radio-frequency-identification-motion-sensitive-video-efficiently

Radio Frequency Identification and Motion-sensitive Video Efficiently Automate Recording of Unrewarded Choice Behavior by Bumblebees

We present two methods for observing bumblebee choice behavior in an enclosed testing space. The first method consists of Radio Frequency Identification ...

University of Ottawa

We presented bumblebees a spatial memory task similar to that used with other species (e.g., cats, dogs, and pigeons). In some conditions we allowed for presence of scent marks in addition to placing local and global spatial cues in conflict. Bumblebees (Bombus impatiens) were presented an array of artificial flowers within a flight cage, one flower offering reward (S+), while the others were empty (S-). Bees were tested with empty flowers. In Experiment 1, flowers were either moved at the time of testing or not. Bees returned to the flower in the same absolute position of the S+ (the flower-array-independent (FAI) position), even if it was in the wrong position relative to the S- and even when new flower covers prevented the use of possible scent marks. New flower covers (i.e., without possible scent marks) had the effect of lowering the frequency of probing behavior. In Experiment 2, the colony was moved between training and testing. Again, bees chose the flower in the FAI position of the S+, and not the flower that would be chosen using strictly memory for a flight vector. Together, these experiments show that to locate the S+ bees did not rely on scent marks nor the positions of the S-, though the S- were prominent objects close to the goal. Also, bees selected environmental features to remember the position of the S+ instead of relying upon a purely egocentric point of view. Similarities with honeybees and vertebrates are discussed, as well as possible encoding mechanisms.

Spatial encoding by bumblebees (Bombus impatiens) of a reward within an artificial flower array.

How do distinct visual stimuli help bumblebees discover flowers before they have experienced any reward outside of their nest? Two visual floral properties, type of a pattern (concentric vs radial) and its position on unrewarding artificial flowers (central vs peripheral on corolla), were manipulated in two experiments. Both visual properties showed significant effects on floral choice. When pitted against each other, pattern was more important than position. Experiment 1 shows a significant effect of concentric pattern position, and experiment 2 shows a significant preference towards radial patterns regardless of their position. These results show that the presence of markings at the center of a flower are not so important as the presence of markings that will direct bees there.

The effect of flower-like and non-flower-like visual properties on choice of unrewarding patterns by bumblebees.

Studies of bee cognition frequently use two-dimensional stimuli referred to as floral patterns, and yet how bees perceive pictorial representations is not known. An investigation of bumblebee (Bombus impatiens) picture-object correspondence was undertaken according to the theory of Fagot et al. (Picture perception in animals. Psychology Press Ltd, East Sussex, pp 295-320, 2000) that pictures and objects may be confused, perceived as independent or equivalent. In three experiments, bumblebees were given discrimination training and unrewarded testing in a radial maze. In the first experiment, preferences between artificial flowers and photographs of those flowers revealed a significant learned preference for the stimulus rewarded during training: no confusion following training. In the second experiment, bees did transfer learning from artificial flowers to photographs: some commonality between an object and photograph was perceived. In the third experiment, bees spontaneously generalized a learned preference for one artificial flower to its silhouette, but only for one of two flowers used in training. No generalization was obtained to drawn images. Some transfer between image and object is exhibited, likely by low-level feature matching, but transfer is poor with degraded images, cautioning against extrapolation of picture-based responding to natural correspondents. Despite evidence that bees exhibit some transfer while retaining discrimination, it is likely that the observed response is due to generalization more akin to confusion than true equivalence. Furthermore, although 2D patterning cues (line, edge and shade) provide discriminable cues for bees between 2D stimuli, it is not here supported that such features are perceived as equivalent to the intended floral structures.

Article	Year
Spatial encoding by bumblebees (Bombus impatiens) of a reward within an artificial flower array. Animal cognition\| PubMed ID: 16416106	2006
The effect of flower-like and non-flower-like visual properties on choice of unrewarding patterns by bumblebees. Die Naturwissenschaften\| PubMed ID: 23771705	2013
How images may or may not represent flowers: picture-object correspondence in bumblebees (Bombus impatiens)? Animal cognition\| PubMed ID: 24531905	2014

Catherine M.S. Plowright

Biography

JoVE Journal Publications

Other Publications