Keiji Numata

Enzyme Research Team, RIKEN Center for Sustainable Resource Science

Affiliated withRIKEN Center for Sustainable Resource Science

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JoVE Journal Publications

ArticleTotal : 1	Year
Publication title Cited by 14	2016

Other Publications

Article	Year
In-situ atomic force microscopy observation of enzymatic degradation in poly(hydroxyalkanoic acid) thin films: normal and constrained conditions. Macromolecular bioscience\| PubMed ID: 15468218	2004
Enzymatic degradation processes of lamellar crystals in thin films for poly[(R)-3-hydroxybutyric acid] and its copolymers revealed by real-time atomic force microscopy. Biomacromolecules\| PubMed ID: 15530032	2004
Biosynthesis of polyhydroxyalkanoate (PHA) copolymer from fructose using wild-type and laboratory-evolved PHA synthases. Macromolecular bioscience\| PubMed ID: 15729719	2005
Enzymatic degradation processes of poly[(R)-3-hydroxybutyric acid] and poly[(R)-3-hydroxybutyric acid-co-(R)-3-hydroxyvaleric acid] single crystals revealed by atomic force microscopy: effec... Biomacromolecules\| PubMed ID: 16004439	2005
Adsorption of biopolyester depolymerase on silicon wafer and poly[(R)-3-hydroxybutyric acid] single crystal revealed by real-time AFM. Macromolecular bioscience\| PubMed ID: 16374769	2006
Nonspecific hydrophobic interactions of a repressor protein, PhaR, with poly[(R)-3-hydroxybutyrate] film studied with a quartz crystal microbalance. Biomacromolecules\| PubMed ID: 16903695	2006
Adsorption and hydrolysis reactions of poly(hydroxybutyric acid) depolymerases secreted from Ralstonia pickettii T1 and Penicillium funiculosum onto poly[(R)-3-hydroxybutyric acid]. Biomacromolecules\| PubMed ID: 17547455	2007
Branched poly(lactide) synthesized by enzymatic polymerization: effects of molecular branches and stereochemistry on enzymatic degradation and alkaline hydrolysis. Biomacromolecules\| PubMed ID: 17722879	2007
Enzymatic degradation of monolayer for poly(lactide) revealed by real-time atomic force microscopy: effects of stereochemical structure, molecular weight, and molecular branches on hydrolysi... Biomacromolecules\| PubMed ID: 18636774	2008
Bioengineered silk protein-based gene delivery systems. Biomaterials\| PubMed ID: 19577803	2009
Expression, cross-linking, and characterization of recombinant chitin binding resilin. Biomacromolecules\| PubMed ID: 19928816	2009
Mechanism of enzymatic degradation of beta-sheet crystals. Biomaterials\| PubMed ID: 20044136	2010
Reinforcing silk scaffolds with silk particles. Macromolecular bioscience\| PubMed ID: 20166230	2010
Mechanisms of enzymatic degradation of amyloid Beta microfibrils generating nanofilaments and nanospheres related to cytotoxicity. Biochemistry\| PubMed ID: 20196618	2010
Surface-patterned electrode bioreactor for electrical stimulation. Lab on a chip\| PubMed ID: 20221556	2010
Silk-based delivery systems of bioactive molecules. Advanced drug delivery reviews\| PubMed ID: 20298729	2010
Gene delivery mediated by recombinant silk proteins containing cationic and cell binding motifs. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 20457191	2010
Silk-Based Gene Carriers with Cell Membrane Destabilizing Peptides. Biomacromolecules\| PubMed ID: 20942485	2010
Differences in cytotoxicity of Î²-sheet peptides originated from silk and amyloid Î². Macromolecular bioscience\| PubMed ID: 20954203	2011
Quantifying osteogenic cell degradation of silk biomaterials. Biomacromolecules\| PubMed ID: 21105641	2010
AFM study of morphology and mechanical properties of a chimeric spider silk and bone sialoprotein protein for bone regeneration. Biomacromolecules\| PubMed ID: 21370930	2011
State of water, molecular structure, and cytotoxicity of silk hydrogels. Biomacromolecules\| PubMed ID: 21517113	2011
Spider silk-based gene carriers for tumor cell-specific delivery. Bioconjugate chemistry\| PubMed ID: 21739966	2011
Silk-based nanocomplexes with tumor-homing peptides for tumor-specific gene delivery. Macromolecular bioscience\| PubMed ID: 22052706	2012
Biosynthesis of polyhydroxyalkanaotes by a novel facultatively anaerobic Vibrio sp. under marine conditions. Marine biotechnology (New York, N.Y.)\| PubMed ID: 22068389	2012
Chemoenzymatic synthesis of poly(L-alanine) in aqueous environment. Biomacromolecules\| PubMed ID: 22380731	2012
Biocompatible and biodegradable dual-drug release system based on silk hydrogel containing silk nanoparticles. Biomacromolecules\| PubMed ID: 22471816	2012
Active intermediates of polyhydroxyalkanoate synthase from Aeromonas caviae in polymerization reaction. Biomacromolecules\| PubMed ID: 23043466	2012
Rapid and efficient gene delivery into plant cells using designed peptide carriers. Biomacromolecules\| PubMed ID: 23215041	2013
Biopolymer-based nanoparticles for drug/gene delivery and tissue engineering. International journal of molecular sciences\| PubMed ID: 23344060	2013
Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]. AMB Express\| PubMed ID: 23351303	2013
Characterization of site-specific mutations in a short-chain-length/medium-chain-length polyhydroxyalkanoate synthase: in vivo and in vitro studies of enzymatic activity and substrate specif... Applied and environmental microbiology\| PubMed ID: 23584780	2013
Increased bioplastic production with an RNA polymerase sigma factor SigE during nitrogen starvation in Synechocystis sp. PCC 6803. DNA research : an international journal for rapid publication of reports on genes and genomes\| PubMed ID: 23861321	2013
Modification of β-oxidation pathway in Ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from soybean oil. Journal of bioscience and bioengineering\| PubMed ID: 23999062	2014
Proteinase K-catalyzed synthesis of linear and star oligo(L-phenylalanine) conjugates. Biomacromolecules\| PubMed ID: 24000943	2013
Short one-pot chemo-enzymatic synthesis of L-lysine and L-alanine diblock co-oligopeptides. Biomacromolecules\| PubMed ID: 24484289	2014
Pathway-level acceleration of glycogen catabolism by a response regulator in the cyanobacterium Synechocystis species PCC 6803. Plant physiology\| PubMed ID: 24521880	2014
Phasin proteins activate Aeromonas caviae polyhydroxyalkanoate (PHA) synthase but not Ralstonia eutropha PHA synthase. Applied and environmental microbiology\| PubMed ID: 24584238	2014
Silk-pectin hydrogel with superior mechanical properties, biodegradability, and biocompatibility. Macromolecular bioscience\| PubMed ID: 24610718	2014
Local gene silencing in plants via synthetic dsRNA and carrier peptide. Plant biotechnology journal\| PubMed ID: 24905384	2014
Synthesis of adhesive peptides similar to those found in blue mussel (Mytilus edulis) using papain and tyrosinase. Biomacromolecules\| PubMed ID: 25030620	2014
Synthesis of homopolypeptides by aminolysis mediated by proteases encapsulated in silica nanospheres. Macromolecular bioscience\| PubMed ID: 25154484	2014
Recent advances in chemoenzymatic peptide syntheses. Molecules (Basel, Switzerland)\| PubMed ID: 25191871	2014
Optimization of poly(N-isopropylacrylamide) as an artificial amidase. Biomacromolecules\| PubMed ID: 25488109	2015
Gene introduction into the mitochondria of Arabidopsis thaliana via peptide-based carriers. Scientific reports\| PubMed ID: 25583214	2015
Co-expression of two polyhydroxyalkanoate synthase subunits from Synechocystis sp. PCC 6803 by cell-free synthesis and their specific activity for polymerization of 3-hydroxybutyryl-coenzyme... Biochemistry\| PubMed ID: 25629766	2015
Chemo-Enzymatic Synthesis of Linear and Branched Cationic Peptides: Evaluation as Gene Carriers. Macromolecular bioscience\| PubMed ID: 25828913	2015
Use of extension-deformation-based crystallisation of silk fibres to differentiate their functions in nature. Soft matter\| PubMed ID: 26166211	2015
Chemoenzymatic Synthesis of Oligo(L-cysteine) for Use as a Thermostable Bio-Based Material. Macromolecular bioscience\| PubMed ID: 26388290	2016
The Benzyl Ester Group of Amino Acid Monomers Enhances Substrate Affinity and Broadens the Substrate Specificity of the Enzyme Catalyst in Chemoenzymatic Copolymerization. Biomacromolecules\| PubMed ID: 26620763	2016
Influence of Water Content on the β-Sheet Formation, Thermal Stability, Water Removal, and Mechanical Properties of Silk Materials. Biomacromolecules\| PubMed ID: 26835719	2016
Papain-Catalyzed Chemoenzymatic Synthesis of Telechelic Polypeptides Using Bis(Leucine Ethyl Ester) Initiator. Macromolecular bioscience\| PubMed ID: 26947148	2016
Biosynthesis of polyhydroxyalkanoates containing hydroxyl group from glycolate in Escherichia coli. AMB Express\| PubMed ID: 27075993	2016
Oil-free hyaluronic acid matrix for serial femtosecond crystallography. Scientific reports\| PubMed ID: 27087008	2016
Intracellular Delivery of Proteins via Fusion Peptides in Intact Plants. PloS one\| PubMed ID: 27100681	2016
Relationships between physical properties and sequence in silkworm silks. Scientific reports\| PubMed ID: 27279149	2016
Synthesis of High-Molecular-Weight Polyhydroxyalkanoates by Marine Photosynthetic Purple Bacteria. PloS one\| PubMed ID: 27513570	2016
Enzyme-Mimic Peptide Assembly To Achieve Amidolytic Activity. Biomacromolecules\| PubMed ID: 27642764	2016
Self-Assembled Peptide-Based System for Mitochondrial-Targeted Gene Delivery: Functional and Structural Insights. Biomacromolecules\| PubMed ID: 27696822	2016
A Screening Method for the Isolation of Polyhydroxyalkanoate-Producing Purple Non-sulfur Photosynthetic Bacteria from Natural Seawater. Frontiers in microbiology\| PubMed ID: 27708640	2016
Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin... AMB Express\| PubMed ID: 27730572	2016