open-source-toolkit-benchtop-carbon-fiber-microelectrode-array-for

Open-source Toolkit: Benchtop Carbon Fiber Microelectrode Array for Nerve Recording

Conventional peripheral nerve probes are primarily fabricated in a cleanroom, requiring the use of multiple expensive and highly specialized tools. This ...

University of Michigan, Ann Arbor

Bioimpedance spectroscopy (BIS) has shown significant potential in many areas of medicine to provide new physiologic markers. Several acute and chronic diseases are accompanied by changes in intra- and extracellular fluid within various areas of the human body. The estimation of fluid in various body compartments is therefore a simple and convenient method to monitor certain disease states. In this work, the design and evaluation of a BIS instrument are presented and three key areas of the development process investigated facilitating the BIS measurement of tissue hydration state. First, the benefit of incorporating DC-stabilizing circuitry to the standard modified Howland current pump (MHCP) is investigated to minimize the effect of DC offsets limiting the dynamic range of the system. Second, the influence of the distance between the bioimpedance probe and a high impedance material is investigated using finite element analysis (FEA). Third, an analytic compensation technique is presented to minimize the influence of parasitic capacitance. Finally, the overall experimental setup is evaluated through ex vivo BIS measurements of porcine spleen tissue and compared to published results. The DC-stabilizing circuit demonstrated its ability to maintain DC offsets at less than 650 μV through 100 kHz while maintaining an output impedance of 1 MΩ from 100 Hz to 100 kHz. The proximity of a bioimpedance probe to a high impedance material such as acrylic was shown to increase measured impedance readings by a factor of 4x as the ratio of the distance between the sensing electrodes to the distance between the bioimpedance probe and acrylic reached 1:3. The average parasitic capacitance for the circuit presented was found to be 712 ± 128 pF, and the analytic compensation method was shown to be able to minimize this effect on the BIS measurements. Measurements of porcine spleen tissue showed close correlation with experimental results reported in published articles. This research presents the successful design and evaluation of a BIS instrument. Specifically, robust measurements were obtained by implementing a DC-stabilized current source, investigating probe-material proximity issues and compensating for parasitic capacitance. These strategies were shown to provide tissue measurements comparable with published literature.

Design of Bioimpedance Spectroscopy Instrument With Compensation Techniques for Soft Tissue Characterization.

Orbital atherectomy is a catheter-based minimally invasive procedure to modify the plaque within atherosclerotic arteries using a diamond abrasive crown. This study was designed to investigate the crown motion and its corresponding contact force with the vessel. To this end, a transparent arterial tissue-mimicking phantom made of polyvinyl chloride was developed, a high-speed camera and image processing technique were utilized to visualize and quantitatively analyze the crown motion in the vessel phantom, and a piezoelectric dynamometer measured the forces on the phantom during the procedure. Observed under typical orbital atherectomy rotational speeds of 60,000, 90,000, and 120,000rpm in a 4.8mm caliber vessel phantom, the crown motion was a combination of high-frequency rotation at 1000, 1500, and 1660.4-1866.1Hz and low-frequency orbiting at 18, 38, and 40Hz, respectively. The measured forces were also composed of these high and low frequencies, matching well with the rotation of the eccentric crown and the associated orbital motion. The average peak force ranged from 0.1 to 0.4N at different rotational speeds.

Experimental investigation of the abrasive crown dynamics in orbital atherectomy.

The Kirschner wire (K-wire) is a common bone drilling tool in orthopedic surgery to affix fractured bone. Significant heat is produced due to both the cutting and the friction between the K-wire and the bone debris during drilling. Such heat can result in high temperatures, leading to osteonecrosis and other secondary injuries. To reduce thermal injury and other high-temperature associated complications, a new K-wire design with three notches along the three-plane trocar tip fabricated using a thin micro-saw tool is studied. These notches evacuate bone debris and reduce the clogging and heat generation during bone drilling. A set of four K-wires, one without notches and three notched, with depths of 0.5, 0.75, and 1mm, are evaluated. Bone drilling experiments conducted on bovine cortical bone show that notched K-wires could effectively decrease the temperature, thrust force, and torque during bone drilling. K-wires with notches 1mm deep reduced the thrust force and torque by approximately 30%, reduced peak temperatures by 43%, and eliminated blackened burn marks in bone. This study demonstrates that a simple modification of the tip of K-wires can effectively reduce bone temperatures during drilling.

Notched K-wire for low thermal damage bone drilling.

As access to patient emboli is limited, embolus analogs (EAs) have become critical to the research of large vessel occlusion (LVO) stroke and the development of thrombectomy technology. To date, techniques for fabricating standardized human blood-derived EAs are limited in the variety of compositions, and the mechanical properties relevant to thrombectomy are not quantified.

Article	Year
Design of Bioimpedance Spectroscopy Instrument With Compensation Techniques for Soft Tissue Characterization. Journal of medical devices\| PubMed ID: 26029317	2015
Experimental investigation of the abrasive crown dynamics in orbital atherectomy. Medical engineering & physics\| PubMed ID: 27160429	2016
Notched K-wire for low thermal damage bone drilling. Medical engineering & physics\| PubMed ID: 28462826	2017
Standardized Fabrication Method of Human-Derived Emboli with Histologic and Mechanical Quantification for Stroke Research. Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association\| PubMed ID: 33066901	2020

Albert J. Shih

Biography

JoVE Journal Publications

Other Publications