whole-ovary-immunofluorescence-clearing-multiphoton-microscopy-for

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Female fertility and reproductive lifespan depend on the quality and quantity of the ovarian oocyte reserve. An estimated 80% of female germ cells ...

The Jackson Laboratory

We have generated a WEE-1.3 strain in  wherewe have endogenously tagged the C-terminus with GFP In this publication we demonstrate that this new strain exhibits the same expression localization pattern as the WEE-1.3 antibody and N-terminally endogenously GFP-tagged WEE-1.3 strain that have been previously published. We also show for the first time that endogenously tagging WEE-1.3 at either termini does not affect the reproductive function of the worms.

Ruby Boateng

Biography

JoVE Journal Publications

Other Publications