isolation-physiologically-active-thylakoids-their-use-energy

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Chloroplasts are the organelles in green plants responsible for carrying out numerous essential metabolic pathways, most notably photosynthesis. Within ...

University of California - Davis

Proteins in the chloroplast outer envelope membrane are nuclear encoded and post-translationally targeted to the chloroplast. The targeting and membrane insertion of these proteins is not well understood. Although early work suggested otherwise, the best-studied outer membrane proteins (OMPs) use both proteins within the chloroplast and NTPs for insertion. There have been conflicting reports in the field regarding protein targeting and insertion, which have probably arisen because of differences in experimental methodology and different interpretations of reduction (versus abolition) of integration. This review summarizes what is known to date about the mechanism of chloroplast OMP targeting.

Chloroplast outer membrane protein targeting and insertion.

Chloroplast protein import is generally believed to occur posttranslationally through the interaction of a precursor protein with the Toc and Tic transport apparatus in the plastid envelope membranes. The cleavable N-terminal transit peptide present on translocated proteins has been considered to be essential and sufficient for targeting. This idea was recently challenged when an analysis of the chloroplast proteome revealed many proteins without a predicted transit peptide. A recent study demonstrates the existence of a novel chloroplast targeting pathway, starting with protein entry into the endoplasmic reticulum and involving the Golgi apparatus.

Evidence for an ER to Golgi to chloroplast protein transport pathway.

Photosynthetic electron transport pumps protons into the thylakoid lumen, creating an electrochemical potential called the protonmotive force (PMF). The energy of the thylakoid PMF is utilized by such machinery as the chloroplast F(0)F(1)-ATPase as well as the chloroplast Tat (cpTat) pathway (a protein transporter) to do work. The bulk phase thylakoid PMF decays rapidly after the termination of actinic illumination, and it has been well established via potentiometric measurements that there is no detectable electrical or chemical potential in the thylakoid after a brief time in the dark. Yet, we report herein that cpTat transport can occur for long periods in the dark. We show that the thylakoid PMF is actually present long after actinic illumination of the thylakoids ceases and that this energy is present in physiologically useful quantities. Consistent with previous studies, the dark-persisting thylakoid potential is not detectable by established indicators. We propose that cpTat transport in the dark is dependent on a pool of protons in the thylakoid held out of equilibrium with those in the bulk aqueous phase.

The chloroplast Tat pathway transports substrates in the dark.

The protein complexes in the thylakoid membrane are composed of subunits derived from both the nuclear and chloroplast genomes. While less is known about the mechanisms of delivery of the plastid-encoded subunits, the targeting mechanisms of the nuclear-encoded subunits have been more experimentally tractable. We have described in this chapter the methods used in our laboratory for investigations of the import of nuclear-encoded proteins across the chloroplast envelope membranes, and for their further delivery into or across the thylakoid membrane by one of the four distinct pathways.

Protein targeting across and into chloroplast membranes.

Measurement of the different components of the proton motive force (pmf) gives information about the coupling of proton movement within thylakoids to chemiosmotic processes such as photophosphorylation and protein transport, as well as that relating to the overall quality of a thylakoid preparation. The techniques to assess the pmf have been known for many years, as they have been applied to the most popular model plants for photosynthetic research. The emergence of Arabidopsis thaliana as the prominent model plant in developmental and genetics research prompted us to apply these techniques to thylakoids isolated from Arabidopsis chloroplasts. We describe here two spectroscopic techniques to measure the transmembrane pH gradient and electric field developed in the light in Arabidopsis thylakoids.

Measurement of the ΔpH and electric field developed across Arabidopsis thylakoids in the light.

Chloroplasts are essential organelles in the cells of plants and algae. The functions of these specialized plastids are largely dependent on the ~3000 proteins residing in the organelle. Although chloroplasts are capable of a limited amount of semiautonomous protein synthesis - their genomes encode ~100 proteins - they must import more than 95% of their proteins after synthesis in the cytosol. Imported proteins generally possess an N-terminal extension termed a transit peptide. The importing translocons are made up of two complexes in the outer and inner envelope membranes, the so-called Toc and Tic machineries, respectively. The Toc complex contains two precursor receptors, Toc159 and Toc34, a protein channel, Toc75, and a peripheral component, Toc64/OEP64. The Tic complex consists of as many as eight components, namely Tic22, Tic110, Tic40, Tic20, Tic21 Tic62, Tic55 and Tic32. This general Toc/Tic import pathway, worked out largely in pea chloroplasts, appears to operate in chloroplasts in all green plants, albeit with significant modifications. Sub-complexes of the Toc and Tic machineries are proposed to exist to satisfy different substrate-, tissue-, cell- and developmental requirements. In this review, we summarize our understanding of the functions of Toc and Tic components, comparing these components of the import machinery in green algae through trees. We emphasize recent findings that point to growing complexities of chloroplast protein import process, and use the evolutionary relationships between proteins of different species in an attempt to define the essential core translocon components and those more likely to be responsible for regulation. This article is part of a Special Issue entitled: Protein Import and Quality Control in Mitochondria and Plastids.

The chloroplast protein import system: from algae to trees.

Glutamine synthetase (GS; EC 6.3.1.2) plays a crucial role in the assimilation and re-assimilation of ammonia derived from a wide variety of metabolic processes during plant growth and development. Here, three developmentally regulated isoforms of GS holoenzyme in the leaf of wheat (Triticum aestivum L.) seedlings are described using native-PAGE with a transferase activity assay. The isoforms showed different mobilities in gels, with GSII>GSIII>GSI. The cytosolic GSI was composed of three subunits, GS1, GSr1, and GSr2, with the same molecular weight (39.2kDa), but different pI values. GSI appeared at leaf emergence and was active throughout the leaf lifespan. GSII and GSIII, both located in the chloroplast, were each composed of a single 42.1kDa subunit with different pI values. GSII was active mainly in green leaves, while GSIII showed brief but higher activity in green leaves grown under field conditions. LC-MS/MS experiments revealed that GSII and GSIII have the same amino acid sequence, but GSII has more modification sites. With a modified blue native electrophoresis (BNE) technique and in-gel catalytic activity analysis, only two GS isoforms were observed: one cytosolic and one chloroplastic. Mass calibrations on BNE gels showed that the cytosolic GS1 holoenzyme was ~490kDa and likely a dodecamer, and the chloroplastic GS2 holoenzyme was ~240kDa and likely a hexamer. Our experimental data suggest that the activity of GS isoforms in wheat is regulated by subcellular localization, assembly, and modification to achieve their roles during plant development. 

New isoforms and assembly of glutamine synthetase in the leaf of wheat (Triticum aestivum L.).

Stromules are stroma-containing tubules that have been observed to emanate from the main plastidic body in vivo. These structures have been shown to require cytoskeletal components for movement. Though numerous studies have shown a close association with the endoplasmic reticulum, nucleus, mitochondria, and other plastids, the mechanism of formation and their overall function remain unknown. A limiting factor in studying these structures has been the lack of a reconstituted system for in vitro stromule formation. In this study, stromule formation was induced in vitro by adding a plant extract fraction that is greater than 100 kDa to a population of isolated chloroplasts. Kinetic measurements show that stromule formation occurs within ~10 seconds after the addition of the plant extract fraction. Heat inactivation and apyrase treatment reveal that the stromule stimulating compound found in the extract fraction is a protein or protein complex 100 kDa or greater. The formation of the stromules in vitro with isolated chloroplasts and a concentrated fraction of cell extract opens an avenue for the biochemical dissection of this process that has heretofore been studied only in vivo. 

The Formation of Stromules In Vitro from Chloroplasts Isolated from Nicotiana benthamiana.

The ΔpH-dependent/Tat pathway is unique for using only the proton motive force for driving proteins transport across the thylakoid membrane in chloroplasts. 9-aminoacridine fluorescence quenching is widely used to monitor the ΔpH developed across the thylakoid membrane in the light. However, this method suffers from limited sensitivity to low ΔpH values and to spurious fluorescence signals due to membrane binding. In order to develop a more sensitive method for monitoring the real pH of the thylakoid lumen without these problems we transformed Arabidopsis thaliana with a ratiometric pH-sensitive GFP variant (termed pHluorin) targeted to the lumen by the prOE17 transit peptide. Positive transgenic plants displayed localization of pHluorin in the chloroplast by confocal microscopy, and fractionation experiments revealed that it is in the lumen. The pHluorin signal was the strongest in very young plants and diminished as the plants matured. The pHluorin released from the lumen displayed the expected fluorescence intensity changes in response to pH titration. The fluorescence signal in isolated chloroplasts responded to illumination in a manner consistent with light-dependent lumen acidification. Future experiments will exploit the use of this new pH-indicating probe of the thylakoid lumen to examine the influence of the thylakoid ΔpH on ATP synthesis and protein transport.

A new fluorescence-based method to monitor the pH in the thylakoid lumen using GFP variants.

The degree of residual structure retained by proteins while passing through biological membranes is a fundamental mechanistic question of protein translocation. Proteins are generally thought to be unfolded while transported through canonical proteinaceous translocons, including the translocons of the outer and inner chloroplast envelope membranes (TOC and TIC). Here, we readdressed the issue and found that the TOC/TIC translocons accommodated the tightly folded dihydrofolate reductase (DHFR) protein in complex with its stabilizing ligand, methotrexate (MTX). We employed a fluorescein-conjugated methotrexate (FMTX), which has slow membrane transport rates relative to unconjugated MTX, to show that the rate of ligand accumulation inside chloroplasts is faster when bound to DHFR that is actively being imported. Stromal accumulation of FMTX is ATP-dependent when DHFR is actively being imported, but is otherwise ATP-independent, again indicating DHFR/FMTX complex import. Furthermore, the TOC/TIC pore size was probed with fixed-diameter particles and found to be greater than 25.6 Å, large enough to support folded DHFR import and also larger than mitochondrial and bacterial protein translocons that have a requirement for protein unfolding. This unique pore size and the ability to import folded proteins has critical implications regarding the mechanism and structure of the TOC/TIC translocons.

Article	Year
Chloroplast outer membrane protein targeting and insertion. Trends in plant science\| PubMed ID: 16085449	2005
Evidence for an ER to Golgi to chloroplast protein transport pathway. Trends in cell biology\| PubMed ID: 16815014	2006
The chloroplast Tat pathway transports substrates in the dark. The Journal of biological chemistry\| PubMed ID: 18187420	2008
Protein targeting across and into chloroplast membranes. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 20960128	2011
Measurement of the ΔpH and electric field developed across Arabidopsis thylakoids in the light. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 21863452	2011
The chloroplast protein import system: from algae to trees. Biochimica et biophysica acta\| PubMed ID: 23063942	2013
New isoforms and assembly of glutamine synthetase in the leaf of wheat (Triticum aestivum L.). Journal of experimental botany\| PubMed ID: 26307137	2015
The Formation of Stromules In Vitro from Chloroplasts Isolated from Nicotiana benthamiana. PloS one\| PubMed ID: 26840974	2016
A new fluorescence-based method to monitor the pH in the thylakoid lumen using GFP variants. Biochemical and biophysical research communications\| PubMed ID: 27940360	2017
Evaluating the Functional Pore Size of Chloroplast TOC and TIC Protein Translocons: Import of Folded Proteins. The Plant cell\| PubMed ID: 30104404	2018

Steven M. Theg

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