visualizing-diffusional-dynamics-gold-nanorods-on-cell-membrane-using

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Analyzing the diffusional dynamics of nanoparticles on cell membrane plays a significant role in better understanding the cellular uptake process and ...

Tsinghua University

Flagellated bacteria move collectively in a swirling pattern on agar surfaces immersed in a thin layer of viscous "swarm fluid," but the role of this fluid in mediating the cooperation of the bacterial population is not well understood. Herein, we use gold nanorods (AuNRs) as single particle tracers to explore the spatiotemporal structure of the swarm fluid. Individual AuNRs are moving in a plane of ∼2 μm above swarms, traveling for long distances in high speed without interferences from bacterial movements. The particles are lifted and transported by collective mixing of small vortices around bacteria during localized clustering and de-clustering of motile cells. Their motions fit the Lévy walk model, revealing efficient fluidic flows above the swarms. These flows provide obstacle-free highways for long-range material transportations, allow swarming bacteria to perform population-level communications, and imply the essential role of the fluid phase on the emergence of large-scale synergy.

Single Nanoparticle Tracking Reveals Efficient Long-Distance Undercurrent Transport in Upper Fluid of Bacterial Swarms.

Liquid-liquid phase separation (LLPS) underlies the formation mechanism of membraneless biomolecular condensates locally to perform important physiological functions such as selective autophagy, but little is known about the relationship between their dynamic structural organization and biophysical properties. Here, a dark-field microscopy based single plasmonic nanoparticle tracking (DFSPT) technique was introduced to simultaneously monitor the diffusion dynamics of multiple gold nanorod (AuNR) probes in a protein LLPS system and to quantitatively characterize the spatiotemporal heterogeneity of the LLPS condensates during their phase transformation. Based on spatially and temporally resolved analysis of the diffusional behavior of the AuNRs, structure and material properties of p62 condensates, such as the viscoelasticity, the compartmentalization, and the recruitment of protein-covered nanoparticles into the large droplet, have been observed. Moreover, the nonsmooth droplet interface, its solidification after further phase transition or maturation, and the size effect of the inner vacuoles have also been revealed. Our method can be potentially applied to  investigation of different reconstituted membrane-free biomolecular condensates and  study of their dynamic evolution.

Real-Time Study of Protein Phase Separation with Spatiotemporal Analysis of Single-Nanoparticle Trajectories.

The formation of biomolecular condensates is driven by liquid-liquid phase separation, which is prevalent in cells to govern crucial cellular functions. However, understanding the properties of phase-separated condensates remains very challenging for the lack of suitable techniques. Here, we report a photoluminescence lifetime imaging method for real-time monitoring of phase-separated condensates, both in vitro and in living cells, using a microsecond-scale photoluminescence lifetime probe based on iridium complex. The probe has a large Stokes shift, excellent cell permeability, and minimal cell autofluorescence interference. With this method, the dynamic process of phase separation of fused in sarcoma protein has been well explored, showing high spatiotemporal resolution and high throughput. Beginning with initial formation, the protein droplets get bigger and more viscous, and then a final maturation to solidified aggregates has been characterized. This study paves the path for a deeper understanding of the properties of phase-separated biomolecular condensates.

Article	Year
Single Nanoparticle Tracking Reveals Efficient Long-Distance Undercurrent Transport in Upper Fluid of Bacterial Swarms. iScience\| PubMed ID: 31765993	2019
Real-Time Study of Protein Phase Separation with Spatiotemporal Analysis of Single-Nanoparticle Trajectories. ACS nano\| PubMed ID: 33348982	2021
Dynamic Monitoring of Phase-Separated Biomolecular Condensates by Photoluminescence Lifetime Imaging. Analytical chemistry\| PubMed ID: 33512148	2021

Jianfeng Xue

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