visualizing-tracking-endogenous-mrnas-live-drosophila-melanogaster

Visualizing and Tracking Endogenous mRNAs in Live <em>Drosophila melanogaster</em> Egg Chambers

Fluorescence-based imaging techniques, in combination with developments in light microscopy, have revolutionized how cell biologists conduct live cell ...

CUNY School of Medicine

Graduate Center, City University of New York

City University of New York

The aim of this study was to perform a return-on-investment (ROI) analysis of a breast cancer screening program in Egypt by comparing net profit in treatment costs saved to program cost investment.

Return on Investment Analysis of Breast Cancer Screening and Downstaging in Egypt: Implications for Developing Countries.

Molecular beacons are nucleic acid oligomers labeled with a fluorophore and a quencher that fold in a hairpin-shaped structure, which fluoresce only when bound to their target RNA. They are used for the visualization of endogenous mRNAs in live cells. Here, we report a Python program () that designs molecular beacons best suited for live cell imaging by using structural information from secondary structures of the target RNA, predicted via energy minimization approaches.  takes into account the accessibility of the targeted regions, as well as the inter- and intramolecular interactions of each selected probe. To demonstrate its applicability, we synthesized an  mRNA-specific molecular beacon (osk1236), which is selected by  to target a more accessible region than a manually designed -specific molecular beacon (osk2216). We previously demonstrated osk2216 to be efficient in detecting  mRNA in in vivo experiments. Here, we show that osk1236 outperformed osk2216 in live cell imaging experiments.

Article	Year
Return on Investment Analysis of Breast Cancer Screening and Downstaging in Egypt: Implications for Developing Countries. Value in health regional issues\| PubMed ID: 29626738	2018
: Python application for designing molecular beacons for live cell imaging of endogenous mRNAs. RNA (New York, N.Y.)\| PubMed ID: 30573696	2019

Omar S. Omar

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JoVE Journal Publications

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