chemical-conjugation-purified-dec-205-directed-antibody-with-full

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells <em>In Vitro</em> and <em>In Vivo</em>

Targeted antigen delivery to cross-presenting dendritic cells (DC) in vivo efficiently induces T effector cell responses and displays a valuable approach ...

Helmholtz Centre for Infection Research

Synthetic mimetics of the CD4-binding site of HIV-1 gp120 are promising candidates for HIV-1 entry inhibition, as well as immunogen candidates for the elicitation of virus-neutralizing antibodies. On the basis of the crystal structure of gp120 in complex with CD4, we have used a recently introduced strategy for the generation of structurally diverse scaffolds to design and synthesize a scaffolded peptide, in which three fragments, making up the sequentially discontinuous binding site of gp120 for CD4, are presented in a nonlinear and discontinuous fashion through a molecular scoffold, which restrains conformational flexibility. The affinities of this molecule to CD4, as well as to the broadly neutralizing antibody mAb b12, whose epitope overlaps the CD4-binding site of gp120, were determined in competitive binding assays.

Article	Year
A rationally designed synthetic mimic of the discontinuous CD4-binding site of HIV-1 gp120. Journal of receptor and signal transduction research\| PubMed ID: 17118792	2006
Synthetic mimetics of the CD4 binding site of HIV-1 gp120 for the design of immunogens. Angewandte Chemie (International ed. in English)\| PubMed ID: 17211914	2007

Tatjana Hirsch

Biography

JoVE Journal Publications

Other Publications