a-novel-method-for-situ-electromechanical-characterization-nanoscale

A Novel Method for <em>In Situ</em> Electromechanical Characterization of Nanoscale Specimens

Electrically assisted deformation (EAD) is increasingly being used to improve the formability of metals during processes such as sheet metal rolling and ...

US Naval Research Laboratory

American Society for Engineering Education—Naval Research Laboratory (ASEE-NRL)

A contact lens biofuel cell was fabricated using buckypaper electrodes cured on a silicone elastomer soft contact lens. The buckypaper anode consisted of poly(methylene green) and a hydrogel matrix containing lactate dehydrogenase and nicotinamide adenine dinucleotide hydrate (NAD(+)). The buckypaper cathode was modified with 1-pyrenemethyl anthracene-2-carboxylate, and then bilirubin oxidase was immobilized within a polymer. Contact lens biofuel cell testing was performed in a synthetic tear solution at 35°C. The open circuit voltage was 0.413±0.06 V and the maximum current and power density were 61.3±2.9 µA cm(-2) and 8.01±1.4 µWc m(-2), respectively. Continuous operation for 17h revealed anode instability as output current rapidly decreased in the first 4h and then stabilized for the next 13 h. The contact lens biofuel cell presented here is a step toward achieving self-powered electronic contact lenses and ocular devices with an integrated power source.

Contact lens biofuel cell tested in a synthetic tear solution.

Lactate is an important biomarker due to its excessive production by the body during anerobic metabolism. Existing methods for electrochemical lactate detection require the use of an external power source to supply a positive potential to the working electrode of a given device. Herein we describe a self-powered amperometric lactate biosensor that utilizes a dimethylferrocene-modified linear poly(ethylenimine) (FcMe2-LPEI) hydrogel to simultaneously immobilize and mediate electron transfer from lactate oxidase (LOx) at the anode and a previously described enzymatic cathode. Operating as a half-cell, the FcMe2-LPEI electrode material generates a jmax of 1.51 ± 0.13 mAcm(-2) with a KM of 1.6 ± 0.1 mM and a sensitivity of 400 ± 20 μAcm(-2)mM(-1) while operating with an applied potential of 0.3 V vs. SCE. When coupled with an enzymatic biocathode, the self-powered biosensor has a detection range between 0mM and 5mM lactate with a sensitivity of 45 ± 6 μAcm(-2)mM(-1). Additionally, the FcMe2-LPEI/LOx-based self-powered sensor is capable of generating a power density of 122 ± 5 μWcm(-2) with a current density of 657 ± 17 μAcm(-2) and an open circuit potential of 0.57 ± 0.01 V, which is sufficient to act as a supplemental power source for additional small electronic devices.

Article	Year
Contact lens biofuel cell tested in a synthetic tear solution. Biosensors & bioelectronics\| PubMed ID: 25562741	2015
A self-powered amperometric lactate biosensor based on lactate oxidase immobilized in dimethylferrocene-modified LPEI. Biosensors & bioelectronics\| PubMed ID: 26385734	2016

Russell C. Reid

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