differentiation-capacity-human-aortic-perivascular-adipose-progenitor

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

Adipose tissue is a rich source of multi-potent mesenchymal stem cells (MSC) capable of differentiating into osteogenic, adipogenic, and chondrogenic ...

Maine Medical Center Research Institute

The most important lessons I learned in training.

The adult human heart contains a subpopulation of highly proliferative cells. The role of ErbB receptors in these cells has not been studied. From human left ventricular (LV) epicardial biopsies, we isolated highly proliferative cells (eHiPC) to characterize the cell surface expression and function of ErbB receptors in the regulation of cell proliferation and phenotype. We found that human LV eHiPC express all four ErbB receptor subtypes. However, the expression of ErbB receptors varied widely among eHiPC isolated from different subjects. eHiPC with higher cell surface expression of ErbB2 reproduced the phenotype of endothelial cells and were characterized by endothelial cell-like functional properties. We also found that EGF/ErbB1 induces VEGFR2 expression, while ligands for both ErbB1 and ErbB3/4 induce expression of Tie2. The number of CD31CD45 endothelial cells is higher in LV biopsies from subjects with high ErbB2 (ErbB2) eHiPC compared to low ErbB2 (ErbB2) eHiPC. These findings have important implications for potential strategies to increase the efficacy of cell-based revascularization of the injured heart, through promotion of an endothelial phenotype in cardiac highly proliferative cells.

ErbB2 promotes endothelial phenotype of human left ventricular epicardial highly proliferative cells (eHiPC).

Perivascular adipose tissue (PVAT) surrounds blood vessels and regulates vascular tone through paracrine secretion of cytokines. During conditions promoting cardiometabolic dysfunction, such as obesity, cytokine secretion is altered towards a proinflammatory and proatherogenic profile. Despite the clinical implications for cardiovascular disease, studies addressing the biology of human PVAT remain limited. We are interested in characterizing the resident adipose progenitor cells (APCs) because of their potential role in PVAT expansion during obesity. We also focused on proteins regulating paracrine interactions, including the small GTPase Rab27a, which regulates protein trafficking and secretion.

Rab27a Regulates Human Perivascular Adipose Progenitor Cell Differentiation.

A patient's hemoglobin (Hb) A level, regardless of diabetic status, is a measure of glycemic control. Studies have found it is an independent predictor of short-term death in patients undergoing coronary artery bypass grafting (CABG). In this study, we used preoperative HbA to assess whether levels are associated with short-term and long-term survival after CABG.

Article	Year
The most important lessons I learned in training. The Journal of thoracic and cardiovascular surgery\| PubMed ID: 28528716	2017
ErbB2 promotes endothelial phenotype of human left ventricular epicardial highly proliferative cells (eHiPC). Journal of molecular and cellular cardiology\| PubMed ID: 29291395	2018
Rab27a Regulates Human Perivascular Adipose Progenitor Cell Differentiation. Cardiovascular drugs and therapy\| PubMed ID: 30105417	2018
Intensity of Glycemic Control Affects Long-Term Survival After Coronary Artery Bypass Graft Surgery. The Annals of thoracic surgery\| PubMed ID: 30273572	2019
Successful rebuilding after disaster, even in the heart, starts with infrastructure. Journal of thoracic disease\| PubMed ID: 30631583	2018

Michael Robich

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