effectiveness-air-stripping-two-salmonid-fish-rainbow-trout

Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout (Oncorhynchus Mykiss) and Brown Trout (Salmo Trutta Morpha fario)

Egg collection is one of the most crucial procedures during fish reproduction in salmonid hatcheries. Classic methods involve the use of hand massage on ...

Polish academy of science

Multiple collections of semen during the reproductive period of the common carp Cyprinus carpio L. were used to analyse changes in semen quality. Semen collection was performed on June 1 (first collection), 12 (second collection), and 19 (third collection) from individual males (n=11) by gentle abdominal massage. Semen quantity (semen volume and sperm count), quality (sperm motility and sperm viability), as well as seminal plasma parameters (pH of seminal plasma and seminal plasma osmotic pressure) and its enzymatic activity, e.g., lactate dehydrogenase (LDH) and ß-N-acetylglucosaminidase (ß-NAG) were determined. Moreover, for the first time, the percentage of live, dead, and apoptotic sperm, as well as the proteolytic activity of seminal plasma, were determined using flow cytometry and zymography, respectively, at specific times during the common carp reproductive period. The lowest volumes of semen and sperm concentration were noted during the first semen collection (June 1). Analysis of computer-assisted sperm analysis parameters revealed the greatest sperm motility, sperm velocity, as well as amplitude of lateral head displacement, were evident in the third collection (June 19). There were no differences in progressively motile sperm, movement linearity, wobbling index, and beat cross frequency between the different collection times. The lowest percentage of live sperm was found in the first collection, although with the passage of time values of this parameter increased. Seminal plasma pH and seminal plasma osmotic pressure were at the lowest values in the second collection (June 12), which corresponded with the lowest concentration of sperm. In the first collection, seminal plasma contained the highest values of LDH and ß-NAG activity, whereas there were no differences in the proteolytic activity of seminal plasma determined between the different collections of semen. The results presented here indicate that during the reproductive period, males of common carp produce a large amount of semen of moderate quality. Low sperm motility noted in the second collection might be explained by a significant increase in sperm production during this period, followed by a low seminal plasma pH and high hydration rate. The high LDH and ß-NAG activity noted in the first collection of semen may reflect a reduced stability of the sperm cell membrane and its viability. The significant difference in the percentage of live sperm at June 1 compared to that at June 19 supports this hypothesis.

Multiple collections of common carp Cyprinus carpio L. semen during the reproductive period and its effects on sperm quality.

This study investigated how the inhibition of certain enzymes present in ide sperm influences sperm motility and the percentage of fertilised eggs. The enzymes studied were acid phosphatase (AcP), β-N-acetylglucosaminidase (β-NAGase) and lactate dehydrogenase (LDH). None of the inhibitors affected ide sperm motility parameters. The addition of gossypol (a LDH inhibitor) caused a considerable increase in the percentage of fertilised eggs (92-95% compared to 63% in the control). The inhibition of AcP caused a considerable decrease in fertility rate - at the highest inhibitor dose, the percentage of fertilised eggs decreased to 26%. A similar effect was seen after the addition of acetamide (a β-NAGase inhibitor), but in this case the highest dose caused complete inhibition of fertilisation. The results presented here indicate the importance of AcP and β-NAGase in the process of ide fertilisation.

The influence of inhibition of acid phosphatase, β-N-acetylglucosaminidase and lactate dehydrogenase present in the sperm of ide (Leuciscus idus) on the percentage of fertilised eggs.

Article	Year
Multiple collections of common carp Cyprinus carpio L. semen during the reproductive period and its effects on sperm quality. Animal reproduction science\| PubMed ID: 29223503	2018
The influence of inhibition of acid phosphatase, β-N-acetylglucosaminidase and lactate dehydrogenase present in the sperm of ide (Leuciscus idus) on the percentage of fertilised eggs. Animal reproduction science\| PubMed ID: 29807827	2018

Beata Sarosiek

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