Xianyong Liu

National Animal Protozoa Laboratory, China Agricultural University

Affiliated withChina Agricultural University

Research Area

Biography

Xianyong Liu is an Associate Professor in the Veterinary Parasitology Department, College of Veterinary Medicine of China Agricultural University (CAU) in Beijing. He received his undergraduate degree and a Ph.D. from this university.

During Dr. Xianyong’s training he optimized the protocol for the development of transient and stable transfection of Eimeria tenella, a protozoa causing cecal coccidiosis in chicken. As a post-doctoral fellow (2012 to 2013) in Prof James Lok’s lab at Pathobiology Department, University of Pennsylvania, US, he tried the TALEN tool in Strongyloides stecoralis.

Dr. Xianyong was enrolled as a lecturer at CAU. His research program encompasses eimerian parasites of chicken and rabbits to develop molecular tools for the genetic manipulation of these parasites, as well as to develop vaccines based on the genetically modified eimerian parasites.

JoVE Journal Publications

ArticleTotal : 2	Year
Publication title Cited by 9	2020
Publication title	2026

Other Publications

Article	Year
Transient transfection of Eimeria tenella using yellow or red fluorescent protein as a marker. Molecular and biochemical parasitology\| PubMed ID: 17399806	2007
Restriction enzyme-mediated transfection improved transfection efficiency in vitro in Apicomplexan parasite Eimeria tenella. Molecular and biochemical parasitology\| PubMed ID: 18606196	2008
Stable transfection of Eimeria tenella: constitutive expression of the YFP-YFP molecule throughout the life cycle. International journal for parasitology\| PubMed ID: 18718473	2009
Dynamic development of parasitophorous vacuole of Eimeria tenella transfected with the yellow fluorescent protein gene fused to different signal sequences from apicomplexan parasites. Parasitology research\| PubMed ID: 18815811	2009
Transfection of Eimeria and Toxoplasma using heterologous regulatory sequences. International journal for parasitology\| PubMed ID: 19379753	2009
Co-expression of reporter genes in the widespread pathogen Eimeria tenella using a double-cassette expression vector strategy. International journal for parasitology\| PubMed ID: 21550346	2011
Transgenic Eimeria tenella expressing enhanced yellow fluorescent protein targeted to different cellular compartments stimulated dichotomic immune responses in chickens. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 21876035	2011
Large-scale survey of the prevalence of Eimeria infections in domestic rabbits in China. Parasitology research\| PubMed ID: 21972072	2012
High transfection efficiency of porcine peripheral blood T cells via nucleofection. Veterinary immunology and immunopathology\| PubMed ID: 22055481	2011
Molecular cloning and characterization of the full-length cDNA encoding the porcine CD28. Veterinary immunology and immunopathology\| PubMed ID: 22510524	2012
piggyBac transposon-mediated transgenesis in the apicomplexan parasite Eimeria tenella. PloS one\| PubMed ID: 22768223	2012
Plasmid DNA could be delivered into Eimeria maxima unsporulated oocyst with gene gun system. Acta veterinaria Hungarica\| PubMed ID: 23160025	2012
Development of transgenic lines of Eimeria tenella expressing M2e-enhanced yellow fluorescent protein (M2e-EYFP). Veterinary parasitology\| PubMed ID: 23298569	2013
Expression of Toxoplasma gondii dense granule protein7 (GRA7) in Eimeria tenella. Parasitology research\| PubMed ID: 23371499	2013
Molecular cloning and protein characterization of swine 4-1BB. Veterinary immunology and immunopathology\| PubMed ID: 23453329	2013
Interferon-γ enzyme-linked immunosorbent spot assay as a tool to study T cell responses to Eimeria tenella infection in chickens. Poultry science\| PubMed ID: 23776262	2013
An Eimeria vaccine candidate based on Eimeria tenella immune mapped protein 1 and the TLR-5 agonist Salmonella typhimurium FliC flagellin. Biochemical and biophysical research communications\| PubMed ID: 24076159	2013
CDR3 analysis of TCR Vβ repertoire of CD8⁺ T cells from chickens infected with Eimeria maxima. Experimental parasitology\| PubMed ID: 24801021	2014
Molecular analysis of single oocyst of Eimeria by whole genome amplification (WGA) based nested PCR. Experimental parasitology\| PubMed ID: 24996066	2014
Ethanol and isopropanol trigger rapid egress of intracellular Eimeria tenella sporozoites. Parasitology research\| PubMed ID: 25407127	2015
Protective immunity against Eimeria tenella infection in chickens induced by immunization with a recombinant C-terminal derivative of EtIMP1. Veterinary immunology and immunopathology\| PubMed ID: 25464823	2014
Exploring early and late Toxoplasma gondii strain RH infection by two-dimensional immunoblots of chicken immunoglobulin G and M profiles. PloS one\| PubMed ID: 25803039	2015
Immunogenicity and protective efficacy of an Eimeria vaccine candidate based on Eimeria tenella immune mapped protein 1 and chicken CD40 ligand. Veterinary parasitology\| PubMed ID: 25840621	2015
Transgenic Eimeria mitis expressing chicken interleukin 2 stimulated higher cellular immune response in chickens compared with the wild-type parasites. Frontiers in microbiology\| PubMed ID: 26082759	2015
Nitric oxide stimulates early egress of Toxoplasma gondii tachyzoites from Human foreskin fibroblast cells. Parasites & vectors\| PubMed ID: 26264067	2015
Interferon-Gamma Release Assay: An Effective Tool to Detect Early Toxoplasma gondii Infection in Mice. PloS one\| PubMed ID: 26378802	2015
Immunoproteomic technology offers an extraordinary diagnostic approach for Toxoplasma gondii infection. Journal of microbiological methods\| PubMed ID: 26415530	2015
From the Macro to the Micro: Gel Mapping to Differentiate between Sporozoites of Two Immunologically Distinct Strains of Eimeria maxima (Strains M6 and Guelph). PloS one\| PubMed ID: 26641262	2015
Chicken IgY Fc expressed by Eimeria mitis enhances the immunogenicity of E. mitis. Parasites & vectors\| PubMed ID: 27000834	2016
"Self-cleaving" 2A peptide from porcine teschovirus-1 mediates cleavage of dual fluorescent proteins in transgenic Eimeria tenella. Veterinary research\| PubMed ID: 27352927	2016
Survey of coccidial infection of rabbits in Sichuan Province, Southwest China. SpringerPlus\| PubMed ID: 27386319	2016
Transgenic Eimeria tenella as a vaccine vehicle: expressing TgSAG1 elicits protective immunity against Toxoplasma gondii infections in chickens and mice. Scientific reports\| PubMed ID: 27387302	2016
Calcium-dependent microneme protein discharge and in vitro egress of Eimeria tenella sporozoites. Experimental parasitology\| PubMed ID: 27680997	2016
High pathogenicity and strong immunogenicity of a Chinese isolate of Eimeria magna Pérard, 1925. Parasitology international\| PubMed ID: 28132801	2017
Transgenic Pérard, 1925 Displays Similar Parasitological Properties to the Wild-type Strain and Induces an Exogenous Protein-Specific Immune Response in Rabbits ( L.). Frontiers in immunology\| PubMed ID: 28167939	2017
Mucosal IgA and IFN-γ CD8 T cell immunity are important in the efficacy of live Salmonella enteria serovar Choleraesuis vaccines. Scientific reports\| PubMed ID: 28406162	2017
A new species of Eimeria (Apicomplexa: Eimeriidae) from Californian rabbits in Hebei Province, China. Parasitology international\| PubMed ID: 28663008	2017
Increased Neutralizing Antibody Production and Interferon-γ Secretion in Response to Porcine Reproductive and Respiratory Syndrome Virus Immunization in Genetically Modified Pigs. Frontiers in immunology\| PubMed ID: 28955336	2017
Improved Cytotoxic T Lymphocyte Responses to Vaccination with Porcine Reproductive and Respiratory Syndrome Virus in 4-1BB Transgenic Pigs. Frontiers in immunology\| PubMed ID: 29326720	2017
A Novel Vaccine Delivery Model of the Apicomplexan Expressing Antigen Protects Chickens against Infection of the Two Parasites. Frontiers in immunology\| PubMed ID: 29375584	2017
An optimized DNA extraction method for molecular identification of coccidian species. Parasitology research\| PubMed ID: 29396674	2018
Influence of Eimeria falciformis Infection on Gut Microbiota and Metabolic Pathways in Mice. Infection and immunity\| PubMed ID: 29440368	2018
Transgenic Eimeria tenella Expressing Profilin of Eimeria maxima Elicits Enhanced Protective Immunity and Alters Gut Microbiome of Chickens. Infection and immunity\| PubMed ID: 29967093	2018
Eimeria tenella infection perturbs the chicken gut microbiota from the onset of oocyst shedding. Veterinary parasitology\| PubMed ID: 30105975	2018
Comparative transcriptome analysis of Eimeria maxima (Apicomplexa: Eimeriidae) suggests DNA replication activities correlating with its fecundity. BMC genomics\| PubMed ID: 30249186	2018
Stable transfection of Eimeria necatrix through nucleofection of second generation merozoites. Molecular and biochemical parasitology\| PubMed ID: 30658178	2019
Selection and identification of a precocious line of Eimeria intestinalis with enlarged oocysts and deletion of one generation of schizogony. Parasitology research\| PubMed ID: 30694415	2019
Inflammasome activation restrains the intracellular Neospora caninum proliferation in bovine macrophages. Veterinary parasitology\| PubMed ID: 30981301	2019
Transcriptional profiling of innate immune responses in sheep PBMCs induced by Haemonchus contortus soluble extracts. Parasites & vectors\| PubMed ID: 31023355	2019
Identification of candidate antigens by 2-DE Immunoblotting for diagnosis of Toxoplasma gondii infection in chickens and rabbits. Experimental parasitology\| PubMed ID: 31299265	2019
Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection. Parasites & vectors\| PubMed ID: 31300007	2019
Selection and characterization of a precocious line of Eimeria media. Parasitology research\| PubMed ID: 31407118	2019
Immune protection provided by a precocious line trivalent vaccine against rabbit Eimeria. Veterinary parasitology\| PubMed ID: 31639571	2019