ligand-nano-cluster-arrays-in-a-supported-lipid-bilayer

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Currently there is considerable interest in creating ordered arrays of adhesive protein islands in a sea of passivated surface for cell biological ...

CNRS, UMR 7325, CINaM

Field effect transistors have risen as one of the most promising techniques in the development of biomedical diagnosis and monitoring. In such devices, the sensitivity and specificity of the sensor rely on the properties of the active sensing layer (gate dielectric and probe layer). We propose here a new type of transistor developed for the detection of Fe(3+) ions in which this sensing layer is made of a monolayer of lipids, engineered in such a way that it is not sensitive to pH in the acidic range, therefore making the device perfectly suitable for biomedical diagnosis. Probes are γ-pyrone derivatives that have been grafted to the lipid headgroups. Affinity constants derived for the chelator/Fe(3+) complexation as well as for other ions demonstrate very high sensitivity and specificity towards ferric ions with values as high as 5.10(10) M and a detected concentration as low as 50 fM.

A field effect transistor biosensor with a γ-pyrone derivative engineered lipid-sensing layer for ultrasensitive Fe3+ ion detection with low pH interference.

Arrays of protein nanodots with dot-size tuned independently of spacing (e.g., ∼100 to 600 nm diameter for 900 nm spacing) are fabricated. The mechanism of size control is demonstrated, by numerical simulations, to arise from shadow effects during deposition of a sacrificial metal mask. We functionalize the nanodots with antibodies and embed them in a polymer-cushion or in lipid-bilayers or transfer them to soft elastomers. Their ability to influence cell architecture and local membrane organization is demonstrated in T-lymphocytes, using reflection interference contrast and total internal reflection fluorescence microscopy.

Size-Tunable Organic Nanodot Arrays: A Versatile Platform for Manipulating and Imaging Cells.

We demonstrate in this work that the indirect nanoplasmonic sensing lets us follow the adsorption/desorption of water molecules on soot particles that are a major contributor of the global warming. Increasing the relative humidity of the surrounding medium we measure a shift in wavelength of the localized surface plasmon resonance response of gold nanodisks on which soot particles are deposited. We show a singular and reversible blue shift with hydrophilic aircraft soot particles interpreted from a basic model as a reversible morphological change of the soot aggregates. This new method is highly sensitive and interesting to follow the change of optical properties of aerosols during their aging in the atmosphere, where they can adsorb and react with different gas molecules.

Indirect Nanoplasmonic Sensing to Probe with a High Sensitivity the Interaction of Water Vapor with Soot Aerosols.

Light reflection occuring at the surface of silicon wafers is drastically diminished by etching square pillars of height 110 nm and width 140 nm separated by a 100 nm gap distance in a square lattice. The design of the nanostructure is optimized to widen the spectral tolerance of the antireflective coatings over the visible spectrum for both fundamental polarizations. Angle and polarized resolved optical measurements report a light reflection remaining under 5% when averaged in the visible spectrum for both polarizations in a wide angular range. Light reflection remains almost insensitive to the light polarization even in oblique incidence.

Optimized 2D array of thin silicon pillars for efficient antireflective coatings in the visible spectrum.

Plasmonic antennas have a profound impact on nanophotonics as they provide efficient means to manipulate light and enhance light-matter interactions at the nanoscale. However, the large absorption losses found in metals can severely limit the plasmonic applications in the visible spectral range. Here, we demonstrate the effectiveness of an alternative approach using all-dielectric nanoantennas based on silicon dimers to enhance the fluorescence detection of single molecules. The silicon antenna design is optimized to confine the near field intensity in the 20~nm nanogap and reach a 270-fold fluorescence enhancement in a nanoscale volume of $\lambda^3/1800$ with dielectric materials only. Our conclusions are assessed by combining polarization resolved optical spectroscopy of individual antennas, scanning electron microscopy, numerical simulations, fluorescence lifetime measurements, burst analysis and fluorescence correlation spectroscopy. This work demonstrates that all-silicon nanoantennas are a valid alternative to plasmonic devices for enhanced single molecule fluorescence sensing, with the additional key advantages of reduced nonradiative quenching, negligible heat generation, cost-efficiency and CMOS compatibility.

All-Dielectric Silicon Nanogap Antennas to Enhance the Fluorescence of Single Molecules.

The photonic resonances hosted by nanostructures provide vivid colors that can be used as color filters instead of organic colors and pigments in photodetectors and printing technology. Metallic nanostructures have been widely studied due to their ability to sustain surface plasmons that resonantly interact with light. Most of the metallic nanoparticles behave as point-like electric multipoles. However, the needs of an another degree of freedom to tune the color of the photonic nanostructure together with the use of a reliable and cost-effective material are growing. Here, we report a technique to imprint colored images based on silicon nanoparticles that host low-order electric and magnetic Mie resonances. The interplay between the electric and magnetic resonances leads to a large palette of colors. This all-dielectric fabrication technique offers the advantage to use cost-effective, reliable, and sustainable materials to provide vivid color spanning the whole visible spectrum. The interest and potential of this all-dielectric printing technique are highlighted by reproducing at a micrometer scale a Mondrian painting.

Article	Year
A field effect transistor biosensor with a γ-pyrone derivative engineered lipid-sensing layer for ultrasensitive Fe3+ ion detection with low pH interference. Biosensors & bioelectronics\| PubMed ID: 24333568	2014
Size-Tunable Organic Nanodot Arrays: A Versatile Platform for Manipulating and Imaging Cells. Nano letters\| PubMed ID: 26161675	2015
Indirect Nanoplasmonic Sensing to Probe with a High Sensitivity the Interaction of Water Vapor with Soot Aerosols. The journal of physical chemistry letters\| PubMed ID: 26722790	2015
Optimized 2D array of thin silicon pillars for efficient antireflective coatings in the visible spectrum. Scientific reports\| PubMed ID: 27109643	2016
All-Dielectric Silicon Nanogap Antennas to Enhance the Fluorescence of Single Molecules. Nano letters\| PubMed ID: 27399057	2016
All-Dielectric Colored Metasurfaces with Silicon Mie Resonators. ACS nano\| PubMed ID: 27458790	2016

Igor Ozerov

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