use-of-human-perivascular-stem-cells-for-bone-regeneration

Use of Human Perivascular Stem Cells for Bone Regeneration

Human perivascular stem cells (PSCs) can be isolated in sufficient numbers from multiple tissues for purposes of skeletal tissue engineering1-3. PSCs are ...

School of Dentistry, UCLA

There has been a recent increase in our understanding in the isolation, culture, and differentiation of mesenchymal stem cells (MSCs). Concomitantly, the availability of MSCs has increased, with cells now commercially available, including human MSCs from adipose tissue and bone marrow. Despite an increased understanding of MSC biology and an increase in their availability, standardization of techniques for adipogenic differentiation of MSCs is lacking. The following review will explore the variability in adipogenic differentiation in vitro, specifically in 3T3-L1 and primary MSCs derived from both adipose tissue and bone marrow. A review of alternative methods of adipogenic induction is also presented, including the use of specific peroxisome proliferator-activated receptor-gamma agonists as well as bone morphogenetic proteins. Finally, we define a standard, commonly used adipogenic differentiation medium in the hopes that this will be adopted for the future standardization of laboratory techniques--however, we also highlight the essentially arbitrary nature of this decision. With the current, rapid pace of electronic publications, it becomes imperative for standardization of such basic techniques so that interlaboratory results may be easily compared and interpreted.

Article	Year
Ophthalmic plastic and reconstructive surgery\| PubMed ID: 19300160	2009
Cornea\| PubMed ID: 19421037	2009
The Journal of biological chemistry\| PubMed ID: 21282107	2011
Current methods of adipogenic differentiation of mesenchymal stem cells. Stem cells and development\| PubMed ID: 21526925	2011
Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association\| PubMed ID: 21889867	2011
Cell and tissue research\| PubMed ID: 22081226	2011
Structure (London, England : 1993)\| PubMed ID: 22483109	2012
Journal of orthopaedic trauma\| PubMed ID: 22487905	2012
The American journal of sports medicine\| PubMed ID: 22495145	2012

Virginia Nguyen

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