Moxibustion Strategies for Mice

The field of moxibustion research is expanding, with a rapid increase in publications in recent years. Moxibustion is a therapy that ignites moxa on the ...

Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China

Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital

Manganese chloride (MnCl) has been shown to inhibit the Yes-associated protein (YAP) in high-fat diet-fed ApoE mice. Although YAP has been implicated in atherogenesis, there are limited data on the effects of MnCl on cardiac remodeling. In this study, we discovered, by electrocardiography, that hyperlipidemia led to spontaneous supraventricular arrhythmia (SVA) in ApoE (KO) mice, with 3 of 9 KO + MnCl mice (33%) exhibiting lower incidence of spontaneous SVA than KO mice (6 of 10 mice, 60%). Echocardiography revealed that reduced systolic function in KO mice was reversed by MnCl treatment. Oil Red O staining of the aortas and biochemical analysis of lipid levels showed that MnCl inhibited plaque formation in a lipid metabolism-independent manner. MnCl inhibited inflammatory cell infiltration and reduced fibrosis, as evidenced by hematoxylin and eosin, immunohistochemical and Masson's trichrome staining, respectively. Our findings demonstrate that spontaneous SVA and reduced systolic function were blocked by MnCl. Our findings show that MnCl was useful in delaying cardiac remodeling and reducing susceptibility to spontaneous SVA in a mouse model of hyperlipidemia.

Oral administration of MnCl attenuated hyperlipidemia-related cardiac remodeling in ApoE mice.

The therapeutic effect of grain-sized moxibustion (GS-Moxi) on inflammatory pain has been well recognized clinically, but the mechanism remains unclear. STIM1/ORAI1 is a sensible temperature channel, therefore; this study aimed to investigate the analgesic effect of GS-Moxi and the association with STIM1/ORAI1 expression. CFA-induced inflammatory pain model was established and was treated with GS-Moxi after 3 days of CFA injection. The behavioral test was measured after the GS-Moxi; then, serum was prepared for IL-1β, IL-6, and TNF-α, and the stimulated skin was used for measuring STIM1 and ORAI1 expression. The results indicated GS-Moxi had an analgesic effect on inflammatory pain and the heat variation was significant for the analgesia. GS-Moxi decreased the expression of IL-1β, IL-6, and TNF-α. Immunofluorescence and western blot analysis illustrated that heat change was associated with the stimulation of STIM1 and ORAI1. Suggesting that heat variation created by GS-Moxi could be crucial in this therapy and STIM1 and ORAI1 were potential enhancers in regulating analgesia of GS-Moxi.

Article	Year
Oral administration of MnCl attenuated hyperlipidemia-related cardiac remodeling in ApoE mice. Journal of pharmacological sciences\| PubMed ID: 33451751	2021
The role of STIM1/ORAI1 channel in the analgesic effect of grain-sized moxibustion on inflammatory pain mice model. Life sciences\| PubMed ID: 34102196	2021

Xiao-qin Dai

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