sample-preparation-for-probe-electrospray-ionization-mass-spectrometry

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Mass spectrometry (MS) is a powerful tool in analytical chemistry because it provides very accurate information about molecules, such as mass-to-charge ...

University of Yamanashi School of Medicine

Atherosclerotic diseases are the leading cause of death worldwide. Biomarkers of atherosclerosis are required to monitor and prevent disease progression. While mass spectrometry is a promising technique to search for such biomarkers, its clinical application is hampered by the laborious processes for sample preparation and analysis.

Detection of potential new biomarkers of atherosclerosis by probe electrospray ionization mass spectrometry.

Real-time and in-situ mass-spectrometry analyses of living animal and biological sample were performed using a novel remote sampling electrospray ionization (RS-ESI) probe. Unlike conventional ESI, in which injection or syringe loading is required for sample introduction, the RS-ESI probe ionizes the samples when the sampling capillary is in contact with the sample. As the sampling capillary is electrically held at ground potential, the safety of the animal and operator is assured. The liquid sample is aspirated to the ESI emitter at the other end of the capillary by the Venturi effect. Subsequently, the electrospray is generated when a high voltage is applied to the counter electrode placed inside the ion source chamber. The probe unit is attached to the mass spectrometer with a long flexible tube and its position can be freely manipulated during the analysis. In this report, we demonstrate a real-time analysis of a living mouse liver and an automatic analysis of 138 serum samples using this new technique.

Article	Year
Detection of potential new biomarkers of atherosclerosis by probe electrospray ionization mass spectrometry. Metabolomics : Official journal of the Metabolomic Society\| PubMed ID: 30830369	2018
Real-time analysis of living animals and rapid screening of human fluid samples using remote sampling electrospray ionization mass spectrometry. Journal of pharmaceutical and biomedical analysis\| PubMed ID: 31096096	2019

Kentaro Yoshimura

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