preparation-light-responsive-membranes-combined-surface-grafting

Preparation of Light-responsive Membranes by a Combined Surface Grafting and Postmodification Process

In order to modify the surface tension of commercial available track-edged polymer membranes, a procedure of surface-initiated polymerization is ...

Empa, Swiss Federal Laboratories for Materials Science and Technology

Yeast two-hybrid based systems are powerful tools for the detection and characterization of protein-protein interactions (PPIs). However, some important protein classes, e.g., integral membrane proteins and transcription factors, are difficult to study using these technologies. To overcome these limitations, we have employed a novel protein complementation screening platform. Protein interactions are detected by reconstitution of the split-protein sensor TRP1, enabling trp1 cells to grow on medium lacking tryptophan. Since the interaction readout is direct and independent of transcriptional reporter activation the rate of false positives is lowered. Furthermore, the technology allows for detection of protein interactions in their natural setting, e.g., the cytosol, the nucleus, and at cellular or organellar membranes. The protocols used for screening are explained in detail and as an example we describe the isolation of novel binding partners found with APP screened against a human cDNA library.

Application of the split-protein sensor Trp1 to protein interaction discovery in the yeast Saccharomyces cerevisiae.

Light-responsive membranes based on a porous polycarbonate (PC) matrix were developed by surface functionalization with spirobenzopyran (SP)-containing polymers. The surface modification was generated by plasma-induced surface graft polymerization. Mass transfer rates of caffeine through these membranes were found to be up to eight times higher under UV irradiation than at daylight.

Article	Year
Application of the split-protein sensor Trp1 to protein interaction discovery in the yeast Saccharomyces cerevisiae. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 22218864	2012
Light-responsive caffeine transfer through porous polycarbonate. ACS applied materials & interfaces\| PubMed ID: 23790045	2013

Lukas Baumann

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