements. PloS one\| PubMed ID: 26799562	2016
Granzyme B-based cytolytic fusion protein targeting EpCAM specifically kills triple negative breast cancer cells in vitro and inhibits tumor growth in a subcutaneous mouse tumor model. Cancer letters\| PubMed ID: 26806809	2016
Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria. Malaria journal\| PubMed ID: 26850066	2016
Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen. BMC biotechnology\| PubMed ID: 26883813	2016
A novel fully-human cytolytic fusion protein based on granzyme B shows in vitro cytotoxicity and ex vivo binding to solid tumors overexpressing the epidermal growth factor receptor. Cancer letters\| PubMed ID: 26912070	2016
Optimized Blanching Reduces the Host Cell Protein Content and Substantially Enhances the Recovery and Stability of Two Plant-Derived Malaria Vaccine Candidates. Frontiers in plant science\| PubMed ID: 26925077	2016
More for less: Improving the biomass yield of a pear cell suspension culture by design of experiments. Scientific reports\| PubMed ID: 26988402	2016
Putting the Spotlight Back on Plant Suspension Cultures. Frontiers in plant science\| PubMed ID: 27014320	2016
A novel approach for targeted elimination of CSPG4-positive triple-negative breast cancer cells using a MAP tau-based fusion protein. International journal of cancer\| PubMed ID: 27037627	2016
A Plant-Based Transient Expression System for the Rapid Production of Malaria Vaccine Candidates. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 27076325	2016
A Monoclonal Antibody That Discriminates Between SNAP-Tagged and CLIP-Tagged Proteins. Monoclonal antibodies in immunodiagnosis and immunotherapy\| PubMed ID: 27187007	2016
Analysis of the dose-dependent stage-specific in vitro efficacy of a multi-stage malaria vaccine candidate cocktail. Malaria journal\| PubMed ID: 27188716	2016
Differences in toxicity of anionic and cationic PAMAM and PPI dendrimers in zebrafish embryos and cancer cell lines. Toxicology and applied pharmacology\| PubMed ID: 27288734	2016
Progress and obstacles in the production and application of recombinant lignin-degrading peroxidases. Bioengineered\| PubMed ID: 27295524	2016
Controlled Release of Nor-β-lapachone by PLGA Microparticles: A Strategy for Improving Cytotoxicity against Prostate Cancer Cells. Molecules (Basel, Switzerland)\| PubMed ID: 27384551	2016
SNAP-Tag Technology: A Useful Tool To Determine Affinity Constants and Other Functional Parameters of Novel Antibody Fragments. Bioconjugate chemistry\| PubMed ID: 27391930	2016
Photoimmunotheranostic agents for triple-negative breast cancer diagnosis and therapy that can be activated on demand. Oncotarget\| PubMed ID: 27448975	2016