evaluation-cognitive-performance-hypertensive-patients-with-silent

Evaluation of the Cognitive Performance of Hypertensive Patients with Silent Cerebrovascular Lesions

Evidence accumulated from the last decade has proven that silent cerebrovascular lesions (SCLs) and their underlying pathogenic processes contribute to ...

The First Affiliated Hospital of Wenzhou Medical University

The University of Hong Kong

A PRiME (process, robustness, improvements, matrix effects, ease of use) pass-through cleanup procedure was developed for the extraction and purification of silychristins A and B, silybins A and B, isosilybins A and B, and silydianin in Silybum marianum. After optimizing the extracting solvent types and the sample loading volume, the crude extract was diluted to 3 mL with 95% acetonitrile and then loaded on the PRiME cartridge. The eluate was analyzed by ultra-performance liquid chromatography and tandem mass spectrometry (UPLC-MS/MS). All the target analytes were deprotonated as [M-H] at m/z 481 by conducting collision-induced dissociation (CID), and the major fragment ions were m/z 463 ([M-HO-H]), 453 ([M-CO-H]), 355 ([M-CHO-H]), 301 ([M-CO-H]), and 179 ([CHO]). Afterwards, this method was validated in terms of linearity (R > 0.9990), intra-day precision (1.02%-3.79%), inter-day precision (1.59%-4.87%), sensitivity (LOD ≤ 0.45 μg·kg and LOQ ≤ 1.50 μg·kg), and recovery (76.9-103.4%, RSD < 8.90%). Finally, the proposed protocol was successfully applied to eight batches of S. marianum samples. The total content of the seven active compounds varied amongst the batches from different places of origin.

PRiME pass-through purification of lignans in Silybum marianum and UPLC-MS/MS analysis.

 A sensitive and selective ultra performance LC-MS/MS (UPLC-MS/MS) method was developed to investigate the pharmacokinetics of rosavin as a potential adaptogenic drug isolated from  in rat plasma with salidroside as an internal standard.  Chromatographic separation was performed on a UPLC HSS T3 column (1.8 μm, 100 mm × 2.1 mm) with gradient elution. Multiple reaction monitoring was employed for MS analysis. Rosavin and salidroside were determined with multiple reaction monitoring-ion transitions  427.2 → 293.1 and  299.1 → 119.1, respectively.  The validated UPLC-MS/MS method showed a satisfied linear range in 5-5000 ng/ml and was successfully applied for the pharmacokinetic study of rosavin in the rat after intravenous and gavage administration.

Pharmacokinetic study of rosavin in rat plasma with ultra performance LC-MS/MS after intravenous and gavage administration.

Silent cerebrovascular lesions (SCLs) and their underlying pathology are now recognized as important causes of cognitive impairment in the elderly. However, the distinct profile of cognitive deficits associated with each type of SCLs remains unclear.

Distinct profiles of cognitive impairment associated with different silent cerebrovascular lesions in hypertensive elderly Chinese.

Malnutrition is prevalent among individuals with acute ischaemic stroke (AIS) and may worsen clinical outcomes. There is no consensus on the best tool for nutritional screening in this population. The present study compared four screening tools and one diagnostic tool in terms of their prognostic significance in predicting short-term and long-term outcomes in AIS patients.

Article	Year
PRiME pass-through purification of lignans in Silybum marianum and UPLC-MS/MS analysis. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences\| PubMed ID: 30015310	2018
Pharmacokinetic study of rosavin in rat plasma with ultra performance LC-MS/MS after intravenous and gavage administration. Bioanalysis\| PubMed ID: 31094215	2019
Distinct profiles of cognitive impairment associated with different silent cerebrovascular lesions in hypertensive elderly Chinese. Journal of the neurological sciences\| PubMed ID: 31284183
Comparing the prognostic significance of nutritional screening tools and ESPEN-DCM on 3-month and 12-month outcomes in stroke patients. Clinical nutrition (Edinburgh, Scotland)\| PubMed ID: 33221053	2020

Manman Zhang

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