data-acquisition-analysis-brainstem-evoked-response-audiometry

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Brainstem evoked response audiometry (BERA) is of central relevance in the clinical neurophysiology. As other evoked potential (EP) techniques, such as ...

Federal Institute for Drugs and Medical Devices

This data article provides raw auditory evoked brainstem responses (ABRs) from controls and Ca2.3 transgenics, i.e. heterozygous Ca2.3 and Ca2.3 null mutants. Gender specific ABR recordings were performed in age-matched animals under ketamine/xylazine narcosis. Data presented here include ABRs upon both click and tone burst presentation in the increasing SPL mode using a commercially available ABR setup from Tucker Davis Technologies Inc. (TDT, USA). Detailed information is provided for the sound attenuating cubicle, electrical shielding, electrode parameters, stimulus characteristics and architecture, sampling rate, filtering processes and ABR protocol application during the course of data acquisition and recording. The later are important for subsequent analysis of click and tone burst related hearing thresholds, amplitude growth function and peak latencies. Raw data are available at MENDELEY DATA, DIO: 〈DOI:10.17632/g6ygz2spzx.1〉, URL: 〈https://data.mendeley.com/datasets/g6ygz2spzx/1〉).

Gender specific click and tone burst evoked ABR datasets from mice lacking the Ca2.3 R-type voltage-gated calcium channel.

Voltage-gated Ca channels (VGCCs) are of central relevance in regulating Ca influx into living cells. The low-voltage activated (LVA) Ca3 T-type Ca channels are widely distributed throughout the brain including the peripheral auditory system and ascending auditory tract. Their exact role in auditory information processing is still not fully understood. Within the LVA subgroup, Ca3.2 T-type Ca channels seem to be of special importance as qPCR revealed a steady increase in Ca3.2 transcript levels over age, e.g. in the cochlea and spiral ganglion neurons (SGN). Furthermore, pharmacological studies suggested an association between Ca3.2 expression and both age-related and noise-induced hearing loss. Given the potential functional relevance of Ca3.2 VGGCs in sensorineural hearing loss, we recorded gender specific auditory evoked brainstem responses (ABRs) upon both click and tone burst presentation. Here we present auditory brainstem response (ABR) data from Ca3.2, Ca3.2 and Ca3.2 mice from both genders which are of value for researchers who want to evaluate how Ca3.2 loss affects basic auditory parameters, e.g. click and tone burst based hearing thresholds, amplitude growth function and peak latencies.

Gender specific click and tone burst evoked ABR datasets from mice lacking the Ca3.2 T-type voltage-gated calcium channel.

Voltage-gated Ca channels (VGCCs) play key roles in auditory perception and information processing within the inner ear and brainstem. Pharmacological inhibition of low voltage-activated (LVA) T-type Ca channels is related to both age- and noise induced hearing loss in experimental animals and may represent a promising approach to the treatment of auditory impairment of various etiologies. Within the LVA Ca channel subgroup, Ca3.2 is the most prominently expressed T-type channel entity in the cochlea and auditory brainstem. Thus, we performed a complete gender specific click and tone burst based auditory brainstem response (ABR) analysis of Ca3.2 and Ca3.2 mice, including i.a. temporal progression in hearing loss, amplitude growth function and wave latency analysis as well as a cochlear qPCR based evaluation of other VGCCs transcripts. Our results, based on a self-programmed automated wavelet approach, demonstrate that both heterozygous and Ca3.2 null mutant mice exhibit age-dependent increases in hearing thresholds at 5 months of age. In addition, complex alterations in W amplitudes and latencies were detected that were not attributable to alterations in the expression of other VGCCs in the auditory tract. Our results clearly demonstrate the important physiological role of Ca3.2 VGCCs in the spatiotemporal organization of auditory processing in young adult mice and suggest potential pharmacological targets for interventions in the future.

Article	Year
Gender specific click and tone burst evoked ABR datasets from mice lacking the Ca2.3 R-type voltage-gated calcium channel. Data in brief\| PubMed ID: 30456242	2018
Gender specific click and tone burst evoked ABR datasets from mice lacking the Ca3.2 T-type voltage-gated calcium channel. BMC research notes\| PubMed ID: 30894204	2019
Ca3.2 T-Type Calcium Channels Are Physiologically Mandatory for the Auditory System. Neuroscience\| PubMed ID: 31029730	2019

Muhammad Imran Arshaad

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