the-mouse-round-window-approach-for-ototoxic-agent-delivery-rapid

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

Investigators have utilized a wide array of animal models and investigative techniques to study the mammalian auditory system. Much of the basic research ...

Intuitive Surgical, Inc

To evaluate whether irreversible electroporation (IRE) has the potential to damage nerves in a porcine model and to compare histopathologic findings after IRE with histopathologic findings after radiofrequency ablation (RFA).

Acute and subacute effects of irreversible electroporation on nerves: experimental study in a pig model.

To evaluate the effects of irreversible electroporation (IRE) on the rectum wall after IRE applied adjacent to the rectum.

Irreversible electroporation adjacent to the rectum: evaluation of pathological effects in a pig model.

To evaluate the delayed effects of irreversible electroporation (IRE) ablation on nerves.

The delayed effects of irreversible electroporation ablation on nerves.

Ibuprofen, a nonsteroidal antiinflammatory drug, is a nonselective inhibitor of cyclooxygenases 1 and 2 that commonly is used for its analgesic, antiinflammatory, and antipyretic properties. We compared the palatability and efficacy of medicated water containing ibuprofen from an oral pediatric suspension or liqui-gel capsules in C57BL/6 and genetically engineered mice of C57BL/6 background with ulcerative dermatitis. Mice (n = 14 or 15 per group) with ulcerated skin lesions of similar average size (capsule group, 6.71 mm(2); suspension group, 6.12 mm(2)) received ibuprofen in their drinking water at a concentration of 1 mg/mL. Water and food consumption, locomotor activity, grooming frequency, and reduction in pruritic behavior and lesion size were measured over a 9-d period. Compared with those treated with water containing the suspension, mice that received medicated water containing the liqui-gel formulation drank more (mean, 6.8 compared with 11.7 mL/d), consumed more food (4.02 compared with 2.73 g/d), and showed less pruritic behavior, greater healing (mean, 29.3% compare with 64.8%), and more locomotor activity over a 9-d period.

Palatability and treatment efficacy of various ibuprofen formulations in C57BL/6 mice with ulcerative dermatitis.

The purpose of this study was to assess the relationship between size and the continuity of energy application in interstitial laser-induced thermotherapy. Percutaneous computed tomography-guided laser ablation (30 W, 600 nm diode) of the lung was performed in 7 Yorkshire pigs; a total of 42 ablation zones were created. Twenty ablations were performed using a continuous cycle of 2 min (protocol A) and 22 ablations were performed using 4 intermittent cycles with a duration of 1 min for each cycle interrupted by a 10-s stop between the cycles (protocol B). The lung was harvested immediately after euthanasia for gross pathology and histopathologic evaluation. Statistical analysis was performed using the Student t test and the Spearman correlation coefficient. Laser ablation resulted in complete necrosis of variable size of lung. The mean ablation zone dimensions (±SD) were 1.9 (±0.4) cm × 1.4 (±0.3) cm for protocol A and 2.2 (±0.5) cm × 1.4 (±0.4) cm for protocol B. The size of the necrosis is not significantly different when comparing a continuous 2-min ablation to a 4-cycle intermittent ablation for 1 min each cycle interrupted by a 10-s stop between the cycles (P = 0.98 and 0.53, respectively).

Interstitial laser-induced thermotherapy of the lung: evaluation of the influence of ablation continuity on ablation size in a swine model.

To study the feasibility of a novel endorectal electrode for the creation of focal ablations of the rectal wall with the use of irreversible electroporation (IRE).

Evaluation of an endorectal electrode for performing focused irreversible electroporation ablations in the Swine rectum.

To investigate the reliability of simulations for planning pancreatic irreversible electroporation (IRE) ablations compared with computed tomography (CT) and pathology outcomes in an animal model.

Comparison of simulation-based treatment planning with imaging and pathology outcomes for percutaneous CT-guided irreversible electroporation of the porcine pancreas: a pilot study.

High-intensity focused ultrasound (HIFU) is a noninvasive thermal ablation technique. High-intensity focused ultrasound has been used in small-animal models to lesion neural tissue selectively. This study aimed to evaluate the efficacy of HIFU in a large-animal model for ablation of nerves similar in size to human nerves.

Novel use of noninvasive high-intensity focused ultrasonography for intercostal nerve neurolysis in a swine model.

Image-guided thermal ablation is used to treat primary and secondary liver cancers. Evaluating completeness of ablation is difficult with standard intraoperative B-mode ultrasound. This study evaluates the ability of B-mode ultrasound (US) and tissue elastography to adequately measure the extent of ablation compared to pathologic assessment.

Intraoperative ultrasound and tissue elastography measurements do not predict the size of hepatic microwave ablations.

Numerical simulations are used for treatment planning in clinical applications of irreversible electroporation (IRE) to determine ablation size and shape. To assess the reliability of simulations for treatment planning, we compared simulation results with empiric outcomes of renal IRE using computed tomography (CT) and histology in an animal model.

Planning irreversible electroporation in the porcine kidney: are numerical simulations reliable for predicting empiric ablation outcomes?

Spastic patients often seek neurolysis, the permanent destruction of the sciatic nerve, for better pain management. MRI-guided high-intensity focused ultrasound (MRgHIFU) may serve as a noninvasive alternative to the prevailing, more intrusive techniques. This in vivo acute study is aimed at performing sciatic nerve neurolysis using a clinical MRgHIFU system.

Article	Year
Acute and subacute effects of irreversible electroporation on nerves: experimental study in a pig model. Radiology\| PubMed ID: 21642418	2011
Irreversible electroporation adjacent to the rectum: evaluation of pathological effects in a pig model. Cardiovascular and interventional radiology\| PubMed ID: 22562481	2013
The delayed effects of irreversible electroporation ablation on nerves. European radiology\| PubMed ID: 23011210	2013
Palatability and treatment efficacy of various ibuprofen formulations in C57BL/6 mice with ulcerative dermatitis. Journal of the American Association for Laboratory Animal Science : JAALAS\| PubMed ID: 23312090	2012
Interstitial laser-induced thermotherapy of the lung: evaluation of the influence of ablation continuity on ablation size in a swine model. Cancer imaging : the official publication of the International Cancer Imaging Society\| PubMed ID: 23439016	2013
Evaluation of an endorectal electrode for performing focused irreversible electroporation ablations in the Swine rectum. Journal of vascular and interventional radiology : JVIR\| PubMed ID: 23796856	2013
Comparison of simulation-based treatment planning with imaging and pathology outcomes for percutaneous CT-guided irreversible electroporation of the porcine pancreas: a pilot study. Journal of vascular and interventional radiology : JVIR\| PubMed ID: 23891044	2013
Novel use of noninvasive high-intensity focused ultrasonography for intercostal nerve neurolysis in a swine model. Regional anesthesia and pain medicine\| PubMed ID: 24317231	2014
Intraoperative ultrasound and tissue elastography measurements do not predict the size of hepatic microwave ablations. Academic radiology\| PubMed ID: 24331267	2014
Planning irreversible electroporation in the porcine kidney: are numerical simulations reliable for predicting empiric ablation outcomes? Cardiovascular and interventional radiology\| PubMed ID: 24831827	2015
Feasibility Study on MR-Guided High-Intensity Focused Ultrasound Ablation of Sciatic Nerve in a Swine Model: Preliminary Results. Cardiovascular and interventional radiology\| PubMed ID: 26040256	2015

Paula C. Ezell

Biography

JoVE Journal Publications

Other Publications