an-vitro-batch-culture-model-to-estimate-effects-interventional

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

The emerging role of the gut microbiome in several human diseases demands a breakthrough of new tools, techniques and technologies. Such improvements are ...

Isfahan university of Technology

ISFAHAN UNIVERSITY OF TECHNOLOGY

This study aimed to optimize the suitable drying method to obtain high yield of polysaccharides from mulberry leaves and to determine their structural characterization and antioxidant activities. The effects of three different drying methods such as air dried, hot air dried (55 °C, 65 °C & 75 °C) and freeze dried on the physicochemical and antioxidant properties of mulberry leaves polysaccharides were studied using gas chromatography, high performance gel permeation chromatography, Fourier transform infrared spectroscopy, scanning electron micrography and antioxidant assays. Results revealed that pre-treatment remarkably influenced the changes in their physicochemical and antioxidant properties. In comparison with the other drying techniques, freeze dried polysaccharides showed more rough morphologies and significant antioxidant property. The yield of polysaccharides from the freeze dried sample was about 28.88% higher than the yield of hot air dried sample. The MDA activity of freeze dried sample was about 95.45%. Overall, the results suggested that the freeze drying technique was the appropriate method to extract polysaccharides from mulberry leaves that offered significant biological properties.

Effect of different drying methods on the physicochemical properties and antioxidant activities of mulberry leaves polysaccharides.

The prevalence of metabolic diseases including obesity, diabetes, cardiovascular diseases, hypertension and cancer has evolved into a global epidemic over the last century. The rate of these disorders is continuously rising due to the lack of effective preventative and therapeutic strategies. This warrants for the development of novel strategies that could help in the prevention, treatment and/ or better management of such disorders. Although the complex pathophysiology of these metabolic diseases is one of the major hurdles in the development of preventive and/or therapeutic strategies, there are some factors that are or can speculated to be more effective to target than others. Recently, gut microbiome has emerged as one of the major contributing factors in metabolic diseases, and developing positive modulators of gut microbiota is being considered to be of significant interest. Natural non-digestible polysaccharides from plants and food sources are considered potent modulators of gut microbiome that can feed certain beneficial microbes in the gut. This has led to an increased interest in the isolation of novel bioactive polysaccharides from different plants and food sources and their application as functional components to modulate the gut microbiome composition to improve host's health including metabolism. Therefore, polysaccharides, as prebiotics components, are being speculated to confer positive effects in managing metabolic diseases like obesity and diabetes. In this review article, we summarize some of the most common polysaccharides from plants and food that impact metabolic health and discuss why and how these could be helpful in preventing or ameliorating metabolic diseases such as obesity, type 2 diabetes, hypertension and dyslipidemia.

Dietary Polysaccharides in the Amelioration of Gut Microbiome Dysbiosis and Metabolic Diseases.

Role of gut microbiome in obesity and type 2 diabetes (T2D) became apparent from several independent studies indicating that gut microbiome modulators like prebiotics may improve microbiome perturbations (dysbiosis) to ameliorate metabolic derangements. We herein isolate water soluble, nondigestible polysaccharides from five plant-based foods (acorn, quinoa, sunflower, pumpkin seeds and sago) and assess their impact on human fecal microbiome and amelioration of high-fat-diet (HFD)-induced obesity/T2D in mice. During polysaccharide isolation, purification, biochemical and digestion resistance characterization, and fermentation pattern by human fecal microbiome, we select acorn- and sago-derived prebiotics (on the basis of relatively higher purity and yield and lower protein contamination) and examine their effects in comparison to inulin. Prebiotics treatments in human fecal microbiome culture system not only preserve microbial diversity but also appear to foster beneficial bacteria and short-chain fatty acids (SCFAs). Feeding of acorn- and sago-derived prebiotics ameliorates HFD-induced glucose intolerance and insulin resistance in mice, with effects comparatively superior to those seen in inulin-fed mice. Feeding of both of novel prebiotics as well as inulin increases SCFAs levels in the mouse gut. Interestingly, gut hyperpermeability and mucosal inflammatory markers were significantly reduced upon prebiotics feeding in HFD-fed mice. Hypothalamic energy signaling in terms of increased expression of pro-opiomelanocortin was also modulated by prebiotics administration. Results demonstrate that these (and/or such) novel prebiotics can ameliorate HFD-induced defects in glucose metabolism via positive modulation of gut-microbiome-brain axis and hence could be useful in preventing/treating diet-induced obesity/T2D.

Article	Year
Effect of different drying methods on the physicochemical properties and antioxidant activities of mulberry leaves polysaccharides. International journal of biological macromolecules\| PubMed ID: 30098363	2018
Dietary Polysaccharides in the Amelioration of Gut Microbiome Dysbiosis and Metabolic Diseases. Obesity & control therapies : open access\| PubMed ID: 30474051	2017
Prebiotics from acorn and sago prevent high-fat-diet-induced insulin resistance via microbiome-gut-brain axis modulation. The Journal of nutritional biochemistry\| PubMed ID: 30831458	2019

Sabihe Soleimanian-Zad

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