Michael Karas

Department of Pharmaceutical Chemistry, Goethe University of Frankfurt

Affiliated withGoethe University of Frankfurt

Research Area

Biography

Michael Karas has not added Biography.
If you are Michael Karas and would like to personalize this page please email our Author Liaison for assistance.

JoVE Journal Publications

ArticleTotal : 1	Year
Publication title Cited by 9	2015

Other Publications

Article	Year
Carbon Source-dependent assembly of the Snf1p kinase complex in Candida albicans. The Journal of biological chemistry\| PubMed ID: 15890650	2005
Comparative proteomic analysis of the rat spinal cord in inflammatory and neuropathic pain models. Neuroscience letters\| PubMed ID: 15896486	2005
Mass spectrometric sequencing of individual peptides from combinatorial libraries via specific generation of chain-terminated sequences. Journal of combinatorial chemistry\| PubMed ID: 11831885	2002
Identification of anhydroecgonine methyl ester N-oxide, a new metabolite of anhydroecgonine methyl ester, using electrospray mass spectrometry. Journal of mass spectrometry : JMS\| PubMed ID: 12112758	2002
Formation and fate of ion pairs during MALDI analysis: anion adduct generation as an indicative tool to determine ionization processes. Journal of the American Society for Mass Spectrometry\| PubMed ID: 12387328	2002
Structural analysis of underivatized neutral human milk oligosaccharides in the negative ion mode by nano-electrospray MS(n) (part 1: methodology). Journal of the American Society for Mass Spectrometry\| PubMed ID: 12443024	2002
Structural analysis of underivatized neutral human milk oligosaccharides in the negative ion mode by nano-electrospray MS(n) (part 2: application to isomeric mixtures). Journal of the American Society for Mass Spectrometry\| PubMed ID: 12443025	2002
Ion formation in MALDI: the cluster ionization mechanism. Chemical reviews\| PubMed ID: 12580637	2003
Activation of subtilin precursors by Bacillus subtilis extracellular serine proteases subtilisin (AprE), WprA, and Vpr. Biochemical and biophysical research communications\| PubMed ID: 12705882	2003
Effect of different solution flow rates on analyte ion signals in nano-ESI MS, or: when does ESI turn into nano-ESI? Journal of the American Society for Mass Spectrometry\| PubMed ID: 12745218	2003
The cyclin-dependent kinase inhibitor p21CIP/WAF is a positive regulator of insulin-like growth factor I-induced cell proliferation in MCF-7 human breast cancer cells. The Journal of biological chemistry\| PubMed ID: 12867429	2003
Degradation of ginsenosides in humans after oral administration. Drug metabolism and disposition: the biological fate of chemicals\| PubMed ID: 12867496	2003
Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation. Journal of lipid research\| PubMed ID: 12923235	2003
Determination of hyperforin in mouse brain by high-performance liquid chromatography/tandem mass spectrometry. Analytical chemistry\| PubMed ID: 14615985	2003
Multiple signaling pathways are involved in the regulation of IGF-I receptor inhibition of PTEN-enhanced apoptosis. Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society\| PubMed ID: 14700555	2004
Calibration of ion effective temperatures achieved by resonant activation in a quadrupole ion trap. Analytical chemistry\| PubMed ID: 14708790	2003
PTEN inhibits cell proliferation and induces apoptosis by downregulating cell surface IGF-IR expression in prostate cancer cells. Oncogene\| PubMed ID: 14737113	2004
Effects of organ-specific loss of insulin-like growth factor-I production on murine hematopoiesis. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation\| PubMed ID: 14752777	2004
TDAG51 mediates the effects of insulin-like growth factor I (IGF-I) on cell survival. The Journal of biological chemistry\| PubMed ID: 15037619	2004
14-3-3sigma mediation of cell cycle progression is p53-independent in response to insulin-like growth factor-I receptor activation. The Journal of biological chemistry\| PubMed ID: 15187095	2004
Physicochemical characterisation of cationic polybutylcyanoacrylate-nanoparticles by fluorescence correlation spectroscopy. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V\| PubMed ID: 15207534	2004
Mass spectrometric mapping of the enzymes involved in the phenol degradation of an indigenous soil pseudomonad. Biochimica et biophysica acta\| PubMed ID: 15210131	2004
CD8+ cytotoxic T lymphocytes isolated from allogeneic healthy donors recognize HLA class Ia/Ib-associated renal carcinoma antigens with ubiquitous or restricted tissue expression. Blood\| PubMed ID: 15231579	2004
Internal energy build-up in matrix-assisted laser desorption/ionization. Journal of mass spectrometry : JMS\| PubMed ID: 15236295	2004
Insulin-like growth factor-I stimulates both cell growth and lipogenesis during differentiation of human mesenchymal stem cells into adipocytes. The Journal of clinical endocrinology and metabolism\| PubMed ID: 15240644	2004
Processing of the 24 kDa subunit mitochondrial import signal is not required for assembly of functional complex I in Yarrowia lipolytica. European journal of biochemistry / FEBS\| PubMed ID: 15317595	2004
Hyperforin modifies neuronal membrane properties in vivo. Neuroscience letters\| PubMed ID: 15331138	2004
Two-dimensional electrophoresis for the isolation of integral membrane proteins and mass spectrometric identification. Proteomics\| PubMed ID: 15352231	2004
Chronic administration of statins alters multiple gene expression patterns in mouse cerebral cortex. The Journal of pharmacology and experimental therapeutics\| PubMed ID: 15358814	2005
Membrane proteome analysis of the green-sulfur bacterium Chlorobium tepidum. Electrophoresis\| PubMed ID: 15490440	2004
Detection and characterisation of catechol 2,3-dioxygenase in an indigenous soil pseudomonad by MALDI-TOF MS using a column separation. Biodegradation\| PubMed ID: 15730028	2005
Lipid rafts associate with intracellular B cell receptors and exhibit a B cell stage-specific protein composition. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 15749887	2005
Use of two-dimensional gel electrophoresis in predictive toxicology: identification of potential early protein biomarkers in chemically induced hepatocarcinogenesis. Proteomics\| PubMed ID: 15816005	2005
A four-subunit cytochrome bc(1) complex complements the respiratory chain of Thermus thermophilus. Biochimica et biophysica acta\| PubMed ID: 15869739	2005
Molecular weights of poly(butyl cyanoacrylate) nanoparticles determined by mass spectrometry and size exclusion chromatography. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V\| PubMed ID: 15996580	2005
Rapid and sensitive identification of major histocompatibility complex class I-associated tumor peptides by Nano-LC MALDI MS/MS. Molecular & cellular proteomics : MCP\| PubMed ID: 16112985	2005
LC-nanospray-MS/MS analysis of hydrophobic proteins from membrane protein complexes isolated by blue-native electrophoresis. Journal of mass spectrometry : JMS\| PubMed ID: 16127664	2005
Immunoisolation of two synaptic vesicle pools from synaptosomes: a proteomics analysis. Journal of neurochemistry\| PubMed ID: 16269012	2005
Proteomic analysis of chlorosome-depleted membranes of the green sulfur bacterium Chlorobium tepidum. Proteomics\| PubMed ID: 16294302	2006
Collision-induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap-orbitrap mass spectrometry... Journal of mass spectrometry : JMS\| PubMed ID: 16498600	2006
High throughput two-dimensional blue-native electrophoresis: a tool for functional proteomics of cytoplasmatic protein complexes from Chlorobium tepidum. Photosynthesis research\| PubMed ID: 16622784	2006
Functional characterization of BbCRASP-2, a distinct outer membrane protein of Borrelia burgdorferi that binds host complement regulators factor H and FHL-1. Molecular microbiology\| PubMed ID: 16925556	2006
Synaptic vesicle proteins under conditions of rest and activation: analysis by 2-D difference gel electrophoresis. Electrophoresis\| PubMed ID: 16944461	2006
Mass spectrometric analysis of glycine receptor-associated gephyrin splice variants. The Journal of biological chemistry\| PubMed ID: 17001074	2006
Biochemical and electron microscopic characterization of the F1F0 ATP synthase from the hyperthermophilic eubacterium Aquifex aeolicus. FEBS letters\| PubMed ID: 17045990	2006
Analysis of the synaptic vesicle proteome using three gel-based protein separation techniques. Proteomics\| PubMed ID: 17080482	2006
Mechanism of substrate sensing and signal transmission within an ABC transporter: use of a Trojan horse strategy. The Journal of biological chemistry\| PubMed ID: 17164240	2007
An alternative strategy for the membrane proteome analysis of the green sulfur bacterium Chlorobium tepidum using blue native PAGE and 2-D PAGE on purified membranes. Journal of proteome research\| PubMed ID: 17261056	2007
High resolution clear native electrophoresis for in-gel functional assays and fluorescence studies of membrane protein complexes. Molecular & cellular proteomics : MCP\| PubMed ID: 17426019	2007
Prevalidation of potential protein biomarkers in toxicology using iTRAQ reagent technology. Proteomics\| PubMed ID: 17443845	2007
A dedicated haem lyase is required for the maturation of a novel bacterial cytochrome c with unconventional covalent haem binding. Molecular microbiology\| PubMed ID: 17501927	2007
Identification of two proteins associated with mammalian ATP synthase. Molecular & cellular proteomics : MCP\| PubMed ID: 17575325	2007
Investigation of sample-purification procedures for MALDI-based proteomic studies. Analytical and bioanalytical chemistry\| PubMed ID: 17668194	2007
Scaffold hopping by "fuzzy" pharmacophores and its application to RNA targets. Chembiochem : a European journal of chemical biology\| PubMed ID: 17896338	2007
Components of the translational machinery are associated with juvenile glycine receptors and are redistributed to the cytoskeleton upon aging and synaptic activity. The Journal of biological chemistry\| PubMed ID: 17965018	2007
The key residue for substrate transport (Glu14) in the EmrE dimer is asymmetric. The Journal of biological chemistry\| PubMed ID: 18042544	2008
Metabolism of boswellic acids in vitro and in vivo. Drug metabolism and disposition: the biological fate of chemicals\| PubMed ID: 18356270	2008
Identification of the mitochondrial ND3 subunit as a structural component involved in the active/deactive enzyme transition of respiratory complex I. The Journal of biological chemistry\| PubMed ID: 18502755	2008
Using fluorescence dyes as a tool for analyzing the MALDI process. Journal of the American Society for Mass Spectrometry\| PubMed ID: 18513990	2008
Detection and relative quantification of siRNA double strands by MALDI mass spectrometry. Analytical chemistry\| PubMed ID: 18616347	2008
Differential phosphoproteome profiling reveals a functional role for VASP in Helicobacter pylori-induced cytoskeleton turnover in gastric epithelial cells. Cellular microbiology\| PubMed ID: 18637808	2008
Comparison of the membrane subproteomes during growth of a new pseudomonas strain on lysogeny broth medium, glucose, and phenol. Journal of proteome research\| PubMed ID: 18707154	2008
4-Chloro-alpha-cyanocinnamic acid is an advanced, rationally designed MALDI matrix. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 18723668	2008
Elastase digests: new ammunition for shotgun membrane proteomics. Molecular & cellular proteomics : MCP\| PubMed ID: 19116210	2009
The proteome of the presynaptic active zone: from docked synaptic vesicles to adhesion molecules and maxi-channels. Journal of neurochemistry\| PubMed ID: 19187093	2009
The new matrix 4-chloro-alpha-cyanocinnamic acid allows the detection of phosphatidylethanolamine chloramines by MALDI-TOF mass spectrometry. Journal of the American Society for Mass Spectrometry\| PubMed ID: 19201617	2009
Sequencing of single and double stranded RNA oligonucleotides by acid hydrolysis and MALDI mass spectrometry. Analytical chemistry\| PubMed ID: 19296685	2009
Comparison between vacuum sublimed matrices and conventional dried droplet preparation in MALDI-TOF mass spectrometry. Journal of the American Society for Mass Spectrometry\| PubMed ID: 19299165	2009
Comparison between the matrices alpha-cyano-4-hydroxycinnamic acid and 4-chloro-alpha-cyanocinnamic acid for trypsin, chymotrypsin, and pepsin digestions by MALDI-TOF mass spectrometry. Journal of proteome research\| PubMed ID: 19435303	2009
Nitric oxide-independent vasodilator rescues heme-oxidized soluble guanylate cyclase from proteasomal degradation. Circulation research\| PubMed ID: 19478201	2009
Identification of human cathepsin G as a functional target of boswellic acids from the anti-inflammatory remedy frankincense. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 19648270	2009
Quantitative site-specific analysis of protein glycosylation by LC-MS using different glycopeptide-enrichment strategies. Analytical biochemistry\| PubMed ID: 19699707	2009
The benefit of combining nLC-MALDI-Orbitrap MS data with nLC-MALDI-TOF/TOF data for proteomic analyses employing elastase. Journal of proteome research\| PubMed ID: 19725589	2009
Metabotropic glutamate receptor 4 interacts with microtubule-associated protein 1B. Biochemical and biophysical research communications\| PubMed ID: 19781524	2009
Rapid simultaneous quantitative determination of different small pharmaceutical drugs using a conventional matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system... Rapid communications in mass spectrometry : RCM\| PubMed ID: 19844965	2009
Highly specific capture and direct MALDI MS analysis of phosphopeptides by zirconium phosphonate on self-assembled monolayers. Analytical chemistry\| PubMed ID: 19968246	2010
Membrane protein analysis using an improved peptic in-solution digestion protocol. Proteomics\| PubMed ID: 20017156	2009
The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase. Nucleic acids research\| PubMed ID: 20047967	2010
Quantitative determination of acetylcholine and choline in microdialysis samples by MALDI-TOF MS. Analytical chemistry\| PubMed ID: 20058877	2010
Approaching the complexity of elastase-digested membrane proteomes using off-gel IEF/nLC-MALDI-MS/MS. Analytical chemistry\| PubMed ID: 20136094	2010
A novel polyacrylamide gel system for proteomic use offering controllable pore expansion by crosslinker cleavage. Electrophoresis\| PubMed ID: 20162583	2010
Mass estimation of native proteins by blue native electrophoresis: principles and practical hints. Molecular & cellular proteomics : MCP\| PubMed ID: 20173216	2010
Assembly and oligomerization of human ATP synthase lacking mitochondrial subunits a and A6L. Biochimica et biophysica acta\| PubMed ID: 20188060	2010
Enhanced glyco-profiling by specific glycopeptide enrichment and complementary monolithic nano-LC (ZIC-HILIC/RP18e)/ESI-MS analysis. Journal of separation science\| PubMed ID: 20222078	2010
Membrane proteome of the green sulfur bacterium Chlorobium tepidum (syn. Chlorobaculum tepidum) analyzed by gel-based and gel-free methods. Photosynthesis research\| PubMed ID: 20349210	2010
Peptide mass fingerprinting after less specific in-gel proteolysis using MALDI-LTQ-Orbitrap and 4-chloro-alpha-cyanocinnamic acid. Journal of proteome research\| PubMed ID: 20364821	2010
3-Aminoquinoline acting as matrix and derivatizing agent for MALDI MS analysis of oligosaccharides. Analytical chemistry\| PubMed ID: 20387804	2010
Analysis of boronic acids by nano liquid chromatography-direct electron ionization mass spectrometry. Analytical chemistry\| PubMed ID: 20411924	2010
Significant sensitivity improvements by matrix optimization: a MALDI-TOF mass spectrometric study of lipids from hen egg yolk. Chemistry and physics of lipids\| PubMed ID: 20420816	2010
100% protein sequence coverage: a modern form of surrealism in proteomics. Amino acids\| PubMed ID: 20625782	2011
Identification of novel human adipocyte secreted proteins by using SGBS cells. Journal of proteome research\| PubMed ID: 20681635	2010
Hypoxia and reoxygenation of primary human hepatocytes induce proteome changes of glucose metabolism, oxidative protection and peroxisomal function. International journal of molecular medicine\| PubMed ID: 20818499	2010
Labeling elastase digests with TMT: informational gain by identification of poorly detectable peptides with MALDI-TOF/TOF mass spectrometry. Proteomics\| PubMed ID: 20925058	2010
The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Î¨1191 in yeast 18S rRNA. Nucleic acids research\| PubMed ID: 20972225	2011
Complement factor H-related proteins CFHR2 and CFHR5 represent novel ligands for the infection-associated CRASP proteins of Borrelia burgdorferi. PloS one\| PubMed ID: 20975954	2010
Entianin, a novel subtilin-like lantibiotic from Bacillus subtilis subsp. spizizenii DSM 15029T with high antimicrobial activity. Applied and environmental microbiology\| PubMed ID: 21239550	2011
Proteome profiling suggests a pro-inflammatory role for plasma cells through release of high-mobility group box 1 protein. Proteomics\| PubMed ID: 21319304	2011
A high-definition native polyacrylamide gel electrophoresis system for the analysis of membrane complexes. The Plant journal : for cell and molecular biology\| PubMed ID: 21418111	2011
Structure elucidation and biosynthesis of lysine-rich cyclic peptides in Xenorhabdus nematophila. Organic & biomolecular chemistry\| PubMed ID: 21423922	2011
Simple dual-spotting procedure enhances nLC-MALDI MS/MS analysis of digests with less specific enzymes. Journal of proteome research\| PubMed ID: 21480673	2011
A proteomic approach to investigate the differential antigenic profile of two Coxiella burnetii strains. Journal of proteomics\| PubMed ID: 21565289	2011
Nerve injury evoked loss of latexin expression in spinal cord neurons contributes to the development of neuropathic pain. PloS one\| PubMed ID: 21572518	2011
Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: lipidome meets proteome. Biochimica et biophysica acta\| PubMed ID: 21820081	2011
The trafficking proteins Vacuolar Protein Sorting 35 and Neurobeachin interact with the glycine receptor Î²-subunit. Biochemical and biophysical research communications\| PubMed ID: 21821005	2011
Fast quantitative determination of melamine and its derivatives by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid communications in mass spectrometry : RCM\| PubMed ID: 21913263	2011
High-reactivity matrices increase the sensitivity of matrix enhanced secondary ion mass spectrometry. Analytical chemistry\| PubMed ID: 21939214	2011
Compelling evidence for Lucky Survivor and gas phase protonation: the unified MALDI analyte protonation mechanism. Journal of the American Society for Mass Spectrometry\| PubMed ID: 21953039	2011
Nano-liquid chromatography-direct electron ionization mass spectrometry: improving performance by a new ion source adapter. Journal of mass spectrometry : JMS\| PubMed ID: 22012666	2011
Inhibition of 5-lipoxygenase by U73122 is due to covalent binding to cysteine 416. Biochimica et biophysica acta\| PubMed ID: 22137889	2012
Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs. RNA (New York, N.Y.)\| PubMed ID: 22274954	2012
Heme-copper terminal oxidase using both cytochrome c and ubiquinol as electron donors. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 22334648	2012
Perfusion reversed-phase high-performance liquid chromatography for protein separation from detergent-containing solutions: an alternative to gel-based approaches. Analytical biochemistry\| PubMed ID: 22370273	2012
Premature transcript termination, trans-splicing and DNA repair: a vicious path to cancer. American journal of blood research\| PubMed ID: 22432062	2011
Improving the precision of quantitative bottom-up proteomics based on stable isotope-labeled proteins. Analytical and bioanalytical chemistry\| PubMed ID: 22535440	2012
Ion yields in UV-MALDI mass spectrometry as a function of excitation laser wavelength and optical and physico-chemical properties of classical and halogen-substituted MALDI matrixes. Analytical chemistry\| PubMed ID: 22803742	2012
Neutral loss fragmentation pattern based screening for arginine-rich natural products in Xenorhabdus and Photorhabdus. Analytical chemistry\| PubMed ID: 22873683	2012
Graphite supported preparation (GSP) of Î±-cyano-4-hydroxycinnamic acid (CHCA) for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for peptides and proteins. Journal of the American Society for Mass Spectrometry\| PubMed ID: 22993043	2012
Lipidome and proteome of lipid droplets from the methylotrophic yeast Pichia pastoris. Biochimica et biophysica acta\| PubMed ID: 23041514	2013
Enzyme-cleavable tandem peptides for quantitative studies in MS-based proteomics. Proteomics\| PubMed ID: 23090848	2012
Comparative analysis of barley leaf proteome as affected by drought stress. Planta\| PubMed ID: 23129216	2013
Defining the core proteome of the chloroplast envelope membranes. Frontiers in plant science\| PubMed ID: 23390424	2013
TEMPORARY REMOVAL: Adsorption of plasma proteins on uncoated PLGA nanoparticles. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V\| PubMed ID: 23395970	2013
Mass spectrometric detection of multiple extended series of neutral highly fucosylated N-acetyllactosamine oligosaccharides in human milk. International journal of mass spectrometry\| PubMed ID: 23538872	2008
MALDI analysis of proteins after extraction from dissolvable ethylene glycol diacrylate cross-linked polyacrylamide gels. Electrophoresis\| PubMed ID: 23775326	2013
Enhanced MALDI MS sensitivity by weak base additives and glycerol sample coating. Analytical chemistry\| PubMed ID: 24325339	2014
Sulfo-NHS-SS-biotin derivatization: a versatile tool for MALDI mass analysis of PTMs in lysine-rich proteins. Proteomics\| PubMed ID: 24449390	2014
The proteome of the presynaptic active zone from mouse brain. Molecular and cellular neurosciences\| PubMed ID: 24534009	2014
Analysis of 5-lipoxygenase phosphorylation on molecular level by MALDI-MS. The FEBS journal\| PubMed ID: 24860872	2014
PADI4 acts as a coactivator of Tal1 by counteracting repressive histone arginine methylation. Nature communications\| PubMed ID: 24874575	2014
Secretome analysis of Anabaena sp. PCC 7120 and the involvement of the TolC-homologue HgdD in protein secretion. Environmental microbiology\| PubMed ID: 24890022	2015
Self-built microdialysis probes with improved recoveries of ATP and neuropeptides. Journal of neuroscience methods\| PubMed ID: 25172804	2014
Comparative Analysis of Sorghum bicolor Proteome in Response to Drought Stress and following Recovery. International journal of proteomics\| PubMed ID: 25349737	2014
The association of late-acting snoRNPs with human pre-ribosomal complexes requires the RNA helicase DDX21. Nucleic acids research\| PubMed ID: 25477391	2015
Characterization of common and distinctive adjustments of wild barley leaf proteome under drought acclimation, heat stress and their combination. Plant molecular biology\| PubMed ID: 25647426	2015