op-ivm-combining-vitro-maturation-after-oocyte-retrieval-with

OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery

The use of in vitro maturation (IVM) before gynecological operation (OP-IVM) is an extension of conventional IVM that combines IVM following oocyte ...

Peking University Third Hospital

National Clinical Research Center for Obstetrics and Gynecology

Key Laboratory of Assisted Reproduction, Ministry of Education

Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction

Chinese Academy of Medical Sciences

To evaluate the feasibility and value of abdominal ultrasound guided access for transvaginal hydrolaparoscopy (THL).

Trans-abdominal ultrasound guided transvaginal hydrolaparoscopy is associated with reduced complication rate.

To study the consistency of hysteroscopy findings and histological chronic endometritis (CE) in recurrent implantation failure (RIF) cases, and to compare their values in indicating antibiotic treatment.

The hysteroscopy and histological diagnosis and treatment value of chronic endometritis in recurrent implantation failure patients.

Preoperative incisional local anaesthesia with ropivacaine is a common method of providing post-laparoscopy pain relief. The pulmonary recruitment manoeuvre also provides pain relief, but the combined effect of these two methods on pain following laparoscopic procedures has not been reported. We investigated the efficacy of combining local anaesthetic infiltration of ropivacaine with pulmonary recruitment manoeuvre on postoperative pain following diagnostic hysteroscopy and laparoscopy.

Combined incisional ropivacaine infiltration and pulmonary recruitment manoeuvre for postoperative pain relief after diagnostic hysteroscopy and laparoscopy.

To evaluate the effects of laparoscopic surgery under total intravenous anesthesia (TIVA) on outcomes of heterotopic pregnancies which need to receive laparoscopic procedures.

[Effect of laparoscopic surgery under total intravenous anesthesia on postoperative pregnancy outcomes of heterotopic pregnancies].

Few studies have evaluated Hedgehog (Hh) signaling pathway activation in different types of ovarian tumors including benign, borderline and malignant ovarian tumors. The present study investigated the expression of Hh signaling pathway components (SHH, SMO, PTCH, and GLI1) in 193 ovarian epithelial tumor specimens (including 147 malignant epithelial ovarian cancers, 30 borderline ovarian tumors, 16 benign ovarian epithelial tumors) and 11 normal ovarian epithelial tissues by immunohistochemistry. The results demonstrated widespread expression of Hh pathway molecules in ovarian tumors. However, there was no significant difference in the expression intensity of SHH among the four groups (P>0.05). Statistically significant differences were identified in the expression intensity of the SMO, PICH and GLI1 among groups (P<0.001). In addition, significant differences were also revealed in the expression levels of SMO (P=0.013) and GLI1 (P=0.0005) between the platinum drug-sensitive and drug-resistant groups. The overexpression of SMO and GLI1 was further confirmed in the cisplatin-resistant ovarian cancer cell line A2780/DDP by immunofluorescence, flow cytometry and western blotting. The results revealed that the Hh pathway components SMO, PICH and GLI1 are activated in ovarian epithelial tumors. Novel potential associations between cisplatin resistance and the overexpression of SMO and Gli1 in malignant epithelial ovarian cancer were also observed, which may provide an innovative approach to the treatment of drug resistant ovarian epithelial cancer.

Activation of hedgehog signaling and its association with cisplatin resistance in ovarian epithelial tumors.

In vitro maturation (IVM), the maturation in culture of immature oocytes, has been used in clinic for more than 20 years. Although IVM has the specific advantages of low cost and minor side effects over controlled ovarian stimulation, the prevalence of IVM is less than 1% of routine in vitro fertilization and embryo transfer techniques in many reproductive centers. In this review, we searched the MEDLINE database for all full texts and/or abstract articles published in English with content related to oocyte IVM mainly between 2000 and 2016. Many different aspects of the IVM method may influence oocyte potential, including priming, gonadotrophin, growth factors, and culture times. The culture conditions of IVM result in alterations in the oocyte or cumulus cell transcriptome that are not observed under in vivo culture conditions. Additionally, epigenetic modifications, such as DNA methylation or acetylation, are also different between in vitro and in vivo cultured oocytes. In sum, current IVM technique is still not popular and requires more systematic and intensive research to improve its effects and applications. This review will help point our problems, supply evidence or clues for future improving IVM technique, thus assist patients for fertility treatment or preservation as an additional option.

Current perspectives on in vitro maturation and its effects on oocyte genetic and epigenetic profiles.

There is limited literature investigating the effects of body mass index (BMI) and androgen level on in vitro fertilization (IVF) outcomes with a gonadotropin-releasing hormone (GnRH)-antagonist protocol in polycystic ovary syndrome (PCOS). Androgen-related variation in the effect of body mass index (BMI) on IVF outcomes remains unknown.

Article	Year
Trans-abdominal ultrasound guided transvaginal hydrolaparoscopy is associated with reduced complication rate. European journal of obstetrics, gynecology, and reproductive biology\| PubMed ID: 22289262	2012
The hysteroscopy and histological diagnosis and treatment value of chronic endometritis in recurrent implantation failure patients. Archives of gynecology and obstetrics\| PubMed ID: 24395012	2014
Combined incisional ropivacaine infiltration and pulmonary recruitment manoeuvre for postoperative pain relief after diagnostic hysteroscopy and laparoscopy. Chinese medical journal\| PubMed ID: 24571870	2014
[Effect of laparoscopic surgery under total intravenous anesthesia on postoperative pregnancy outcomes of heterotopic pregnancies]. Zhonghua yi xue za zhi\| PubMed ID: 26879792	2016
Activation of hedgehog signaling and its association with cisplatin resistance in ovarian epithelial tumors. Oncology letters\| PubMed ID: 29552194	2018
Current perspectives on in vitro maturation and its effects on oocyte genetic and epigenetic profiles. Science China. Life sciences\| PubMed ID: 29569023	2018
Body mass index and basal androstenedione are independent risk factors for miscarriage in polycystic ovary syndrome. Reproductive biology and endocrinology : RB&E\| PubMed ID: 30454060	2018

Xueling Song

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