the-use-mouse-mammary-tumor-cells-an-vitro-invasion-assay-as-measure

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

The in vitro invasion assay uses a protein-rich matrix in a Boyden chamber to measure the ability of cultured cells to pass through the matrix and a ...

Villanova University

Determining the abundance of phospholipids and neutral lipids in cellular membranes is paramount to understanding their biological functions. Many lipid-modifying enzymes have yet to be characterized due to limitations in substrate-product measurements and purification of membrane-bound enzymes. The method described here uses radiolabeled phospholipid substrates and cell-purified organelles to quantify phospholipid metabolism using thin-layer chromatography. This assay has the benefits of being specific and adaptable for numerous applications and systems.

The Role of Lysophospholipid Acyltransferases in the Golgi Complex.

The Zc3h8 gene encodes a protein with three zinc finger motifs in the C-terminal region. The protein has been identified as a component of the Little Elongation Complex, involved in transcription of small nuclear RNAs. ZC3H8 is overexpressed in a number of human and mouse breast cancer cell lines, and elevated mRNA levels are associated with a poorer prognosis for women with breast cancer.

Article	Year
The Role of Lysophospholipid Acyltransferases in the Golgi Complex. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 27632011	2016
Regulation of the oncogenic phenotype by the nuclear body protein ZC3H8. BMC cancer\| PubMed ID: 30041613	2018

John A. Schmidt

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