assessment-plasma-coagulation-on-liver-tissue-large-animal-model

Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model <em>In Vivo</em>

Plasma coagulation as a form of electrocautery is used in liver surgery for decades to seal the large liver cut surface after major hepatectomy to prevent ...

RWTH Aachen University

Energy-based technologies for tissue sealing and cutting are increasingly supplementing current standards used for haemostasis and dissection during laparoscopic surgery. For their safe and efficacious use in clinical practice, these instruments have to guarantee sufficient burst resistance and low thermal damage to adjacent tissue in combination with good cutting characteristics.

Evaluation of the novel bipolar vessel sealing and cutting device BiCision® in a porcine model.

The isolated perfused porcine kidney (IPPK) model has been the method of choice for the early preclinical evaluation of kidney graft preservation techniques. The preferred reperfusion conditions have not yet been determined. Here, we examined the effects of pressure- or flow-controlled perfusion and oxygenation by pure oxygen or carbogen (95% O2/5% CO2) on normothermic reperfusion in the IPPK model.

Determination of the preferred conditions for the isolated perfusion of porcine kidneys.

Ecosol, an extracellular-type, colloid-based preservation solution, has recently been introduced for washout, cold storage, and machine perfusion preservation of kidney grafts. Here, we assessed the efficacy of Ecosol compared to the widely used Histidine-Tryptophan-Ketoglutarate solution (HTK) for 24-h cold storage preservation of warm ischemia-damaged kidney grafts.

Improved preservation of warm ischemia-damaged porcine kidneys after cold storage in Ecosol, a novel preservation solution.

The global shortage of donor organs for transplantation has necessitated the expansion of the organ pool through increased use of organs from less ideal donors. Venous systemic oxygen persufflation (VSOP) and oxygenated machine perfusion (OMP) have previously demonstrated beneficial results compared to cold storage (CS) in the preservation of warm-ischemia-damaged kidney grafts. The aim of this study was to compare the efficacy of VSOP and OMP for the preservation of warm-ischemia-damaged porcine kidneys using the recently introduced Ecosol preservation solution compared to CS using Ecosol or histidine-tryptophan-ketoglutarate solution (HTK).

Comparison of Aerobic Preservation by Venous Systemic Oxygen Persufflation or Oxygenated Machine Perfusion of Warm-Ischemia-Damaged Porcine Kidneys.

The long-term outcome of intestinal transplantations is still not favorable, which is partly due to the intestinal susceptibility to ischemia. There are several indications that the inflammatory response to ischemia-reperfusion injury is mediated by cyclooxygenases and that their inhibition may be associated with improved organ function. The aim of this study was to analyze if cyclooxygenase (COX) inhibitors could improve the early posttransplant outcome after orthotopic small bowel transplantation.

Cyclooxygenase Inhibitors as a New Therapeutic Strategy in Small Bowel Transplantation.

Objective Argon plasma coagulation (APC) and helium plasma coagulation (HPC) are electrosurgical techniques that provide noncontact monopolar electrothermal haemostasis. Although these techniques have been widely used clinically during the last three decades, their in vivo effects on liver tissue remain unclear. Methods We investigated the effects of different power levels (10-100 W) of APC and HPC on liver coagulation in 11 Landrace pigs. Capillary blood flow and capillary blood flow velocity were recorded with a combined laser Doppler flowmeter and spectrophotometer. The temperature, clinical biochemical parameters, blood gas parameters, bile duct-sealing effect, and coagulation depth were measured. Results APC and HPC significantly reduced the capillary blood flow and capillary blood flow velocity compared with baseline flow. No significant temperature change was measured on the liver surface immediately after coagulation. The clinical biochemical and blood gas parameters were not different before and after coagulation. The coagulation depth was positively correlated with the device power setting. Conclusions These results prove that APC and HPC provide sufficient superficial haemostasis. No significant systemic effects occurred following coagulation. The depth of the coagulation effect can be controlled through selection of the output power level.

Article	Year
Evaluation of the novel bipolar vessel sealing and cutting device BiCision® in a porcine model. Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy\| PubMed ID: 22455599	2012
Determination of the preferred conditions for the isolated perfusion of porcine kidneys. European surgical research. Europäische chirurgische Forschung. Recherches chirurgicales européennes\| PubMed ID: 25341590	2015
Improved preservation of warm ischemia-damaged porcine kidneys after cold storage in Ecosol, a novel preservation solution. Annals of transplantation\| PubMed ID: 25912926	2015
Comparison of Aerobic Preservation by Venous Systemic Oxygen Persufflation or Oxygenated Machine Perfusion of Warm-Ischemia-Damaged Porcine Kidneys. European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes\| PubMed ID: 27070724	2016
Cyclooxygenase Inhibitors as a New Therapeutic Strategy in Small Bowel Transplantation. Transplantation\| PubMed ID: 27479158	2016
Argon and helium plasma coagulation of porcine liver tissue. The Journal of international medical research\| PubMed ID: 28661266	2017

Pascal Paschenda

Biography

JoVE Journal Publications

Other Publications