Ashutosh Chilkoti

Department of Biomedical Engineering, Duke University

Research Area

Biography

Ashutosh Chilkoti has not added Biography.
If you are Ashutosh Chilkoti and would like to personalize this page please email our Author Liaison for assistance.

JoVE Journal Publications

ArticleTotal : 1	Year
Publication title Cited by 48	2014

Other Publications

Article	Year
A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface. Analytical chemistry\| PubMed ID: 11838667	2002
Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: examples from the elastin-like polypeptide s... Biomacromolecules\| PubMed ID: 11888323	2002
Targeted drug delivery by thermally responsive polymers. Advanced drug delivery reviews\| PubMed ID: 12204595	2002
Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair. Biomacromolecules\| PubMed ID: 12217035	2002
Design of thermally responsive, recombinant polypeptide carriers for targeted drug delivery. Advanced drug delivery reviews\| PubMed ID: 12384309	2002
Effect of streptavidin affinity mutants on the integrin-independent adhesion of biotinylated endothelial cells. Biomaterials\| PubMed ID: 12437950	2003
Fabrication of a reversible protein array directly from cell lysate using a stimuli-responsive polypeptide. Analytical chemistry\| PubMed ID: 12622356	2003
Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides. Biomacromolecules\| PubMed ID: 12741772	2003
Evaluation of an elastin-like polypeptide-doxorubicin conjugate for cancer therapy. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 12932635	2003
Effect of genetic circular permutation near the active site on the activity and stability of an enzyme inhibitor. Biomolecular engineering\| PubMed ID: 14715318	2004
Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold. Journal of the American Chemical Society\| PubMed ID: 15080668	2004
Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides. Biomacromolecules\| PubMed ID: 15132671	2004
Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches". Journal of the American Chemical Society\| PubMed ID: 15186170	2004
Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size. Analytical chemistry\| PubMed ID: 15362894	2004
Nitroxide conjugate of a thermally responsive elastin-like polypeptide for noninvasive thermometry. Medical physics\| PubMed ID: 15543780	2004
Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion. Protein science : a publication of the Protein Society\| PubMed ID: 15557268	2004
Label free colorimetric biosensing using nanoparticles. Journal of fluorescence\| PubMed ID: 15617380	2004
Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide. Biomaterials\| PubMed ID: 16023192	2006
Self-cleavable stimulus responsive tags for protein purification without chromatography. Journal of the American Chemical Society\| PubMed ID: 16089436	2005
Patterning cells in highly deformable microstructures: effect of plastic deformation of substrate on cellular phenotype and gene expression. Biomaterials\| PubMed ID: 16154191	2006
Enzymatic fabrication of DNA nanostructures: extension of a self-assembled oligonucleotide monolayer on gold arrays. Journal of the American Chemical Society\| PubMed ID: 16218572	2005
Epitope tagging for tracking elastin-like polypeptides. Biomaterials\| PubMed ID: 16278015	2006
Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption. Biomacromolecules\| PubMed ID: 16283770	2005
Structural optimization of a "smart" doxorubicin-polypeptide conjugate for thermally targeted delivery to solid tumors. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 16303202	2006
Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair. Tissue engineering\| PubMed ID: 16411822	2005
Tumor vascular permeability, accumulation, and penetration of macromolecular drug carriers. Journal of the National Cancer Institute\| PubMed ID: 16507830	2006
Protein-resistant polymer coatings on silicon oxide by surface-initiated atom transfer radical polymerization. Langmuir : the ACS journal of surfaces and colloids\| PubMed ID: 16584252	2006
Ultra-high expression of a thermally responsive recombinant fusion protein in E. coli. Biotechnology progress\| PubMed ID: 16739944	2006
Purification of an elastin-like fusion protein by microfiltration. Biotechnology and bioengineering\| PubMed ID: 16767781	2006
Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry. Journal of biomedical optics\| PubMed ID: 16822071	2006
Aqueous two-phase system formation kinetics for elastin-like polypeptides of varying chain length. Biomacromolecules\| PubMed ID: 16827587	2006
Tracking the in vivo fate of recombinant polypeptides by isotopic labeling. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 16904221	2006
Tumor accumulation, degradation and pharmacokinetics of elastin-like polypeptides in nude mice. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 16919353	2006
A thermally responsive biopolymer for intra-articular drug delivery. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 16959360	2006
Stimulus responsive elastin biopolymers: Applications in medicine and biotechnology. Current opinion in chemical biology\| PubMed ID: 17055770	2006
Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling. Analytical biochemistry\| PubMed ID: 17084802	2007
Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides. Langmuir : the ACS journal of surfaces and colloids\| PubMed ID: 17190524	2007
Fabrication of gold nanowires by electric-field-induced scanning probe lithography and in situ chemical development. Small (Weinheim an der Bergstrasse, Germany)\| PubMed ID: 17199247	2007
Analysis of total uncertainty in spectral peak measurements for plasmonic nanoparticle-based biosensors. Applied optics\| PubMed ID: 17356640	2007
Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides. Biomacromolecules\| PubMed ID: 17407348	2007
Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution. Biomacromolecules\| PubMed ID: 17411091	2007
Force-induced prolyl cis-trans isomerization in elastin-like polypeptides. Journal of the American Chemical Society\| PubMed ID: 17469821	2007
The effect of covalently immobilized rhIL-1ra-ELP fusion protein on the inflammatory profile of LPS-stimulated human monocytes. Biomaterials\| PubMed ID: 17482260	2007
Thermal cycling enhances the accumulation of a temperature-sensitive biopolymer in solid tumors. Cancer research\| PubMed ID: 17483356	2007
Plasmonic detection of a model analyte in serum by a gold nanorod sensor. Analytical chemistry\| PubMed ID: 17567106	2007
Toward a systems engineering approach to cancer drug delivery. Journal of the National Cancer Institute\| PubMed ID: 17596569	2007
Multifunctional thermally transitioning oligopeptides prepared by ring-opening metathesis polymerization. Biomacromolecules\| PubMed ID: 17625908	2007
Surface-initiated enzymatic polymerization of DNA. Langmuir : the ACS journal of surfaces and colloids\| PubMed ID: 17929953	2007
Development and characterization of a fusion protein between thermally responsive elastin-like polypeptide and interleukin-1 receptor antagonist: sustained release of a local antiinflammator... Arthritis and rheumatism\| PubMed ID: 17968946	2007
Temperature triggered self-assembly of polypeptides into multivalent spherical micelles. Journal of the American Chemical Society\| PubMed ID: 18085778	2008
In situ cross-linking of elastin-like polypeptide block copolymers for tissue repair. Biomacromolecules\| PubMed ID: 18163573	2008
Label-free plasmonic detection of biomolecular binding by a single gold nanorod. Analytical chemistry\| PubMed ID: 18197636	2008
An injectable and in situ-gelling biopolymer for sustained drug release following perineural administration. Spine\| PubMed ID: 18379401	2008
In situ crosslinking elastin-like polypeptide gels for application to articular cartilage repair in a goat osteochondral defect model. Tissue engineering. Part A\| PubMed ID: 18433311	2008
Synthesis and characterization of a thermally-responsive tumor necrosis factor antagonist. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 18547669	2008
Distance-dependent plasmon resonant coupling between a gold nanoparticle and gold film. Nano letters\| PubMed ID: 18590340	2008
Temperature sensitive peptides: engineering hyperthermia-directed therapeutics. International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group\| PubMed ID: 18608590	2008
Hydration and conformational mechanics of single, end-tethered elastin-like polypeptides. Journal of the American Chemical Society\| PubMed ID: 18646848	2008
Effects of Hofmeister anions on the phase transition temperature of elastin-like polypeptides. The journal of physical chemistry. B\| PubMed ID: 18842018	2008
Allosteric actuation of inverse phase transition of a stimulus-responsive fusion polypeptide by ligand binding. Journal of the American Chemical Society\| PubMed ID: 19055326	2008
Fabrication of elastin-like polypeptide nanoparticles for drug delivery by electrospraying. Biomacromolecules\| PubMed ID: 19072041	2009
Peptide-based Biopolymers in Biomedicine and Biotechnology. Materials science & engineering. R, Reports : a review journal\| PubMed ID: 19122836	2008
Early metabolite levels predict long-term matrix accumulation for chondrocytes in elastin-like polypeptide biopolymer scaffolds. Tissue engineering. Part A\| PubMed ID: 19193139	2009
Rational selection of gold nanorod geometry for label-free plasmonic biosensors. ACS nano\| PubMed ID: 19296619	2009
Fusion order controls expression level and activity of elastin-like polypeptide fusion proteins. Protein science : a publication of the Protein Society\| PubMed ID: 19533768	2009
In situ growth of a stoichiometric PEG-like conjugate at a protein's N-terminus with significantly improved pharmacokinetics. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 19706892	2009
Neural network analysis identifies scaffold properties necessary for in vitro chondrogenesis in elastin-like polypeptide biopolymer scaffolds. Tissue engineering. Part A\| PubMed ID: 19754250	2010
Self-assembling chimeric polypeptide-doxorubicin conjugate nanoparticles that abolish tumours after a single injection. Nature materials\| PubMed ID: 19898461	2009
Hydrogen bonding of beta-turn structure is stabilized in D(2)O. Journal of the American Chemical Society\| PubMed ID: 19919159	2009
Elastin-like polypeptides: biomedical applications of tunable biopolymers. Biopolymers\| PubMed ID: 20091871	2010
Injectable intratumoral depot of thermally responsive polypeptide-radionuclide conjugates delays tumor progression in a mouse model. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 20117157	2010
Recursive directional ligation by plasmid reconstruction allows rapid and seamless cloning of oligomeric genes. Biomacromolecules\| PubMed ID: 20184309	2010
Morphing low-affinity ligands into high-avidity nanoparticles by thermally triggered self-assembly of a genetically encoded polymer. ACS nano\| PubMed ID: 20334355	2010
Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes. Langmuir : the ACS journal of surfaces and colloids\| PubMed ID: 20356046	2010
Applications of elastin-like polypeptides in tissue engineering. Advanced drug delivery reviews\| PubMed ID: 20385185	2010
Monitoring kinetics of surface initiated atom transfer radical polymerization by quartz crystal microbalance with dissipation. Biointerphases\| PubMed ID: 20408613	2006
Surface plasmon optical study of the interfacial phase transition of elastinlike polypeptide grafted on gold. Biointerphases\| PubMed ID: 20408702	2008
Biological applications of polymer brushes. Preface. Biointerphases\| PubMed ID: 20408712	2009
Versatile synthesis and micropatterning of nonfouling polymer brushes on the wafer scale. Biointerphases\| PubMed ID: 20408717	2009
Drug delivery to solid tumors by elastin-like polypeptides. Advanced drug delivery reviews\| PubMed ID: 20546809	2010
A versatile diffractive maskless lithography for single-shot and serial microfabrication. Optics express\| PubMed ID: 20589036	2010
Stimulus-responsive macromolecules and nanoparticles for cancer drug delivery. Nanomedicine (London, England)\| PubMed ID: 20662649	2010
Intracellular uptake and associated toxicity of silver nanoparticles in Caenorhabditis elegans. Aquatic toxicology (Amsterdam, Netherlands)\| PubMed ID: 20708279	2010
Leveraging nanoscale plasmonic modes to achieve reproducible enhancement of light. Nano letters\| PubMed ID: 20804206	2010
In situ growth of a PEG-like polymer from the C terminus of an intein fusion protein improves pharmacokinetics and tumor accumulation. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 20810920	2010
Elastin-like polypeptides as a purification tag for recombinant proteins. Current protocols in protein science / editorial board, John E. Coligan ... [et al.]\| PubMed ID: 20814933	2010
The use of micropatterning to control smooth muscle myosin heavy chain expression and limit the response to transforming growth factor β1 in vascular smooth muscle cells. Biomaterials\| PubMed ID: 20858564	2011
Quantitative Model of the Phase Behavior of Recombinant pH-Responsive Elastin-Like Polypeptides. Biomacromolecules\| PubMed ID: 20925333	2010
Chain stiffness of elastin-like polypeptides. Biomacromolecules\| PubMed ID: 20961120	2010
Gold nanoparticles on polarizable surfaces as Raman scattering antennas. ACS nano\| PubMed ID: 21038892	2010
Spatially addressable chemoselective C-terminal ligation of an intein fusion protein from a complex mixture to a hydrazine-terminated surface. Langmuir : the ACS journal of surfaces and colloids\| PubMed ID: 21142101	2011
Plasmonic flow cytometry by immunolabeled nanorods. Cytometry. Part A : the journal of the International Society for Analytical Cytology\| PubMed ID: 21182183	2011
A highly parallel method for synthesizing DNA repeats enables the discovery of 'smart' protein polymers. Nature materials\| PubMed ID: 21258353	2011
Micro- and nanostructured poly[oligo(ethylene glycol)methacrylate] brushes grown from photopatterned halogen initiators by atom transfer radical polymerization. Biointerphases\| PubMed ID: 21428690	2011
Self-assembly of monodisperse oligonucleotide-elastin block copolymers into stars and compound micelles. Chemistry (Weinheim an der Bergstrasse, Germany)\| PubMed ID: 21469235	2011
Amplified on-chip fluorescence detection of DNA hybridization by surface-initiated enzymatic polymerization. Analytical chemistry\| PubMed ID: 21604676	2011
In vivo tumor targeting by a NGR-decorated micelle of a recombinant diblock copolypeptide. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 21763734	2011
Dual-order snapshot spectral imaging of plasmonic nanoparticles. Applied optics\| PubMed ID: 21772408	2011
Field-induced nanolithography for patterning of non-fouling polymer brush surfaces. Small (Weinheim an der Bergstrasse, Germany)\| PubMed ID: 21901825	2011
Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles. Journal of biomedical optics\| PubMed ID: 22112108	2011
Fusions of elastin-like polypeptides to pharmaceutical proteins. Methods in enzymology\| PubMed ID: 22208987	2012
Triple stimulus-responsive polypeptide nanoparticles that enhance intratumoral spatial distribution. Nano letters\| PubMed ID: 22417133	2012
Doxorubicin-conjugated chimeric polypeptide nanoparticles that respond to mild hyperthermia. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 22421424	2012
Probing dynamically tunable localized surface plasmon resonances of film-coupled nanoparticles by evanescent wave excitation. Nano letters\| PubMed ID: 22429053	2012
Unexpected multivalent display of proteins by temperature triggered self-assembly of elastin-like polypeptide block copolymers. Biomacromolecules\| PubMed ID: 22515311	2012
Protein polymer hydrogels by in situ, rapid and reversible self-gelation. Biomaterials\| PubMed ID: 22538198	2012
Digital switching of local arginine density in a genetically encoded self-assembled polypeptide nanoparticle controls cellular uptake. Nano letters\| PubMed ID: 22625178	2012
Uptake of silver nanoparticles and toxicity to early life stages of Japanese medaka (Oryzias latipes): effect of coating materials. Aquatic toxicology (Amsterdam, Netherlands)\| PubMed ID: 22634717	2012
Rheological properties of cysteine-containing elastin-like polypeptide solutions and hydrogels. Biomacromolecules\| PubMed ID: 22789001	2012
Chemistry. Nanomaterials for drug delivery. Science (New York, N.Y.)\| PubMed ID: 22822138	2012
Overcoming limitations in nanoparticle drug delivery: triggered, intravascular release to improve drug penetration into tumors. Cancer research\| PubMed ID: 22952218	2012
Plasmon ruler with angstrom length resolution. ACS nano\| PubMed ID: 22966857	2012
Harnessing the power of cell-penetrating peptides: activatable carriers for targeting systemic delivery of cancer therapeutics and imaging agents. Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology\| PubMed ID: 22977001	2013
Fabrication of ssDNA/oligo(ethylene glycol) monolayers and complex nanostructures by an irradiation-promoted exchange reaction. Angewandte Chemie (International ed. in English)\| PubMed ID: 22987725	2012
Effect of detergents on the thermal behavior of elastin-like polypeptides. Biopolymers\| PubMed ID: 23097230	2013
Brachytherapy using injectable seeds that are self-assembled from genetically encoded polypeptides in situ. Cancer research\| PubMed ID: 23155121	2012
Direct fluorescence detection of RNA on microarrays by surface-initiated enzymatic polymerization. Analytical chemistry\| PubMed ID: 23194025	2013
Controlled-reflectance surfaces with film-coupled colloidal nanoantennas. Nature\| PubMed ID: 23222613	2012
Self-assembly of thermally responsive nanoparticles of a genetically encoded peptide polymer by drug conjugation. Angewandte Chemie (International ed. in English)\| PubMed ID: 23280697	2013
Injectable protease-operated depots of glucagon-like peptide-1 provide extended and tunable glucose control. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 23359691	2013
Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides. Angewandte Chemie (International ed. in English)\| PubMed ID: 23424160	2013
Predicting transition temperatures of elastin-like polypeptide fusion proteins. Biomacromolecules\| PubMed ID: 23565607	2013
Calcium binding peptide motifs from calmodulin confer divalent ion selectivity to elastin-like polypeptides. Biomacromolecules\| PubMed ID: 23705904	2013
A unified model for de novo design of elastin-like polypeptides with tunable inverse transition temperatures. Biomacromolecules\| PubMed ID: 23808597	2013
Hydration layer coupling and cooperativity in phase behavior of stimulus responsive peptide polymers. Journal of the American Chemical Society\| PubMed ID: 23822733	2013
A genetically engineered thermally responsive sustained release curcumin depot to treat neuroinflammation. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 23830979	2013
Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein. Macromolecular rapid communications\| PubMed ID: 23836349	2013
Spectroscopic study of a DNA brush synthesized in situ by surface initiated enzymatic polymerization. The journal of physical chemistry. B\| PubMed ID: 23899324	2013
Actively targeting solid tumours with thermoresponsive drug delivery systems that respond to mild hyperthermia. International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group\| PubMed ID: 23924317	2013
NANOSCALE SELF-ASSEMBLY FOR DELIVERY OF THERAPEUTICS AND IMAGING AGENTS. Technology and innovation\| PubMed ID: 24077873	2011
Plasmonic waveguide modes of film-coupled metallic nanocubes. Nano letters\| PubMed ID: 24199752	2013
"Smart" DNA interfaces. Chemical Society reviews\| PubMed ID: 24352168	2014
Enzymatic polymerization of high molecular weight DNA amphiphiles that self-assemble into star-like micelles. Advanced materials (Deerfield Beach, Fla.)\| PubMed ID: 24497034	2014
Controlled apoptosis by a thermally toggled nanoscale amplifier of cellular uptake. Nano letters\| PubMed ID: 24611762	2014
Rational design of "heat seeking" drug loaded polypeptide nanoparticles that thermally target solid tumors. Nano letters\| PubMed ID: 24738626	2014
Simple Assay for Proteases Based on Aggregation of Stimulus-Responsive Polypeptides. Analytical chemistry\| PubMed ID: 24832919	2014