visually-based-characterization-incipient-particle-motion-regular

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

Two different experimental methods for determining the threshold of particle motion as a function of geometrical properties of the bed from laminar to ...

Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)

Measuring rheological properties of cells in monolayers enables quantifying average cell properties in single experimental runs despite large cell-to-cell variations. Here, we describe how to modify a commercial rotational rheometer to accomplish the necessary precision for a monolayer rheometer and we delineate the steps for setting up experiments detecting average viscoelastic cell properties.

Average Rheological Quantities of Cells in Monolayers.

We study the adhesion limit of 3T6 fibroblasts, cultured in Dulbecco's modified Eagle's medium with 10% fetal bovine serum at 37 °C and 5% CO, with a narrow-gap rotational rheometer in the parallel-disk configuration. Reducing the uncertainty in gap width to about 1 µm allows studying the cells at narrow gaps, which enables to study the critical shear stress of the cells in low-viscous media. The adhesion limit on fibronectin-coated glass plates is determined as a function of concentration and adhesion time. We found that cells in groups have a tendency to detach at slightly higher shear stresses than single cells. Moreover, 60 min after the settling phase are enough for the cells to adhere to the coated plate at maximum strength. We show that the setup may also be used for cells that are not adhered from suspension, but are grown directly on the substrate.

Article	Year
Average Rheological Quantities of Cells in Monolayers. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 28470532	2017
Measuring the adhesion limit of fibronectin for fibroblasts with a narrow-gap rotational rheometer. Bioprocess and biosystems engineering\| PubMed ID: 29177680	2018

Andreas Wierschem

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