human-cartilage-tissue-fabrication-using-three-dimensional-inkjet

Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology

Bioprinting, which is based on thermal inkjet printing, is one of the most attractive enabling technologies in the field of tissue engineering and ...

Stemorgan Inc.

wuhan university

Our previous study has suggested thatthe bladder cancer-associated protein gene (BLCAP) was among the differentially expressed genes in cervical cancer. We confirm here that BLCAP is expressed in all noncancerous cervical tissues (10/10), but it is greatly lost in primary cervical cancer tissue (31/39). In order to further investigate the functional roles of BLCAP, we stably transfected BLCAP cDNA into HeLa cells. The HeLa cells expressing BLCAP show reduced cell growth and clone genicity compared to the vector-transfected cognate cells. BLCAP expression in HeLa cells leads to growth arrest and significantly enhanced apoptosis in vitro and reduced tumor formation in vivo. Thus, BLCAP might be a potential tumor suppressor gene in cervical carcinoma.

Functional analysis of bladder cancer-related protein gene: a putative cervical cancer tumor suppressor gene in cervical carcinoma.

In previous studies, we firstly isolated a new human papillomavirus type 16 E7 variant (HBE7.16) from cervical cancer biopsies of patients from Wufeng County of Hubei Province, China, where the mortality rate of cervical cancer ranks the highest in China. We identified 2 mutations in HBE7.16 compared with the prototype E7 gene (E7.16). The more relevant mutation produces a termination codon, resulting in a truncated E7 protein containing 43 amino acids, making it about half the size of the wild-type. In this study, we investigated the biological function of this HBE7.16 protein. We compared the transforming potential of HBE7.16 to E7.16 in National Institute of Health (NIH) 3T3 cells using cell proliferation and anchorage-independent growth assays. We also examined the expression level of cdc25A, cyclin A, and cyclin E in transformed cells using semi-quantitative reverse transcription-polymerase chain reaction. The results showed that E7.16 and HBE7.16 significantly transform NIH3T3 cells, and HBE7.16 holds greater transforming activity than E7.16. Cells expressing HBE7.16 can more easily transit from G1 into S phase and express higher levels of cdc25A and cyclin A compared with E7.16. No significant difference in the expression level of cyclin E was seen. We propose that up-regulation of cdc25A and cyclin A contributes, at least partially, to E7.16- or HBE7.16-induced transformation of NIH 3T3 cells. In addition, higher transforming ability of HBE7.16 may be responsible for the increased mortality of cervical cancer in Wufeng County.

Human papillomavirus 16 variant E7 gene induces transformation of NIH 3T3 cells via up-regulation of cdc25A and cyclin A.

Muscle-powered, biological, microelectro-mechanical system is promising for actuator and biosensor applications. Functional conjugation between the cells, tissues, and biomolecules to the microdevice is crucial for this application. Bioprinting as an enabling technology possesses the advantages of high throughput, digital control, and highly accurate delivery of various biological factors to the desired locations for numerous applications such as 3D tissue fabrication. We have now evaluated the feasibility of the precise placement of mouse myoblasts onto micro-sized cantilevers. The evenly aligned printed cells fused with each other and formed mature myotubes after only 4 days. In contrast, it took more than 14 days for randomly deposited cells to do so. The printed myotubes were functional and responded to the electrical stimulation synchronously. Furthermore, the integrated Bio-MEMS device responded to the chemical stimulation spontaneously which demonstrated the potential as a functional biosensor. The contractility of the system was recovered quickly after the removal of the chemical stimulation, which indicated the flexibility of this system and the recycling potential.

Article	Year
Functional analysis of bladder cancer-related protein gene: a putative cervical cancer tumor suppressor gene in cervical carcinoma. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine\| PubMed ID: 16675915	2006
Human papillomavirus 16 variant E7 gene induces transformation of NIH 3T3 cells via up-regulation of cdc25A and cyclin A. International journal of gynecological cancer : official journal of the International Gynecological Cancer Society\| PubMed ID: 19509542	2009
Accelerated myotube formation using bioprinting technology for biosensor applications. Biotechnology letters\| PubMed ID: 23160742	2013

Guifang Gao

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