an-unbiased-approach-of-sampling-tem-sections-in-neuroscience

An Unbiased Approach of Sampling TEM Sections in Neuroscience

Investigations of the ultrastructural features of neurons and their synapses are only possible with electron microscopy. Especially for comparative ...

Medical University of Graz

Formalin fixation prevents tissue autolysis by crosslinking proteins and changes tissue microstructure and MRI signal characteristics. Previous studies showed high variations in MR relaxation time constants of formalin fixed brain tissue, which has been attributed to the use of different formalin concentrations. Our investigations confirmed the influence of formalin concentration on relaxation times and unexpectedly revealed an influence of vendor specific formalin composition, which has not been investigated so far.

Effects of concentration and vendor specific composition of formalin on postmortem MRI of the human brain.

Current MRI techniques cannot reliably assess iron content in white matter due to the confounding diamagnetic effect of myelin. The purpose of this study was to validate with histology a novel iron mapping technique that uses the temperature dependency of the paramagnetic susceptibility in multiple sclerosis (MS) brains, where white matter has been reported to show significant variations in iron content.

Assessment of ferritin content in multiple sclerosis brains using temperature-induced R* changes.

This study explored whether autoregulatory shifts in cerebral blood volume induce susceptibility changes large enough to be depicted by quantitative susceptibility mapping. Eight healthy subjects underwent fast quantitative susceptibility mapping at 3T while lying down to slowly decrease mean arterial pressure. A linear relationship between mean arterial pressure and susceptibility was observed in cortical and subcortical structures, likely representing vessels involved in autoregulation. The slope of this relationship is assumed to indicate the extent of cerebral vascular compliance.

Article	Year
Effects of concentration and vendor specific composition of formalin on postmortem MRI of the human brain. Magnetic resonance in medicine\| PubMed ID: 28382642	2018
Assessment of ferritin content in multiple sclerosis brains using temperature-induced R* changes. Magnetic resonance in medicine\| PubMed ID: 28618066	2018
Quantitative Susceptibility Mapping to Assess Cerebral Vascular Compliance. AJNR. American journal of neuroradiology\| PubMed ID: 30679209	2019

Christoph Birkl

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