severe-burn-injury-in-a-swine-model-for-clinical-dressing-assessment

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

Wound healing is a dynamic repair process and is the most complex biological process in human life. In response to burn injury, alterations in biological ...

National Defense Medical Center

National Defense Medical University

Stem cells derived from oral tissue represent a highly attractive alternative source for clinical bone regeneration because they can be collected by non-invasive or minimally invasive procedures. Herein, we describe the human dental stem cells (DSCs) deriving from buccal fat pads (BFP), dental pulp (DP) of impacted teeth, and periodontal ligaments (PDL) to obtain BFPSCs, DPSCs, and PDLSCs, respectively. Cells were purified with selected medium and expanded through passages in stem cell culture medium. Purified cells were characterized for stemness by their growth rate, immunostaining, and multilineage differentiation ability. They showed plastic adherence, expression of stemness-specific markers, and multilineage differentiation potential. Immunocytochemistry analysis confirmed that DPSCs had more osteogenic potential than BFSCs and PDLSCs. Calcium-rich deposits, evaluated by von Kossa and Alizarin red staining, showed greater mineralization when DPSCs were cultured on collagen type I matrix than without collagen. Furthermore, DPSC-seeded collagen type I matrix maintained consistent osteogenesis and boosted mineral formation by 1-2 weeks over that in DPSCs cultured without collagen. Radiographic analysis of DPSC-seeded collagen type I matrix transplanted into rat cranial defects showed significant bone regeneration after 8 weeks. These results suggested that the redundant oral tissue can be used as a source of adult multipotent stem cells for clinical bone regeneration. Triple overlay images with biomarkers (red), nuclei (blue) and bright field morphology of DPSCs. The specifically osteo-differentiation shown by osteocalcin (left) expression and lack of sox9 (right) expressed in the images below which were cultured with collagen matrix, contrast with no collagen matrix group above.

Osteogenic prospective of deriving human dental stem cells in collagen matrix boost.

The pathogenesis of ketamine cystitis (KC) has been recently linked with immune response to patients but the same has not yet been established. Hence, this study aims to propose a possible immune mechanism of irreversible bladder damage caused by KC. A total of 53 KC patients and 21 healthy volunteers as controls have been retrospectively assessed. The levels of serum immunoglobulin E (IgE), IL-6, and IFN- of KC patients were significantly higher than those of controls, whereas the TGF- levels of KC patients substantially reduced but the IL-2 and IL-4 levels of KC patients were comparable to those of controls. Moreover, the KC patients had significantly higher counts of T1, T2, and T17 cells than those of controls. The immune response of KC users may begin with the IL-6 production and differentiation of T17 and may be followed by alternating between high expressions of T1 and T2. The IL-6 may further suppress the T cells which can aggravate chronic inflammation in KC patients and the imbalance in T17 and T cells may involve the pathogenesis of KC. Further investigation is needed to define the role of IL-6 in T1/T2/T17-regulated signaling pathway in ketamine-induced cystitis.

The Immunomodulatory Imbalance in Patients with Ketamine Cystitis.

The original version of this article unfortunately contained a mistake. The country was incorrect in the authors affiliations. It should read as "ROC". The corrected affiliations are given below.

Article	Year
Osteogenic prospective of deriving human dental stem cells in collagen matrix boost. Journal of materials science. Materials in medicine\| PubMed ID: 29143185	2017
The Immunomodulatory Imbalance in Patients with Ketamine Cystitis. BioMed research international\| PubMed ID: 29204439	2017
Correction to: Osteogenic prospective of deriving human dental stem cells in collagen matrix boost. Journal of materials science. Materials in medicine\| PubMed ID: 29946882	2018

Raju Poongodi

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