monitoring-protein-rna-interaction-dynamics-vivo-at-high-temporal

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC

The interaction between RNA-binding proteins (RBPs) and their RNA substrates exhibits fluidity and complexity. Within its lifespan, a single RNA can be ...

Aarhus University

The Microprocessor complex catalyzes the first step of miRNA biogenesis in the nucleus of mammalian cells. The minimal catalytically active complex is formed by two essential factors, the dsRNA binding protein DGCR8, and the RNase III endonuclease Drosha. Importantly, several co-factors can associate to this complex and modulate the cleavage and binding efficiency of this complex, in a positive or negative manner. Here, we describe a simple method for purification of DGCR8 and Drosha coupled to mass spectrometry or western blot which allows robust identification of unknown associated factors. This approach has recently revealed the presence of a new DGCR8-dependent, Drosha-independent complex involved in RNA turnover.

Ross A. Cordiner

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