determination-sialic-acids-liver-milk-samples-wild-type-cmah-knock

Determination of Sialic Acids in Liver and Milk Samples of Wild-type and <em>CMAH</em> Knock-out Mice.

CMAH (cytidine monophosphate-N-acetylneuraminic acid hydroxylase) is responsible for the oxidation of cytidine monophosphate-N-acetylneuraminic acids in ...

Nanjing Agricultural University

While many studies of cis-elements CArG bound by serum response factor (SRF) are in progress, little is known about the positional distribution of the functional CArG elements around the transcription start site (TSS) of genes that they influence. We use a validated CArG data set to calculate the distance distribution of functional CArG elements around the TSS. Distances between adjacent CArGs were also analyzed. We compare these distributions with those derived using a control set of randomly selected CArGs (that were not experimentally validated for function). Our results show that most functional CArG elements (108 of 152, 71%) exist upstream of the annotated TSS, with copy number increasing as one moves closer to the TSS. Moreover, the average number of the CArG elements in the CArG-containing genes is significantly more than that in the control genes. Our study extends earlier bioinformatic analyses of functional CArG elements and provides an application of comparative sequence data to the identification of transcription factor binding sites.

The correlations of the function and positional distribution of the cis-elements CArG around the TSS in the genes of Mus musculus.

Currently, morphological criteria are used to select embryos for transfer in in vitro fertilization (IVF)-embryo transfer. However, the implantation rate is only about 30%, indicating a need for a more efficient method of selecting high-quality embryos. This study investigated the relationship between medium ammonium concentration and IVF implantation rates and evaluated the possibility of using ammonium concentration to provide an objective, noninvasive, and simple means of assessment of embryo viability.

Ammonium concentration of spent medium provides a noninvasive assessment of embryonic developmental potential in IVF.

Oak decline is a process induced by complex interactions of predisposing factors, inciting factors, and contributing factors operating at tree, stand, and landscape scales. It has greatly altered species composition and stand structure in affected areas. Thinning, clearcutting, and group selection are widely adopted harvest alternatives for reducing forest vulnerability to oak decline by removing susceptible species and declining trees. However, the long-term, landscape-scale effects of these different harvest alternatives are not well studied because of the limited availability of experimental data. In this study, we applied a forest landscape model in combination with field studies to evaluate the effects of the three harvest alternatives on mitigating oak decline in a Central Hardwood Forest landscape. Results showed that the potential oak decline in high risk sites decreased strongly in the next five decades irrespective of harvest alternatives. This is because oak decline is a natural process and forest succession (e.g., high tree mortality resulting from intense competition) would eventually lead to the decrease in oak decline in this area. However, forest harvesting did play a role in mitigating oak decline and the effectiveness varied among the three harvest alternatives. The group selection and clearcutting alternatives were most effective in mitigating oak decline in the short and medium terms, respectively. The long-term effects of the three harvest alternatives on mitigating oak decline became less discernible as the role of succession increased. The thinning alternative had the highest biomass retention over time, followed by the group selection and clearcutting alternatives. The group selection alternative that balanced treatment effects and retaining biomass was the most viable alternative for managing oak decline. Insights from this study may be useful in developing effective and informed forest harvesting plans for managing oak decline.

Modeling the Effects of Harvest Alternatives on Mitigating Oak Decline in a Central Hardwood Forest Landscape.

The prognostic value of human epidermal growth factor receptor 2 (HER2) in esophageal carcinoma (EC) remains controversial. Our study aims to clarify the correlation between HER2 overexpression and survival in patients with EC.

Role of human epidermal growth factor receptor 2 as a prognostic factor for survival in esophageal carcinoma: evidence from 2872 subjects.

INTRODUCTIONː The prognostic value of c-Met in patients with esophageal cancer (EC) remains inconsistent and controversial. Our study aims to clarify the correlation between c-Met overexpression and clinical outcome in EC patients. EVIDENCE ACQUISITIONː We performed a comprehensive search of EMBASE, PubMed, Web of Science, Chinese National Knowledge Infrastructure (CNKI) and Chinese Biomedical Database (CBM) (from inception to May 1, 2016) for published literature regarding the potential association between c-Met overexpression and clinical outcome in EC patients. A fixed-effects or random-effects model according to heterogeneity was applied to calculate the pooled hazard ratios (HRs) with 95% confidence intervals (CIs) for overall survival (OS), disease-free survival (DFS) and disease-specific survival (DSS). EVIDENCE SYNTHESISː Nine eligible studies totaling 1062 patients were identified in this meta-analysis. C-Met overexpression was significantly associated with shorter OS (HR: 2.04, 95% CI: 1.66-2.52, P<0.001) and DSS (HR: 3.03, 95% CI: 2.04-4.48, P<0.001) in patients with EC. However, no significant relationship between high expression of c-Met and DFS that was found (HR: 1.81, 95% CI: 0.77-4.26, P=0.176). For OS, similar associations were demonstrated in either esophageal squamous cell carcinoma (ESCC) (HR: 2.17, 95% CI: 1.62-2.90, P<0.001) or esophageal adenocarcinoma (EAC) (HR: 1.92, 95% CI: 1.42-2.59, P<0.001). Additionally, further subgroup analyses according to publication year, ethnicity, the sample size, and statistical methodology all revealed a significant association between high expression of c-Met and OS in patients with EC. CONCLUSIONSː The current evidence indicated that c-Met overexpression is significantly associated with a poorer prognosis in EC. C-Met may serve as a potential novel prognostic biomarker for EC patients.

C-Met as a potential novel prognostic marker in squamous cell carcinoma and adenocarcinoma of esophagus: evidence from a meta-analysis.

The Central Hardwood Forest (CHF) in the United States is currently a major carbon sink, there are uncertainties in how long the current carbon sink will persist and if the CHF will eventually become a carbon source. We used a multi-model ensemble to investigate aboveground carbon density of the CHF from 2010 to 2300 under current climate. Simulations were done using one representative model for each of the simple, intermediate, and complex demographic approaches (ED2, LANDIS PRO, and LINKAGES, respectively). All approaches agreed that the current carbon sink would persist at least to 2100. However, carbon dynamics after current carbon sink diminishes to zero differ for different demographic modelling approaches. Both the simple and the complex demographic approaches predicted prolonged periods of relatively stable carbon densities after 2100, with minor declines, until the end of simulations in 2300. In contrast, the intermediate demographic approach predicted the CHF would become a carbon source between 2110 and 2260, followed by another carbon sink period. The disagreement between these patterns can be partly explained by differences in the capacity of models to simulate gross growth (both birth and subsequent growth) and mortality of short-lived, relatively shade-intolerant tree species.

Article	Year
The correlations of the function and positional distribution of the cis-elements CArG around the TSS in the genes of Mus musculus. Genome\| PubMed ID: 19234549	2009
Ammonium concentration of spent medium provides a noninvasive assessment of embryonic developmental potential in IVF. Reproductive sciences (Thousand Oaks, Calif.)\| PubMed ID: 23585343	2013
Modeling the Effects of Harvest Alternatives on Mitigating Oak Decline in a Central Hardwood Forest Landscape. PloS one\| PubMed ID: 23825560	2013
Role of human epidermal growth factor receptor 2 as a prognostic factor for survival in esophageal carcinoma: evidence from 2872 subjects. Minerva medica\| PubMed ID: 27309036	2016
C-Met as a potential novel prognostic marker in squamous cell carcinoma and adenocarcinoma of esophagus: evidence from a meta-analysis. Panminerva medica\| PubMed ID: 27636117	2017
Future forest aboveground carbon dynamics in the central United States: the importance of forest demographic processes. Scientific reports\| PubMed ID: 28165483	2017

Wen J. Wang

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