chemical-analysis-water-accommodated-fractions-crude-oil-spills-using

Chemical Analysis of Water-accommodated Fractions of Crude Oil Spills Using TIMS-FT-ICR MS

Multiple chemical processes control how crude oil is incorporated into seawater and also the chemical reactions that occur overtime. Studying this system ...

Florida International University

With the advent of high resolution ion mobility analyzers and their coupling to ultrahigh resolution mass spectrometers, there is a need to further develop a theoretical workflow capable of correlating experimental accurate mass and mobility measurements with tridimensional candidate structures. In the present work, a general workflow is described for unsupervised tridimensional structural assignment based on accurate mass measurements, mobility measurements, in silico 2D-3D structure generation, and theoretical mobility calculations. In particular, the potential of this workflow will be shown for the analysis of polyaromatic hydrocarbons from Coal Tar SRM 1597a using selected accumulation - trapped ion mobility spectrometry (SA-TIMS) coupled to Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS). The proposed workflow can be adapted to different IMS scenarios, can utilize different collisional cross-section calculators and has the potential to include MS(n) and IMS(n) measurements for faster and more accurate tridimensional structural assignment.

Towards unsupervised polyaromatic hydrocarbons structural assignment from SA-TIMS-FTMS data.

In the present work, we demonstrate the potential and versatility of TIMS for the analysis of proteins, DNA-protein complexes and protein-protein complexes in their native and denatured states. In addition, we show that accurate CCS measurement are possible and in good agreement with previously reported CCS values using other IMS analyzers (<5% difference). The main challenges for the analysis of high mass proteins and protein complexes in the mobility and m/z domain are described. That is, the analysis of high molecular weight systems in their native state may require the use of higher electric fields or a compromise in the TIMS mobility resolution by reducing the bath gas velocity in order to effectively trap at lower electric fields. This is the first report of CCS measurements of high molecular weight biomolecules and biomolecular complexes (~ 150 kDa) using TIMS-MS.

Article	Year
Towards unsupervised polyaromatic hydrocarbons structural assignment from SA-TIMS-FTMS data. International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry\| PubMed ID: 26525904	2015
Towards the Analysis of High Molecular Weight Proteins and Protein complexes using TIMS-MS. International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry\| PubMed ID: 27818614	2016

Rebecca Marin

Biography

JoVE Journal Publications

Other Publications