Raymond J. Owens

Oxford Protein Production Facility, Research Complex at Harwell

Affiliated withResearch Complex at Harwell

Research Area

Biography

Raymond J. Owens has not added Biography.
If you are Raymond J. Owens and would like to personalize this page please email our Author Liaison for assistance.

JoVE Journal Publications

ArticleTotal : 1	Year
Publication title Cited by 34	2015

Other Publications

Article	Year
Crystal structure of nitrogen regulatory protein IIANtr from Neisseria meningitidis. BMC structural biology\| PubMed ID: 16092953	2005
Structure of the PII signal transduction protein of Neisseria meningitidis at 1.85 A resolution. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 16754965	2006
Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE. Acta crystallographica. Section D, Biological crystallography\| PubMed ID: 17001088	2006
Lysine methylation as a routine rescue strategy for protein crystallization. Structure (London, England : 1993)\| PubMed ID: 17098187	2006
Analysis of variable N-glycosylation site occupancy in glycoproteins by liquid chromatography electrospray ionization mass spectrometry. Analytical biochemistry\| PubMed ID: 17178093	2007
A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Nucleic acids research\| PubMed ID: 17317681	2007
Glycoprotein structural genomics: solving the glycosylation problem. Structure (London, England : 1993)\| PubMed ID: 17355862	2007
The structure and transcriptional analysis of a global regulator from Neisseria meningitidis. The Journal of biological chemistry\| PubMed ID: 17374605	2007
Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues. The Journal of general virology\| PubMed ID: 17622627	2007
Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 Angstrom resolution. Protein science : a publication of the Protein Society\| PubMed ID: 17893366	2007
Semi-automated microseeding of nanolitre crystallization experiments. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 18097093	2008
Protein production and purification. Nature methods\| PubMed ID: 18235434	2008
Structure of the cold-shock domain protein from Neisseria meningitidis reveals a strand-exchanged dimer. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 18391418	2008
Expression, purification and crystallization of a lyssavirus matrix (M) protein. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 18391421	2008
Structures of an alanine racemase from Bacillus anthracis (BA0252) in the presence and absence of (R)-1-aminoethylphosphonic acid (L-Ala-P). Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 18453697	2008
The crystal structure of UMP kinase from Bacillus anthracis (BA1797) reveals an allosteric nucleotide-binding site. Journal of molecular biology\| PubMed ID: 18625239	2008
A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells. Protein expression and purification\| PubMed ID: 18662785	2008
Vaccinia virus proteins A52 and B14 Share a Bcl-2-like fold but have evolved to inhibit NF-kappaB rather than apoptosis. PLoS pathogens\| PubMed ID: 18704168	2008
Crystallization and preliminary X-ray analysis of CrgA, a LysR-type transcriptional regulator from pathogenic Neisseria meningitidis MC58. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 18765907	2008
Generation of baculovirus vectors for the high-throughput production of proteins in insect cells. Biotechnology and bioengineering\| PubMed ID: 18781697	2008
The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 18988019	2009
The production of glycoproteins by transient expression in Mammalian cells. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 18988030	2009
Rhabdovirus matrix protein structures reveal a novel mode of self-association. PLoS pathogens\| PubMed ID: 19112510	2008
The structure of NMB1585, a MarR-family regulator from Neisseria meningitidis. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 19255465	2009
The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators. Nucleic acids research\| PubMed ID: 19474343	2009
Crystallization and preliminary X-ray analysis of mouse RANK and its complex with RANKL. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 19478440	2009
Genetic modification of a baculovirus vector for increased expression in insect cells. Cell biology and toxicology\| PubMed ID: 19655260	2010
Crystal structure of a novel conformational state of the flavivirus NS3 protein: implications for polyprotein processing and viral replication. Journal of virology\| PubMed ID: 19793813	2009
Structural plasticity of eph receptor A4 facilitates cross-class ephrin signaling. Structure (London, England : 1993)\| PubMed ID: 19836338	2009
Structure and functionality in flavivirus NS-proteins: perspectives for drug design. Antiviral research\| PubMed ID: 19945487	2010
Recent advances in the production of proteins in insect and mammalian cells for structural biology. Journal of structural biology\| PubMed ID: 20153433	2010
The crystal structure of NGO0477 from Neisseria gonorrhoeae reveals a novel protein fold incorporating a helix-turn-helix motif. Proteins\| PubMed ID: 20196080	2010
Improved expression of secreted and membrane-targeted proteins in insect cells. Biotechnology and applied biochemistry\| PubMed ID: 20441568	2010
The structure of a reduced form of OxyR from Neisseria meningitidis. BMC structural biology\| PubMed ID: 20478059	2010
Structural and functional insights of RANKL-RANK interaction and signaling. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 20483727	2010
Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains. BMC structural biology\| PubMed ID: 20509910	2010
Crystal structures of penicillin-binding protein 3 from Pseudomonas aeruginosa: comparison of native and antibiotic-bound forms. Journal of molecular biology\| PubMed ID: 20974151	2011
Expression of protein complexes using multiple Escherichia coli protein co-expression systems: a benchmarking study. Journal of structural biology\| PubMed ID: 21382497	2011
xtalPiMS: a PiMS-based web application for the management and monitoring of crystallization trials. Journal of structural biology\| PubMed ID: 21605683	2011
Converting monoclonal antibodies into Fab fragments for transient expression in mammalian cells. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 21987252	2012
Structure of New Delhi metallo-β-lactamase 1 (NDM-1). Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 22102018	2011
Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development. PloS one\| PubMed ID: 22174749	2011
In situ macromolecular crystallography using microbeams. Acta crystallographica. Section D, Biological crystallography\| PubMed ID: 22525757	2012
Structure of the regulatory domain of the LysR family regulator NMB2055 (MetR-like protein) from Neisseria meningitidis. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 22750853	2012
Outrunning free radicals in room-temperature macromolecular crystallography. Acta crystallographica. Section D, Biological crystallography\| PubMed ID: 22751666	2012
Structural and functional characterization of the kindlin-1 pleckstrin homology domain. The Journal of biological chemistry\| PubMed ID: 23132860	2012
Structure of ribose 5-phosphate isomerase from the probiotic bacterium Lactobacillus salivarius UCC118. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 23192019	2012
Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology. Current opinion in structural biology\| PubMed ID: 23623336	2013
S-Adenosyl-S-carboxymethyl-L-homocysteine: a novel cofactor found in the putative tRNA-modifying enzyme CmoA. Acta crystallographica. Section D, Biological crystallography\| PubMed ID: 23695253	2013
Crystal structure of signal regulatory protein gamma (SIRPγ) in complex with an antibody Fab fragment. BMC structural biology\| PubMed ID: 23826770	2013
Assay platform for clinically relevant metallo-β-lactamases. Journal of medicinal chemistry\| PubMed ID: 23898798	2013
Binding of (5S)-penicilloic acid to penicillin binding protein 3. ACS chemical biology\| PubMed ID: 23899657	2013
A streamlined implementation of the glutamine synthetase-based protein expression system. BMC biotechnology\| PubMed ID: 24063773	2013
Chromophore-linked substrate (CLS405): probing metallo-β-lactamase activity and inhibition. ChemMedChem\| PubMed ID: 24166830	2013
Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 24395367	2014
Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. Protein engineering, design & selection : PEDS\| PubMed ID: 24668773	2014
Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 25447866	2015
GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 25502201	2015