a-galleria-mellonella-oral-administration-model-to-study-commensal

A <em>Galleria mellonella</em> Oral Administration Model to Study Commensal-Induced Innate Immune Responses

The investigation of the immunogenic potential of commensal bacteria on the host immune system is one essential component when studying intestinal ...

University of Tübingen

An important question: Which LPS do you use?

The larvae of the greater wax moth, , are pests of active beehives. In infection biology, these larvae are playing a more and more attractive role as an invertebrate host model. Here, we report on the first genome sequence of .

Genome Sequence of  (Greater Wax Moth).

Insects and mammals share evolutionary conserved innate immune responses to maintain intestinal homeostasis. We investigated whether the larvae of the greater wax moth  may be used as an experimental organism to distinguish between symbiotic  and pathobiotic , which are mammalian intestinal commensals. Oral application of the symbiont or pathobiont to  resulted in clearly distinguishable innate immune responses that could be verified by analyzing similar innate immune components in mice  and . The differential innate immune responses were initiated by the recognition of bacterial components  pattern recognition receptors. The pathobiont detection resulted in increased expression of reactive oxygen and nitrogen species related genes as well as antimicrobial peptide gene expression. In contrast, the treatment/application with symbiotic bacteria led to weakened immune responses in both mammalian and insect models. As symbionts and pathobionts play a crucial role in development of inflammatory bowel diseases, we hence suggest  as a future replacement organism in inflammatory bowel disease research.

Article	Year
An important question: Which LPS do you use? Virulence\| PubMed ID: 28891747	2017
Genome Sequence of (Greater Wax Moth). Genome announcements\| PubMed ID: 29326202	2018
: A Novel Invertebrate Model to Distinguish Intestinal Symbionts From Pathobionts. Frontiers in immunology\| PubMed ID: 30283451	2018

Anna Lange

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