echocardiographic-measurement-right-ventricular-diastolic-parameters

Echocardiographic Measurement of Right Ventricular Diastolic Parameters in Mouse

Diastolic dysfunction is a prominent feature of right ventricular (RV) remodeling associated with conditions of pressure overload. However, the RV ...

Ludwig Boltzmann Institute for Lung Vascular Research

Acute respiratory distress syndrome (ARDS) is a major cause of mortality in intensive care units. As there is rising evidence about immuno-modulatory effects of lipid emulsions required for parenteral nutrition of ARDS patients, we sought to investigate whether infusion of conventional soybean oil (SO)-based or fish oil (FO)-based lipid emulsions rich in either n-6 or n-3 fatty acids, respectively, may influence subsequent pulmonary inflammation.

Immunomodulation by lipid emulsions in pulmonary inflammation: a randomized controlled trial.

Increasing evidence points towards an inflammatory component underlying pulmonary hypertension. However, the conclusive characterisation of multiple inflammatory cell populations in the lung is challenging due to the complexity of marker specificity and tissue inaccessibility. We used an unbiased computational flow cytometry approach to delineate the inflammatory landscape of idiopathic pulmonary arterial hypertension (IPAH) and healthy donor lungs.Donor and IPAH samples were discriminated clearly using principal component analysis to reduce the multidimensional data obtained from single-cell flow cytometry analysis. In IPAH lungs, the predominant CD45 cell type switched from neutrophils to CD3 T-cells, with increases in CD4, CD8 and γδT-cell subsets. Additionally, diversely activated classical myeloid-derived dendritic cells (CD14HLA-DRCD11cCD1a) and nonclassical plasmacytoid dendritic cells (pDCs; CD14CD11cCD123HLA-DR), together with mast cells and basophils, were more abundant in IPAH samples. We describe, for the first time, the presence and regulation of two cell types in IPAH, γδT-cells and pDCs, which link innate and adaptive immunity.With our high-throughput flow cytometry with multidimensional dataset analysis, we have revealed the interactive interplay between multiple inflammatory cells is a crucial part of their integrative network. The identification of γδT-cells and pDCs in this disease potentially provides a missing link between IPAH, autoimmunity and inflammation.

The inflammatory cell landscape in the lungs of patients with idiopathic pulmonary arterial hypertension.

Remodeling and fibrosis of the right ventricle (RV) may cause RV dysfunction and poor survival in patients with pulmonary hypertension (PH).

Article	Year
Immunomodulation by lipid emulsions in pulmonary inflammation: a randomized controlled trial. Critical care (London, England)\| PubMed ID: 25962383	2015
The inflammatory cell landscape in the lungs of patients with idiopathic pulmonary arterial hypertension. The European respiratory journal\| PubMed ID: 29371380	2018
Disconnect between Fibrotic Response and Right Ventricular Dysfunction. American journal of respiratory and critical care medicine\| PubMed ID: 30557518	2018

Leigh M. Marsh

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