Michael E. Himmel

BioEnergy Science Center, National Renewable Energy Laboratory

Affiliated withNational Renewable Energy Laboratory National Renewable Energy Laboratory

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JoVE Journal Publications

ArticleTotal : 1	Year
Publication title Cited by 19	2015

Other Publications

Article	Year
Exploration of cellulose surface-binding properties of acidothermus cellulolyticus Cel5A by site-specific mutagenesis. Applied biochemistry and biotechnology\| PubMed ID: 12018255	2002
Effect of single active-site cleft mutation on product specificity in a thermostable bacterial cellulase. Applied biochemistry and biotechnology\| PubMed ID: 12018266	2002
Automated filter paper assay for determination of cellulase activity. Applied biochemistry and biotechnology\| PubMed ID: 12721448	2003
The bacterial scaffoldin: structure, function and potential applications in the nanosciences. Genetic engineering\| PubMed ID: 15260240	2003
Direct imaging of surface-enhanced Raman scattering in the near field. Langmuir : the ACS journal of surfaces and colloids\| PubMed ID: 15641817	2005
Catalytically enhanced endocellulase Cel5A from Acidothermus cellulolyticus. Applied biochemistry and biotechnology\| PubMed ID: 15917594	2005
Acidic sugar degradation pathways: an ab initio molecular dynamics study. Applied biochemistry and biotechnology\| PubMed ID: 15930576	2005
Electrochemical oxidation of water by a cellobiose dehydrogenase from Phanerochaete chrysosporium. Biotechnology letters\| PubMed ID: 15973489	2005
Ab initio molecular dynamics simulations of beta-D-glucose and beta-D-xylose degradation mechanisms in acidic aqueous solution. Carbohydrate research\| PubMed ID: 16095579	2005
Self-assembly of photoactive TiO2-cyclodextrin wires. Journal of the American Chemical Society\| PubMed ID: 16248603	2005
Beta-D-glucosidase reaction kinetics from isothermal titration microcalorimetry. Analytical biochemistry\| PubMed ID: 16269126	2005
Computer simulation studies of microcrystalline cellulose Ibeta. Carbohydrate research\| PubMed ID: 16297893	2006
The maize primary cell wall microfibril: a new model derived from direct visualization. Journal of agricultural and food chemistry\| PubMed ID: 16448156	2006
Determination of lignin by size exclusion chromatography using multi angle laser light scattering. Journal of chromatography. A\| PubMed ID: 16566937	2006
Mechanisms of glycerol dehydration. The journal of physical chemistry. A\| PubMed ID: 16671686	2006
Outlook for cellulase improvement: screening and selection strategies. Biotechnology advances\| PubMed ID: 16690241	2006
Catalyst transport in corn stover internodes: elucidating transport mechanisms using Direct Blue-I. Applied biochemistry and biotechnology\| PubMed ID: 16915666	2006
Energetics of xylose decomposition as determined using quantum mechanics modeling. The journal of physical chemistry. A\| PubMed ID: 17048814	2006
Versatile derivatives of carbohydrate-binding modules for imaging of complex carbohydrates approaching the molecular level of resolution. BioTechniques\| PubMed ID: 17068959	2006
Expression and characterization of Acidothermus cellulolyticus E1 endoglucanase in transgenic duckweed Lemna minor 8627. Bioresource technology\| PubMed ID: 17127051	2007
Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science (New York, N.Y.)\| PubMed ID: 17289988	2007
Fractionating recalcitrant lignocellulose at modest reaction conditions. Biotechnology and bioengineering\| PubMed ID: 17318910	2007
Cellulase digestibility of pretreated biomass is limited by cellulose accessibility. Biotechnology and bioengineering\| PubMed ID: 17335064	2007
Microscopic analysis of corn fiber using starch- and cellulose-specific molecular probes. Biotechnology and bioengineering\| PubMed ID: 17335065	2007
Molecular modeling suggests induced fit of Family I carbohydrate-binding modules with a broken-chain cellulose surface. Protein engineering, design & selection : PEDS\| PubMed ID: 17430975	2007
The potential of cellulases and cellulosomes for cellulosic waste management. Current opinion in biotechnology\| PubMed ID: 17462879	2007
Deposition of lignin droplets produced during dilute acid pretreatment of maize stems retards enzymatic hydrolysis of cellulose. Biotechnology progress\| PubMed ID: 17973399	2007
Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities. Bioresource technology\| PubMed ID: 18006303	2008
Heterologous expression of Aspergillus niger beta-D-xylosidase (XlnD): characterization on lignocellulosic substrates. Applied biochemistry and biotechnology\| PubMed ID: 18421587	2008
Heterologous expression of two ferulic acid esterases from Penicillium funiculosum. Applied biochemistry and biotechnology\| PubMed ID: 18421589	2008
Implications of cellobiohydrolase glycosylation for use in biomass conversion. Biotechnology for biofuels\| PubMed ID: 18471276	2008
Welcome to biotechnology for biofuels. Biotechnology for biofuels\| PubMed ID: 18471312	2008
A biophysical perspective on the cellulosome: new opportunities for biomass conversion. Current opinion in biotechnology\| PubMed ID: 18513939	2008
Heterologous expression of glycosyl hydrolases in planta: a new departure for biofuels. Trends in biotechnology\| PubMed ID: 18579242	2008
Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment. Biotechnology and bioengineering\| PubMed ID: 18781690	2008
Redistribution of xylan in maize cell walls during dilute acid pretreatment. Biotechnology and bioengineering\| PubMed ID: 19161247	2009
Engineered carbohydrate-binding module (CBM) protein-suspended single-walled carbon nanotubes in water. Chemical communications (Cambridge, England)\| PubMed ID: 19209321	2009
The effect of lignin removal by alkaline peroxide pretreatment on the susceptibility of corn stover to purified cellulolytic and xylanolytic enzymes. Applied biochemistry and biotechnology\| PubMed ID: 19214798	2009
Detecting cellulase penetration into corn stover cell walls by immuno-electron microscopy. Biotechnology and bioengineering\| PubMed ID: 19266575	2009
Perspectives and new directions for the production of bioethanol using consolidated bioprocessing of lignocellulose. Current opinion in biotechnology\| PubMed ID: 19520566	2009
Lignocellulose conversion to biofuels: current challenges, global perspectives. Current opinion in biotechnology\| PubMed ID: 19523813	2009
The effects of water on beta-D-xylose condensation reactions. The journal of physical chemistry. A\| PubMed ID: 19572686	2009
The energy landscape for the interaction of the family 1 carbohydrate-binding module and the cellulose surface is altered by hydrolyzed glycosidic bonds. The journal of physical chemistry. B\| PubMed ID: 19594145	2009
Computational simulations of the Trichoderma reesei cellobiohydrolase I acting on microcrystalline cellulose Ibeta: the enzyme-substrate complex. Carbohydrate research\| PubMed ID: 19699474	2009
Plant cell wall characterization using scanning probe microscopy techniques. Biotechnology for biofuels\| PubMed ID: 19703302	2009
Identification of amino acids responsible for processivity in a Family 1 carbohydrate-binding module from a fungal cellulase. The journal of physical chemistry. B\| PubMed ID: 20050714	2010
Glucose reversion reaction kinetics. Journal of agricultural and food chemistry\| PubMed ID: 20429509	2010
Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance. Biotechnology for biofuels\| PubMed ID: 20497524	2010
Label-free, real-time monitoring of biomass processing with stimulated Raman scattering microscopy. Angewandte Chemie (International ed. in English)\| PubMed ID: 20589818	2010
The unique binding mode of cellulosomal CBM4 from Clostridium thermocellum cellobiohydrolase A. Journal of molecular biology\| PubMed ID: 20654622	2010
The role of hydrogen-bonding interactions in acidic sugar reaction pathways. Carbohydrate research\| PubMed ID: 20667524	2010
Structure of a fibronectin type III-like module from Clostridium thermocellum. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 20693658	2010
Simulation studies of the insolubility of cellulose. Carbohydrate research\| PubMed ID: 20705283	2010
Antisense down-regulation of 4CL expression alters lignification, tree growth, and saccharification potential of field-grown poplar. Plant physiology\| PubMed ID: 20729393	2010
Sequencing of multiple clostridial genomes related to biomass conversion and biofuel production. Journal of bacteriology\| PubMed ID: 20889752	2010
Free energy landscape for glucose condensation reactions. The journal of physical chemistry. A\| PubMed ID: 21086968	2010
Modeling the self-assembly of the cellulosome enzyme complex. The Journal of biological chemistry\| PubMed ID: 21098021	2011
The O-glycosylated linker from the Trichoderma reesei Family 7 cellulase is a flexible, disordered protein. Biophysical journal\| PubMed ID: 21112302	2010
In situ imaging of single carbohydrate-binding modules on cellulose microfibrils. The journal of physical chemistry. B\| PubMed ID: 21162585	2011
Applications of computational science for understanding enzymatic deconstruction of cellulose. Current opinion in biotechnology\| PubMed ID: 21168322	2011
In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize. Biotechnology for biofuels\| PubMed ID: 21269444	2011
Cellobiohydrolase hydrolyzes crystalline cellulose on hydrophobic faces. The Journal of biological chemistry\| PubMed ID: 21282110	2011
High throughput determination of glucan and xylan fractions in lignocelluloses. Biotechnology letters\| PubMed ID: 21287235	2011
High-temperature behavior of cellulose I. The journal of physical chemistry. B\| PubMed ID: 21338135	2011
Sugar-binding sites on the surface of the carbohydrate-binding module of CBH I from Trichoderma reesei. Carbohydrate research\| PubMed ID: 21377658	2011
Molecular-level origins of biomass recalcitrance: decrystallization free energies for four common cellulose polymorphs. The journal of physical chemistry. B\| PubMed ID: 21425804	2011
Probing carbohydrate product expulsion from a processive cellulase with multiple absolute binding free energy methods. The Journal of biological chemistry\| PubMed ID: 21454590	2011
Structure of CBM4 from Clostridium thermocellum cellulase K. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 21543854	2011
Bioprospecting metagenomics of decaying wood: mining for new glycoside hydrolases. Biotechnology for biofuels\| PubMed ID: 21816041	2011
Protein allostery at the solid-liquid interface: endoglucanase attachment to cellulose affects glucan clenching in the binding cleft. Journal of the American Chemical Society\| PubMed ID: 21877736	2011
Multiple functions of aromatic-carbohydrate interactions in a processive cellulase examined with molecular simulation. The Journal of biological chemistry\| PubMed ID: 21965672	2011
Effects of alkaline or liquid-ammonia treatment on crystalline cellulose: changes in crystalline structure and effects on enzymatic digestibility. Biotechnology for biofuels\| PubMed ID: 22011342	2011
Elucidating the role of ferrous ion cocatalyst in enhancing dilute acid pretreatment of lignocellulosic biomass. Biotechnology for biofuels\| PubMed ID: 22074910	2011
Weakly hydrated surfaces and the binding interactions of small biological solutes. European biophysics journal : EBJ\| PubMed ID: 22124617	2012
A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 22139151	2011
Computational investigation of glycosylation effects on a family 1 carbohydrate-binding module. The Journal of biological chemistry\| PubMed ID: 22147693	2012
Harnessing glycosylation to improve cellulase activity. Current opinion in biotechnology\| PubMed ID: 22186222	2012
Molecular dynamics simulations of the interaction of glucose with imidazole in aqueous solution. Carbohydrate research\| PubMed ID: 22248861	2012
Structure and function of the Clostridium thermocellum cellobiohydrolase A X1-module repeat: enhancement through stabilization of the CbhA complex. Acta crystallographica. Section D, Biological crystallography\| PubMed ID: 22349231	2012
Deconstruction of lignocellulosic biomass to fuels and chemicals. Annual review of chemical and biomolecular engineering\| PubMed ID: 22432613	2011
Tracking dynamics of plant biomass composting by changes in substrate structure, microbial community, and enzyme activity. Biotechnology for biofuels\| PubMed ID: 22490508	2012
Binding preferences, surface attachment, diffusivity, and orientation of a family 1 carbohydrate-binding module on cellulose. The Journal of biological chemistry\| PubMed ID: 22496371	2012
The metagenome of an anaerobic microbial community decomposing poplar wood chips. PloS one\| PubMed ID: 22629327	2012
Product binding varies dramatically between processive and nonprocessive cellulase enzymes. The Journal of biological chemistry\| PubMed ID: 22648408	2012
Challenges for assessing the performance of biomass degrading biocatalysts. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 22843384	2012
Coherent Raman microscopy analysis of plant cell walls. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 22843388	2012
Single-molecule tracking of carbohydrate-binding modules on cellulose using fluorescence microscopy. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 22843396	2012
Analysis of transgenic glycoside hydrolases expressed in plants: T. reesei CBH I and A. cellulolyticus EI. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 22843401	2012
Improved ethanol yield and reduced Minimum Ethanol Selling Price (MESP) by modifying low severity dilute acid pretreatment with deacetylation and mechanical refining: 1) Experimental. Biotechnology for biofuels\| PubMed ID: 22888758	2012
Considering water availability and the effect of solute concentration on high solids saccharification of lignocellulosic biomass. Biotechnology progress\| PubMed ID: 22915470	2012
Improved ethanol yield and reduced minimum ethanol selling price (MESP) by modifying low severity dilute acid pretreatment with deacetylation and mechanical refining: 2) Techno-economic anal... Biotechnology for biofuels\| PubMed ID: 22967479	2012
Sequence, structure, and evolution of cellulases in glycoside hydrolase family 48. The Journal of biological chemistry\| PubMed ID: 23055526	2012
Paradigmatic status of an endo- and exoglucanase and its effect on crystalline cellulose degradation. Biotechnology for biofuels\| PubMed ID: 23095278	2012
Cellulase linkers are optimized based on domain type and function: insights from sequence analysis, biophysical measurements, and molecular simulation. PloS one\| PubMed ID: 23139804	2012
How does plant cell wall nanoscale architecture correlate with enzymatic digestibility? Science (New York, N.Y.)\| PubMed ID: 23180856	2012
Computational investigation of the pH dependence of loop flexibility and catalytic function in glycoside hydrolases. The Journal of biological chemistry\| PubMed ID: 23504310	2013
The structure and mode of action of Caldicellulosiruptor bescii family 3 pectate lyase in biomass deconstruction. Acta crystallographica. Section D, Biological crystallography\| PubMed ID: 23519661	2013
Crystal structure and computational characterization of the lytic polysaccharide monooxygenase GH61D from the Basidiomycota fungus Phanerochaete chrysosporium. The Journal of biological chemistry\| PubMed ID: 23525113	2013
Binding site dynamics and aromatic-carbohydrate interactions in processive and non-processive family 7 glycoside hydrolases. The journal of physical chemistry. B\| PubMed ID: 23534900	2013
Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 23733951	2013
Hydration and saccharification of cellulose Iβ, II and III(I) at increasing dry solids loadings. Biotechnology letters\| PubMed ID: 23881312	2013
Glycosylated linkers in multimodular lignocellulose-degrading enzymes dynamically bind to cellulose. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 23959893	2013
Endoglucanase peripheral loops facilitate complexation of glucan chains on cellulose via adaptive coupling to the emergent substrate structures. The journal of physical chemistry. B\| PubMed ID: 23972069	2013
Improving activity of minicellulosomes by integration of intra- and intermolecular synergies. Biotechnology for biofuels\| PubMed ID: 23987588	2013
3D electron tomography of pretreated biomass informs atomic modeling of cellulose microfibrils. ACS nano\| PubMed ID: 23988022	2013
Genomic, proteomic, and biochemical analyses of oleaginous Mucor circinelloides: evaluating its capability in utilizing cellulolytic substrates for lipid production. PloS one\| PubMed ID: 24023719	2013
Impact of alg3 gene deletion on growth, development, pigment production, protein secretion, and functions of recombinant Trichoderma reesei cellobiohydrolases in Aspergillus niger. Fungal genetics and biology : FG & B\| PubMed ID: 24076077	2013
Cel48A from Thermobifida fusca: structure and site directed mutagenesis of key residues. Biotechnology and bioengineering\| PubMed ID: 24264519	2014
Glycoside hydrolase processivity is directly related to oligosaccharide binding free energy. Journal of the American Chemical Society\| PubMed ID: 24279927	2013
Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 Å resolution. Acta crystallographica. Section F, Structural biology and crystallization communications\| PubMed ID: 24316824	2013
Revealing nature's cellulase diversity: the digestion mechanism of Caldicellulosiruptor bescii CelA. Science (New York, N.Y.)\| PubMed ID: 24357319	2013
The complete mitochondrial genome of Limnoria quadripunctata Holthuis (Isopoda: Limnoriidae). Mitochondrial DNA\| PubMed ID: 24409843	2014
Multiscale deconstruction of molecular architecture in corn stover. Scientific reports\| PubMed ID: 24441444	2014
Experimental and modeling studies of an unusual water-filled pore structure with possible mechanistic implications in family 48 cellulases. The journal of physical chemistry. B\| PubMed ID: 24471470	2014
Charge engineering of cellulases improves ionic liquid tolerance and reduces lignin inhibition. Biotechnology and bioengineering\| PubMed ID: 24522957	2014
Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 2: morphological and structural substrate analysis. Biotechnology for biofuels\| PubMed ID: 24690534	2014
Agave proves to be a low recalcitrant lignocellulosic feedstock for biofuels production on semi-arid lands. Biotechnology for biofuels\| PubMed ID: 24708685	2014
Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 1: chemical and physical substrate analysis. Biotechnology for biofuels\| PubMed ID: 24713111	2014
Comparison of transcriptional profiles of Clostridium thermocellum grown on cellobiose and pretreated yellow poplar using RNA-Seq. Frontiers in microbiology\| PubMed ID: 24782837	2014
Response to Comment on "Revealing nature's cellulase diversity: the digestion mechanism of Caldicellulosiruptor bescii CelA". Science (New York, N.Y.)\| PubMed ID: 24812382	2014
Specificity of O-glycosylation in enhancing the stability and cellulose binding affinity of Family 1 carbohydrate-binding modules. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 24821760	2014
Towards a molecular-level theory of carbohydrate processivity in glycoside hydrolases. Current opinion in biotechnology\| PubMed ID: 24863902	2014
Carbohydrate-protein interactions that drive processive polysaccharide translocation in enzymes revealed from a computational study of cellobiohydrolase processivity. Journal of the American Chemical Society\| PubMed ID: 24869982	2014
Predicting enzyme adsorption to lignin films by calculating enzyme surface hydrophobicity. The Journal of biological chemistry\| PubMed ID: 24876380	2014
The structure of the catalytic domain of a plant cellulose synthase and its assembly into dimers. The Plant cell\| PubMed ID: 25012190	2014
NIR and Py-mbms coupled with multivariate data analysis as a high-throughput biomass characterization technique: a review. Frontiers in plant science\| PubMed ID: 25147552	2014
Deletion of Caldicellulosiruptor bescii CelA reveals its crucial role in the deconstruction of lignocellulosic biomass. Biotechnology for biofuels\| PubMed ID: 25317205	2014
Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing. Biotechnology for biofuels\| PubMed ID: 25337149	2014
Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica. PloS one\| PubMed ID: 25462572	2014
Heterologous protein expression in Hypocrea jecorina: a historical perspective and new developments. Biotechnology advances\| PubMed ID: 25479282	2015
High temperature pre-digestion of corn stover biomass for improved product yields. Biotechnology for biofuels\| PubMed ID: 25489338	2014
Fungal cellulases. Chemical reviews\| PubMed ID: 25629559	2015
Discrete and structurally unique proteins (tāpirins) mediate attachment of extremely thermophilic Caldicellulosiruptor species to cellulose. The Journal of biological chemistry\| PubMed ID: 25720489	2015
Homologous expression of the Caldicellulosiruptor bescii CelA reveals that the extracellular protein is glycosylated. PloS one\| PubMed ID: 25799047	2015
Annual acknowledgement of manuscript reviewers. Biotechnology for biofuels\| PubMed ID: 25821517	2015
The molecular origins of twist in cellulose I-beta. Carbohydrate polymers\| PubMed ID: 25857969	2015
A constitutive expression system for glycosyl hydrolase family 7 cellobiohydrolases in Hypocrea jecorina. Biotechnology for biofuels\| PubMed ID: 25904982	2015
Cofactor Specificity of the Bifunctional Alcohol and Aldehyde Dehydrogenase (AdhE) in Wild-Type and Mutant Clostridium thermocellum and Thermoanaerobacterium saccharolyticum. Journal of bacteriology\| PubMed ID: 26013492	2015
Identifying the ionically bound cell wall and intracellular glycoside hydrolases in late growth stage Arabidopsis stems: implications for the genetic engineering of bioenergy crops. Frontiers in plant science\| PubMed ID: 26029221	2015
Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose. Biotechnology for biofuels\| PubMed ID: 26269712	2015
In situ micro-spectroscopic investigation of lignin in poplar cell walls pretreated by maleic acid. Biotechnology for biofuels\| PubMed ID: 26312066	2015
The catalytic mechanism and unique low pH optimum of Caldicellulosiruptor bescii family 3 pectate lyase. Acta crystallographica. Section D, Biological crystallography\| PubMed ID: 26327384	2015