Ashutosh Chilkoti

Ashutosh Chilkoti

Department of Biomedical Engineering, Duke University

Affiliated withDuke University

Research Area

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JoVE Journal Publications

ArticleTotal : 1
Year
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from <em>Escherichia coli</em>
Publication title

Cited by 48

2014

Other Publications

Article
Year
A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface.

Analytical chemistry| PubMed ID: 11838667

2002
2002
Targeted drug delivery by thermally responsive polymers.

Advanced drug delivery reviews| PubMed ID: 12204595

2002
2002
2002
2003
2003
2003
Evaluation of an elastin-like polypeptide-doxorubicin conjugate for cancer therapy.

Journal of controlled release : official journal of the Controlled Release Society| PubMed ID: 12932635

2003
2004
Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold.

Journal of the American Chemical Society| PubMed ID: 15080668

2004
2004
2004
2004
2004
2004
Label free colorimetric biosensing using nanoparticles.

Journal of fluorescence| PubMed ID: 15617380

2004
2006
Self-cleavable stimulus responsive tags for protein purification without chromatography.

Journal of the American Chemical Society| PubMed ID: 16089436

2005
2006
2005
2006
2005
Structural optimization of a "smart" doxorubicin-polypeptide conjugate for thermally targeted delivery to solid tumors.

Journal of controlled release : official journal of the Controlled Release Society| PubMed ID: 16303202

2006
2005
Tumor vascular permeability, accumulation, and penetration of macromolecular drug carriers.

Journal of the National Cancer Institute| PubMed ID: 16507830

2006
2006
2006
Purification of an elastin-like fusion protein by microfiltration.

Biotechnology and bioengineering| PubMed ID: 16767781

2006
2006
2006
Tracking the in vivo fate of recombinant polypeptides by isotopic labeling.

Journal of controlled release : official journal of the Controlled Release Society| PubMed ID: 16904221

2006
Tumor accumulation, degradation and pharmacokinetics of elastin-like polypeptides in nude mice.

Journal of controlled release : official journal of the Controlled Release Society| PubMed ID: 16919353

2006
A thermally responsive biopolymer for intra-articular drug delivery.

Journal of controlled release : official journal of the Controlled Release Society| PubMed ID: 16959360

2006
Stimulus responsive elastin biopolymers: Applications in medicine and biotechnology.

Current opinion in chemical biology| PubMed ID: 17055770

2006
2007
Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides.

Langmuir : the ACS journal of surfaces and colloids| PubMed ID: 17190524

2007
2007
2007
2007
2007
Force-induced prolyl cis-trans isomerization in elastin-like polypeptides.

Journal of the American Chemical Society| PubMed ID: 17469821

2007
2007
2007
2007
Toward a systems engineering approach to cancer drug delivery.

Journal of the National Cancer Institute| PubMed ID: 17596569

2007
2007
Surface-initiated enzymatic polymerization of DNA.

Langmuir : the ACS journal of surfaces and colloids| PubMed ID: 17929953

2007
2007
Temperature triggered self-assembly of polypeptides into multivalent spherical micelles.

Journal of the American Chemical Society| PubMed ID: 18085778

2008
2008
2008
2008
2008
Synthesis and characterization of a thermally-responsive tumor necrosis factor antagonist.

Journal of controlled release : official journal of the Controlled Release Society| PubMed ID: 18547669

2008
2008
Temperature sensitive peptides: engineering hyperthermia-directed therapeutics.

International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group| PubMed ID: 18608590

2008
Hydration and conformational mechanics of single, end-tethered elastin-like polypeptides.

Journal of the American Chemical Society| PubMed ID: 18646848

2008
2008
2008
2009
Peptide-based Biopolymers in Biomedicine and Biotechnology.

Materials science & engineering. R, Reports : a review journal| PubMed ID: 19122836

2008
2009
2009
Fusion order controls expression level and activity of elastin-like polypeptide fusion proteins.

Protein science : a publication of the Protein Society| PubMed ID: 19533768

2009
In situ growth of a stoichiometric PEG-like conjugate at a protein's N-terminus with significantly improved pharmacokinetics.

Proceedings of the National Academy of Sciences of the United States of America| PubMed ID: 19706892

2009
2010
2009
Hydrogen bonding of beta-turn structure is stabilized in D(2)O.

Journal of the American Chemical Society| PubMed ID: 19919159

2009
2010
Injectable intratumoral depot of thermally responsive polypeptide-radionuclide conjugates delays tumor progression in a mouse model.

Journal of controlled release : official journal of the Controlled Release Society| PubMed ID: 20117157

2010
2010
2010
Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes.

Langmuir : the ACS journal of surfaces and colloids| PubMed ID: 20356046

2010
Applications of elastin-like polypeptides in tissue engineering.

Advanced drug delivery reviews| PubMed ID: 20385185

2010
2006
2008
Biological applications of polymer brushes. Preface.

Biointerphases| PubMed ID: 20408712

2009
2009
Drug delivery to solid tumors by elastin-like polypeptides.

Advanced drug delivery reviews| PubMed ID: 20546809

2010
2010
2010
Intracellular uptake and associated toxicity of silver nanoparticles in Caenorhabditis elegans.

Aquatic toxicology (Amsterdam, Netherlands)| PubMed ID: 20708279

2010
2010
In situ growth of a PEG-like polymer from the C terminus of an intein fusion protein improves pharmacokinetics and tumor accumulation.

Proceedings of the National Academy of Sciences of the United States of America| PubMed ID: 20810920

2010
Elastin-like polypeptides as a purification tag for recombinant proteins.

Current protocols in protein science / editorial board, John E. Coligan ... [et al.]| PubMed ID: 20814933

2010
2011
2010
Chain stiffness of elastin-like polypeptides.

Biomacromolecules| PubMed ID: 20961120

2010
2010
2011
Plasmonic flow cytometry by immunolabeled nanorods.

Cytometry. Part A : the journal of the International Society for Analytical Cytology| PubMed ID: 21182183

2011
2011
2011
2011
2011
In vivo tumor targeting by a NGR-decorated micelle of a recombinant diblock copolypeptide.

Journal of controlled release : official journal of the Controlled Release Society| PubMed ID: 21763734

2011
2011
Field-induced nanolithography for patterning of non-fouling polymer brush surfaces.

Small (Weinheim an der Bergstrasse, Germany)| PubMed ID: 21901825

2011
2011
2012
2012
Doxorubicin-conjugated chimeric polypeptide nanoparticles that respond to mild hyperthermia.

Journal of controlled release : official journal of the Controlled Release Society| PubMed ID: 22421424

2012
2012
2012
2012
2012
2012
2012
Chemistry. Nanomaterials for drug delivery.

Science (New York, N.Y.)| PubMed ID: 22822138

2012
2012
2012
2013
2012
2013
2012
2013
2012
2013
Injectable protease-operated depots of glucagon-like peptide-1 provide extended and tunable glucose control.

Proceedings of the National Academy of Sciences of the United States of America| PubMed ID: 23359691

2013
2013
2013
2013
2013
2013
A genetically engineered thermally responsive sustained release curcumin depot to treat neuroinflammation.

Journal of controlled release : official journal of the Controlled Release Society| PubMed ID: 23830979

2013
2013
2013
Actively targeting solid tumours with thermoresponsive drug delivery systems that respond to mild hyperthermia.

International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group| PubMed ID: 23924317

2013
2011
2013
"Smart" DNA interfaces.

Chemical Society reviews| PubMed ID: 24352168

2014
2014
2014
2014
2014