methane-hydrate-crystallization-on-sessile-water-droplets

Methane Hydrate Crystallization on Sessile Water Droplets

This paper describes a method to form methane hydrate shells on water droplets. In addition, it provides blueprints for a pressure cell rated to 10 MPa ...

Georgia Institute of Technology

Gas clathrates are both a resource and a hindrance. They store massive quantities of natural gas but also can clog natural gas pipelines, with disastrous consequences. Eco-friendly technologies for controlling and modulating gas clathrate growth are needed. Type I Antifreeze Proteins (AFPs) from cold-water fish have been shown to bind to gas clathrates via repeating motifs of threonine and alanine. We tested whether proteins encoded in the genomes of bacteria native to natural gas clathrates bind to and alter clathrate morphology. We identified putative clathrate-binding proteins (CBPs) with multiple threonine/alanine motifs in a putative operon () in metagenomes from natural clathrate deposits. We recombinantly expressed and purified five CbpA proteins, four of which were stable, and experimentally confirmed that CbpAs bound to tetrahydrofuran (THF) clathrate, a low-pressure analogue for structure II gas clathrate. When grown in the presence of CbpAs, the THF clathrate was polycrystalline and platelike instead of forming single, octahedral crystals. Two CbpAs yielded branching clathrate crystals, similar to the effect of Type I AFP, while the other two produced hexagonal crystals parallel to the [1 1 1] plane, suggesting two distinct binding modes. Bacterial CBPs may find future utility in industry, such as maintaining a platelike structure during gas clathrate transportation.

Mainly on the Plane: Deep Subsurface Bacterial Proteins Bind and Alter Clathrate Structure.

Undisturbed frozen samples can be efficiently obtained using the artificial ground freezing method. Thereafter, the restoration of in situ conditions, such as stress and density after thawing, is critical for laboratory testing. This study aims to experimentally explore the effects of thawing and the in situ stress restoration process on the geomechanical properties of sandy soils. Specimens were prepared at a relative density of 60% and frozen at -20 °C under the vertical stress of 100 kPa. After freezing, the specimens placed in the triaxial cell underwent thawing and consolidation phases with various drainage and confining stress conditions, followed by the shear phase. The elastic wave signals and axial deformation were measured during the entire protocol; the shear strength was evaluated from the triaxial compression test. Monotonic and cyclic simple shear tests were conducted to determine the packing density effect on liquefaction resistance. The results show that axial deformation, stiffness, and strength are minimized for a specimen undergoing drained thawing, restoring the initial stress during the consolidation phase, and that denser specimens are less susceptible to liquefaction. Results highlight that the thawing and stress restoration process should be considered to prevent the overestimation of stiffness, strength, and liquefaction resistance of sandy soils.

Article	Year
Mainly on the Plane: Deep Subsurface Bacterial Proteins Bind and Alter Clathrate Structure. Crystal growth & design\| PubMed ID: 33414686	2020
Evaluation of Thawing and Stress Restoration Method for Artificial Frozen Sandy Soils Using Sensors. Sensors (Basel, Switzerland)\| PubMed ID: 33803436	2021

Jongchan Kim

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