Piezo-ICSI for Human Oocytes

Since the first successful pregnancies achieved by intracytoplasmic sperm injection (ICSI) were reported, ICSI has become an essential technique in ...

Kameda Medical Center

Kameda IVF Clinic Makuhari

Tokyo Medical and Dental University

The purposes of the present study are to assess the clinical efficiency of Piezo-intracytoplasmic sperm injection (ICSI) and to improve the Piezo-ICSI method for human oocytes.

Clinical efficiency of Piezo-ICSI using micropipettes with a wall thickness of 0.625 μm.

Erratum to: J Assist Reprod Genet (2015) 32:1827–1833, DOI 10.1007/s10815-015-0597-9. The authors would like to apologize for accidentally making a mistake in the inner and outer diameter calculation of the Piezo-ICSI micropipettes.

Erratum to: Clinical efficiency of Piezo-ICSI using micropipettes with a wall thickness of 0.625 μm.

The objective of this study was to investigate the effect of the head-first or tail-first injection of sperm into the cytoplasm by Piezo-ICSI (PICSI) on oocyte survival, fertilization, embryo development and implantation ability in humans.

Effects of sperm direction in Piezo-ICSI on oocyte survival, fertilization, embryo development and implantation ability in humans: A preliminary study.

A husband and his wife, both 34 years old, consulted our clinic because of primary infertility. Sperm analysis revealed that the sperm concentration, motility, and progressive motility were (42.8±22.8)×10/mL, 23.3%±12.2%, and 12.9%±6.1%, respectively. Based on Krugar strict morphology criteria, 100% of the sperm were teratozoospermic, with 7.9% DNA fragmentation index. Observation of the sperm under a transmission electron microscope revealed that most parts of the fibrous sheath (FS) surrounding the tails of the sperm were missing from midway through the principal piece to the end piece, although the sperm's heads, necks and midpieces were morphologically normal. To collect oocytes, the gonadotropin-releasing hormone antagonist protocol was carried out, and 7 oocytes were retrieved. Intracytoplasmic sperm injection (ICSI) was performed for all the teratozoospermic sperm. Of the 7 oocytes, 3 were fertilized, and one 8-cell embryo and 2 expanded blastocysts were vitrified. Although repeated transfers of expanded blastocysts resulted in no implantation, one 8-cell embryo transfer in a hormone replacement therapy cycle led to pregnancy. The pregnancy using an 8-cell vitrified embryo resulted in the delivery of a healthy female baby at 38 weeks of gestation. No congenital malformations were found until 28 days after birth. Our results demonstrated that healthy birth could be achieved following the transfer of an embryo derived from ICSI using teratozoospermic sperm exhibiting the dysplasia of the fibrous sheath (DFS). Furthermore, while the previous reports on DFS have not investigated male infertility, we evaluated sperms from various aspects such as Kruger sperm function test, chromatin dispersion test, electron microscopy findings, time-lapse images of the obtained embryos, and concluded that ICSI could be desirable as a treatment policy for DFS.

Successful pregnancy and delivery achieved following intracytoplasmic sperm injection using teratozoospermic sperm exhibiting marked dysplasia of the fibrous sheath: a case report.

Article	Year
Clinical efficiency of Piezo-ICSI using micropipettes with a wall thickness of 0.625 μm. Journal of assisted reproduction and genetics\| PubMed ID: 26489413	2015
Erratum to: Clinical efficiency of Piezo-ICSI using micropipettes with a wall thickness of 0.625 μm. Journal of assisted reproduction and genetics\| PubMed ID: 26922353	2016
Effects of sperm direction in Piezo-ICSI on oocyte survival, fertilization, embryo development and implantation ability in humans: A preliminary study. The journal of obstetrics and gynaecology research\| PubMed ID: 29992667	2018
Successful pregnancy and delivery achieved following intracytoplasmic sperm injection using teratozoospermic sperm exhibiting marked dysplasia of the fibrous sheath: a case report. Translational andrology and urology\| PubMed ID: 32420187	2020

Kenichiro Hiraoka

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