leprdb-mouse-model-type-2-diabetes-pancreatic-islet-isolation-live

Lepr<sup>db</sup> Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

Type 2 diabetes is a chronic disease affecting 382 million people in 2013, and is expected to rise to 592 million by 2035 1. During the past 2 decades, ...

The University of Queensland

In dispersed single beta cells the response of each cell to glucose is heterogeneous. In contrast, within an islet, cell-to-cell communication leads to glucose inducing a more homogeneous response. For example, increases in NAD(P)H and calcium are relatively uniform across the cells of the islet. These data suggest that secretion of insulin from single beta cells within an islet should also be relatively homogeneous. The aim of this study was to test this hypothesis by determining the glucose dependence of single-cell insulin responses within an islet.

Glucose principally regulates insulin secretion in mouse islets by controlling the numbers of granule fusion events per cell.

We used the db/db mouse to determine the nature of the secretory defect in intact islets.

Article	Year
Glucose principally regulates insulin secretion in mouse islets by controlling the numbers of granule fusion events per cell. Diabetologia\| PubMed ID: 23995471	2013
The secretory deficit in islets from db/db mice is mainly due to a loss of responding beta cells. Diabetologia\| PubMed ID: 24705605	2014

Oanh H. Do

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