setup-capillary-electrophoresis-inductively-coupled-plasma-mass

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))

Dyshomeostasis of iron metabolism is accounted in the pathophysiological framework of numerous diseases, including cancer and several neurodegenerative ...

University Medical Center Göttingen (UMG)

 Angiosarcomas are soft tissue sarcomas with endothelial differentiation and vasoformative capacity. Most angiosarcomas show strong constitutive expression of the endothelial adhesion receptor CD31/PECAM-1 pointing to an important role of this molecule. However, the biological function of CD31 in angiosarcomas is unknown. The expression levels of CD31 in angiosarcoma cells and its effects on cell viability, colony formation, and chemoresistance were evaluated in human angiosarcoma clinical samples and in cell lines through isolation of CD31 and CD31 cell subsets. The redox-regulatory CD31 function linked to YAP signaling was determined using a CD31-blocking antibody and siRNA approach and was further validated in CD31-knockout endothelial cells. We found that most angiosarcomas contain a small CD31 cell population. CD31 cells had lost part of their endothelial properties and were more tumorigenic and chemoresistant than CD31 cells due to more efficient reactive oxygen species (ROS) detoxification. Active downregulation of CD31 resulted in loss of endothelial tube formation, nuclear accumulation of YAP, and YAP-dependent induction of antioxidative enzymes. Addition of pazopanib, a known enhancer of proteasomal YAP degradation resensitized CD31 cells for doxorubicin resulting in growth suppression and induction of apoptosis. Human angiosarcomas contain a small aggressive CD31 population that have lost part of their endothelial differentiation programs and are more resistant against oxidative stress and DNA damage due to intensified YAP signaling. Our finding that the addition of YAP inhibitors can resensitize CD31 cells toward doxorubicin may aid in the rational development of novel combination therapies to treat angiosarcomas. .

CD31 Expression Determines Redox Status and Chemoresistance in Human Angiosarcomas.

 Validation of the Endothelial Cell Receptor GRP78 in a Case of Rhinocerebral Mucormycosis.

Manganese (Mn) is an essential trace element that is naturally found in the environment and is necessary as a cofactor for many enzymes and is important in several physiological processes that support development, growth, and neuronal function. However, overexposure to Mn may induce neurotoxicity and may contribute to the development of Alzheimer's disease (AD) and Parkinson's disease (PD). The present review aims to provide new insights into the involvement of Mn in the etiology of AD and PD. Here, we discuss the critical role of Mn in the etiology of these disorders and provide a summary of the proposed mechanisms underlying Mn-induced neurodegeneration. In addition, we review some new therapy options for AD and PD related to Mn overload.

Article	Year
CD31 Expression Determines Redox Status and Chemoresistance in Human Angiosarcomas. Clinical cancer research : an official journal of the American Association for Cancer Research\| PubMed ID: 29084920	2018
Validation of the Endothelial Cell Receptor GRP78 in a Case of Rhinocerebral Mucormycosis. Antimicrobial agents and chemotherapy\| PubMed ID: 29483124	2018
New Insights on the Role of Manganese in Alzheimer's Disease and Parkinson's Disease. International journal of environmental research and public health\| PubMed ID: 31546716	2019

Vivek Venkataramani

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